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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 412 results for: his

Showing: 261 - 280 of 412 results

  1. A Control for All Seasons

    Type
    Blog Post
    ...Stephen M., et al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. ...Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications...and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission to the British Journal...

  2. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  Each...described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee et al.(1) assessed...

  3. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine...promote replication.  Selection markers for yeast Historically, scientists have utilized auxotrophic selection...

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...a nucleus -> DNA compaction around histones -> Examples of histone-modifying enzymes and reader-effector... specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these modules...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ....8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579 (red) 464 (... 3.9 h (red) pMedium-FT-N1 pTRE-Medium-FT pBAD/HisB-Medium-FT Slow-FT 402 (blue), 583 (red) 465 (...2.6/4.6 28 h (red) pSlow-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ...

  11. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency of events... “true” signal.  Figure 8: A series of histograms from Alyamani et al., 2018, showing number of...

  12. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... your plasmid or carrying out your experiment. History of E. coli strains E. coli are gram-negative, rod... DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers...researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred...

  13. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds...and using an epitope-based strategy such as Anti-6xHis. You could also consider strategies using other ....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...

  14. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...also take a look back on some fascinating Addgene history that you might not have known about. Here's the...Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid...

  15. Let There Be LITE Plasmids

    Type
    Blog Post
    ...expression via epigenetics with the addition of histone modifiers. “We want to allow people to prove the...476 (22 August 2013).   , with the addition of histone modifiers, ...

  19. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ... polymers that can work with all the bells and whistles. The fibers are made from a process called thermal...additional components are added - the ‘bells and whistles’ – including microscale light emitting diodes,...
Showing: 261 - 280 of 412 results