...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency (x-axis) which SNPs ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...

...are five more model organisms that have left a historical impact on the scientific community. (And, check...  The E. coli genome has an extensive research history and characterization. Many proteins and processes...organisms in the world. With its extensive scientific history, highly characterized genome, and widespread utilization...organisms is its thoroughly developed and defined history in research (Krämer, 2015). As a result, a vast...

...that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing...technological innovations. Let’s take a look at three historical techniques: southern blotting, restriction mapping...throughout the world. Let us know your favorite "Historical Lab Technique" in the comments section below!...Benjamin Chain. "The sequence of sequencers: The history of sequencing DNA." Genomics (2015). PubMed PMID...

...Stephen M., et al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. ...Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications...and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission to the British Journal...

... tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  Each...described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee et al.(1) assessed...

...selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine...promote replication.  Selection markers for yeast Historically, scientists have utilized auxotrophic selection...

...a nucleus -> DNA compaction around histones -> Examples of histone-modifying enzymes and reader-effector... specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these modules...

...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

....8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579 (red) 464 (... 3.9 h (red) pMedium-FT-N1 pTRE-Medium-FT pBAD/HisB-Medium-FT Slow-FT 402 (blue), 583 (red) 465 (...2.6/4.6 28 h (red) pSlow-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein...

...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

...long, whistling while we work. This is certainly not the truth (partially because I can’t whistle). No,...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...

... Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency of events... “true” signal.  Figure 8: A series of histograms from Alyamani et al., 2018, showing number of...

...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ...

...BFP mutant (BFPH66) contains a histidine at amino acid 66. This histidine is encoded by a ‘CAC’ codon that...C-to-T base editing event. This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66...

... your plasmid or carrying out your experiment. History of E. coli strains E. coli are gram-negative, rod... DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers...researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred...

...ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds...and using an epitope-based strategy such as Anti-6xHis. You could also consider strategies using other ....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...

...also take a look back on some fascinating Addgene history that you might not have known about. Here's the...Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid...

...expression via epigenetics with the addition of histone modifiers. “We want to allow people to prove the...476 (22 August 2013).   , with the addition of histone modifiers, ...

...We do this by promoting citation, supporting sophisticated search, and curating plasmid collections. Addgene's...whether the plasmid has been used in your system. Sophisticated search You can search for specific plasmids ...

...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution A Powerful...