We narrowed to 541 results for: SHI
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TypeBlog Post...focus of our first decade. We did this the old-fashioned way: through physical outreach, visiting organizations... our repository grew to over 150,000 plasmids, shipped out our two millionth item, and had a cumulative...
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Developing Lab Management Software for Biology
TypeBlog Post...the appropriate stab culture Grow the stab culture Ship the stab culture to the proper location To keep...technicians to retrieve the sample and get it ready for shipping. In the first case, we naively assume that everything... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...plasmids from this publication at Addgene. 2. Nishimasu, Hiroshi, et al. "Crystal structure of Cas9 in complex... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post... research environment” with a strong focus on publishing, but still made it so she could “get exposure...work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...Undergraduate Biology Education: A Call to Action, Washington, DC. 2. President's Council of Advisors on Science...Science, Technology, Engineering and Mathematics, Washington, DC: US Government Office of Science and Technology... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...Poster layout While it’s good to let your ingenuity shine, and you generally have free reign over how you ...catchy, but beware. A distracting background or clashing colors can take away from what should be the focus... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post... editing while still allowing on-target editing (Shin et al., 2017). Acrs could also be used to reduce....e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu J-J, Bray NL, Rauch BJ, Baik SH,... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...strong signal. However, exposure time and its relationship to signal depend on what you’re comparing your...working with Dr. Wu please contact us here! Ava Shipman is a research assistant in lab of Zhuhao Wu at ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...categorization is complex Plasmid classification has shifted over the years, and new classifications are still...organization doesn’t necessarily reflect genetic relationships or plasmid incompatibility. Plasmids may also... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...over 100 scientific institutions have proposed a shift to recombinant DNA-based antibody technologies (...practical choice. Hybridomas are much tougher to ship, often lack sequence data, and may genetically drift... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...upstream of the PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases...publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou ... -
Plasmids 101: FLEx Vectors
TypeBlog Post...cell populations. Acknowledgement:Through a partnership with genOway, we are able to distribute materials...Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui Zeng Additional... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...fruit flies before, get yourself a copy of Fly Pushing by Ralph J. Greenspan. It’s a fantastic book for...thanks to our guest blogger, Jon Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post... science. Prepare the night before: To avoid rushing out the door without your poster, place it by your... you may receive. People often ask about the relationship of your work to recently published papers or... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post... as a result of an insertion, a deletion or a frameshift (Ding et al., 2014, Wang et al., 2015). In these...available at Addgene and could be useful to labs wishing to create this kind of animal models. Start using... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...MagLOV. Andrew was thrilled to see that MagLOV’s “astonishing” magnetoresponse was clearly visible whether ...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness of fluorescence all ... significantly brighter and available with red-shifted fluorophores (find FRET capable GEVIs here!). The... -
PhD Applications After COVID
TypeBlog Post...the question “So, what comes next?” For those finishing up an undergraduate education or ending a Master... Bastin is a first year PhD student at George Washington University whose research focuses on Drosophila-parasite... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...1999. 17(10): p. 969-73. PubMed PMID: 10504696. 4. Ohashi, T., et al., An experimental study of GFP-based... -
Experimenting with New Careers while in Grad School
TypeBlog Post... Brain Scoop YouTube channel @Ehmee, and David Shiffman @WhySharksMatter and see what kind of content ... been the best supporter and most productive partnership CINSO has had. Volunteer! The turning point in...