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  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources at Addgene...them attention they righlty deserve, but, in this post, we'll provide a small sampling of the many amazing...at [email protected] and maybe we can write a blog post about it! Split Cas9 system The Cas9 protein is...sub-domains. It has also been used to observe intra- and post-Golgi segregation of cargo during their transport...about these light-switchable TCSs, read our blog post: Synthetic Photobiology: Optogenetics for E. coli...more information on inntags, read our recent blog post. Georgieva, et al. Nature Methods. 2015 Aug 31...using traditional cloning strategies may seem preposterous. Luckily, the Pierre Neveu laboratory designed...

  2. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...experiment are known as your "panel". In this blog post, we'll discuss the principles and process of designing...resolve this issue. Make sure to check out our blog post on color compensation to learn more about them. ...bd-spectrum-viewer.   Conclusion You made it! In this post, you learned about the principles behind fluorophores...

  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan Ward who is an assistant...the majority of knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more..., some of the “elegans” methods outlined in this post – particularly the use of co-selection methods –...

  4. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...This post was contributed by guest blogger Stephanie Hays, a researcher at the University of California...expected. Jennifer Stancill, Ph.D., is currently a post-doctoral fellow at Vanderbilt University. She studies... Resources on the Addgene Blog Read Our Previow Post on the March for Science Learn How Future of Research...chair of the postdoctoral engagement committee and active member of the university's Postdoctoral Association...in the veracity of its work. Alina Chan is a postdoctoral researcher at Harvard Medical School working...runs hand in hand with policy. Alexandra is a postdoctoral researcher in the University of Michigan Department...continue the dialogue. Erin Golden is currently a postdoctoral fellow at the University of Colorado Anschutz...

  6. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...This guest post was contributed by Sana Khan Khilji, a PhD student at the Max Planck Institute of Colloids...progress.Learn more about cell culture contamination in this post from the ATCC Declutter! Keep the hood clean Hoods...

  8. Of Myc and Men

    Type
    Blog Post
    ...into play? We’ll dive into those questions in this post as we explore… the life and times of the myc tag...substance P has an amide group added to its C-terminus post-translationally, which turned out to be critical...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...second chance” mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence...1053 aa/3.2 kb) with AAV vectors for efficient postnatal editing. LbCpf1 and AsCpf1 are 3.7 kb and 3.9 ...

  10. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...make the most of these pages, we've written this post to guide you through them and make the best use ...publications which have used the plasmid (see our blog post) for more information about this feature!).  This...

  11. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other ...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, Alán, et al...Addgene blog Read all of our viral vectors blog posts Find tips for getting started with viral vectors...

  13. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...CRISPR blog posts Download our CRISPR cheat sheet Read our science communication blog posts Resources ...past 20 years has been aimed at grad students, postdocs, and other working scientists, creating a presentation...

  14. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...resources on the Addgene blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR...

  15. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...transferable skills guide Read all of our career blog posts Resources on Addgene.org Learn more about careers...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    ...please sign up here.  Here's what you'll find in this post: Yeast-based protein evolution Fluorescent tools...from Chertkova et al., 2020. In their BioRxiv post, the authors generated and described several improved...

  18. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ... including plants, mammals, and insects. In this post, we will compare two of the most widely used systems... time, or reagents and equipment. Hopefully this post has provided some insight on these differences in...

  19. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...cells, but mammalian cells can perform human-like post-translational modifications that bacterial and most...effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are not equally...

  21. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...libraries can be quite challenging to use! In this post, I’ll walk you through how to prep and transduce...perform your pooled library screen…but that’s a blog post for another day.  Using pooled libraries can be ...

  22. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...applications from across disciplines now available. In this post, we’ll review the inspiration behind ExM, how it...samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should start...

  23. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...This post was contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes ...be protected from this dangerous material. This post was contributed by the Mesothelioma Cancer Alliance...

  24. DNA Purification Without a Kit

    Type
    Blog Post
    ...acid purification methods not mentioned in today’s post? Let us know in the comments! Additional resources...protocols and tips Browse our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular...

  27. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...experiment, they definitely made my life easier!  In this post, I’ll share some AAV production, purification, and...account for batch-to-batch variability. Check out this post to learn more about different ways to titer AAV....
Showing: 421 - 450 of 783 results