... the Maxiprep protocol, scale the reagent volume and column number as needed. The use of Mega or Gigapreps... plateau and sometimes fall off entirely if the column or reagents are significantly overloaded. If you...Consult the manufacturers handbooks for appropriate volumes and numbers of tandem purifications. Visit our ...

...many kits cannot handle more than a certain total volume of gel per reaction. Place the gel in a labeled... the gel is directly proportional to its liquid volume and this is used to determine how much of each ...sample you want to load on a gel, make 10% more volume than needed because several microliters can be ...

...1 1 μL AgeI add ddH 2 O to bring to 50 μL final volume Incubate at 37°C for 2 hours. Purify with Qiaquick... DNA ligase add ddH 2 O to bring to 20 μL final volume Incubate at 16°C for 4-20 hours. Transform 2 μL...0.8 μL NcoI add ddH 2 O to bring to 20 μL final volume Incubate at 37°C for 1-2 hours. 4. Run the digestion...master mix to each tube from step c for a total volume of 100 μL. Pipette master mix directly into the...

...200 mL of agar in 500 mL bottles. The extra empty volume is necessary to prevent your molten agar from boiling...Cover the opening of the bottle with its cap or aluminum foil (but do not make an air-tight seal!) and ...the first plate to get a good idea of what that volume looks like in your particular plate. For the remainder...

...loosely cover the entire top of the bottle with aluminum foil. Autoclave and allow to cool to room temperature... swirl. Loosely cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate ...

...Inactivate T4 Pol by heating to 75° for 20 minutes. Volume (μl) Reagent Final Conc. 4 10X Buffer 1X 20-30 ...annealing reaction. This should be done in a small volume with no additional water (<5 μl). It is advisable...

...often an extra tube filled with water to the same volume as your samples. If your samples are significantly...need to fill the blank based on weight instead of volume. Pro-Tip For microfuge tubes, place them with the...

...with videos for select protocols in the right-hand column. You can find even more video content on the Addgene...recombinant antibodies using Protein A or Protein G columns Watch the Video! Coomassie Purity Stain Determine...

...you to recover all transformants. If the culture volume is too big, gently collect the cells by centrifugation...centrifugation and resuspend in a smaller volume of LB so that there isn't too much liquid media on the agar...

...of HCl at a time. Add deionized water to a final volume of 1 L and recheck pH to ensure that it has not...up to 4 times for a total of 16% of the culture volume. At 72-96 h post-transfection, supplement the flask...

... . Simply adjust the mass of agarose in a given volume to make gels of other agarose concentrations (e.g...sample you want to load on a gel, make 10% more volume than needed because several microliters can be ...

...to a microfuge tube. Add 8 µL of PBS to a total volume of 13 µL . Prepare the recombinant antibody Dilute... issues. Plot the mg/mL versus the HC + LC Area column for the IgG standards in Google Sheets. Open exported...

...purity. Note: Purity is measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for...

... mostly absorbed (there is a very small visible volume of pooled liquid remaining on the surface). We ...

...concentrations. We recommend mixing 2μg each in a total volume of 50μL - add additional annealing buffer if necessary...

... of reagent that will be resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place...

...and vector to use for the ligation reaction. The volume of vector DNA and insert DNA used in the ligation...

...Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume 500 μL *Plasmid concentrations and ratios should...

...sample. To ensure that your sample is adequately illuminated, look through the eyepiece. Adjust the power ...