We narrowed to 310 results for: construct
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TypeCollection...neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab. A useful “starter kit”...
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Viral Production
TypeCollection...determine recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector ... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...comments on your manuscript is a form of (hopefully constructive) conflict. As you perform the experiments to... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...Valentovich LN. "DNA tuner: a computer program for the construction of polylinker sequences of molecular vectors... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post... design, it is advisable to generate multiple constructs with different insertions, since it is difficult... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read about Tags for Protein Purification... -
Evolution of Lab Techniques
TypeBlog Post.... Kulkarni. "Overview: engineering transgenic constructs and mice." Current Protocols in Cell Biology ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well... -
Adenovirus Guide
TypeGuide...linearized with the restriction enzyme PmeI . This construct is then transformed into AdEasier-1 cells (Addgene...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293...expressing E1 are transfected with the linearized construct, and rAdV vectors can be harvested about 7–10 ...purposes. The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene...adding 2.7 kb of packaging space. However, these constructs must be transfected into HER911 E4+ cells for...Ad2 are the most commonly used for rAdV vector construction, due to their high gene transfer efficiency.... -
Ras Pathway
TypeCollection...Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene expression vectors for in... -
Control AAV Preps
TypeCollection...None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...Schmitz, Jonathan E., et al. "Rapid DNA library construction for functional genomic and metagenomic screening... -
Plasmids 101: Repressible Promoters
TypeBlog Post...stage-specific promoter and design a UAS reporter construct, the reporter will only be expressed where and... -
Neurodegeneration Research Collection
TypeCollection...Synuclein, and more. New and Noteworthy: Use tau constructs to study aggregation. Saha et al. Nat Commun.... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...to know (and understand!) the hardware types, construction, and testing used for delivering your light ... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...expression in your experiment. Some inducible constructs can exhibit leaky expression in the absence of... -
Quick Guide to All Things Lentivirus
TypeBlog Post...the different vectors used, lentiviral vector constructs have proven very useful due to their ability ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post... the recombination of the double-floxed opsin construct, thereby enabling its expression under a strong... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...Pre-constructed pairs of TALEN plasmids targeting zebrafish genes....