...metabolites that are dynamically secreted by muscle during exercise to meet the brain’s physiological demands,” ...protects the brain from depression. Mice that are genetically modified to produce extra PGC-1a1 only in their..., the brain is protected from stress-induced biochemical changes that could otherwise lead to depression...

...Emeritus at UCSF. Dr. Bourne has some radical (and some not so radical) suggestions for defining the problem... training too many scientists and some propose radical changes to the way we view the graduate school ...University founded a successful consulting company called The Balsa Group. University of Pennsylvania has...

...paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash, Stanford University...as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron resolution...I have worked many hours in a dark room with a confocal microscope, yet I was once again fascinated by...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...

...excellent Technical knowledge and creativity Become a “thought leader,” develop a technical “niche,” develop...FierceBiotech to “learn the business” Attend events with local networking and trade organizations Another excellent... be found on the UK Career Site for Researchers called Vitae. Vitae recently published a career development...

...pride in facilitating the exchange of not just the physical samples, but also the legal documentation that... Addgene has adopted the terms of the Uniform Biological Material Transfer Agreement (UBMTA) for its material... may not use the material in human subjects or clinical trials Acknowledgement in recipient’s future...have opted into our eMTA system can even sign electronically or choose to auto-approve MTAs. Whatever your...

.... All of this requires genes to be regulated dynamically.” To understand how that works in memory formation...they could package LITEs in viral vectors and genetically target them to specific cell populations. They... the genetic and epigenetic players in normal biological processes and disease states. While LITE plasmids...with LITE? Reference: Silvana Konermann et al. "Optical control of mammalian endogenous transcription and...

...Antibody characterization is critical to enhance reproducibility in biomedical research. eLife, 13, e100211...launched AAV Packaged on Request, a service that dramatically expands and simplifies your viral vector options..., K., Alshafie, W., Baker, M. R., Ball, A. R., Callahan, D., Cooper, J. A., Crosby, K., Harvey, K. J.,...

... therapies,” said Kris Wood of Duke University Medical Center. “While we do not yet have a comprehensive...resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully, the researchers...generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and it took them... he said. “It could [be used to screen for] morphological features, migration, or survival in different...

...while - a tool to expand perception, which is ironically what science is about. For me it is essential...living organism I study, to be able to see it all scales. Most modern biologists focus on one aspect alone... time, defend the thesis, etc. Photography is critically important in many fields of biology and I believe...appreciated by a wider audience for more than just its biological importance. More of Daniel's stunning work can...

... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...the lab with parties in the lab’s common areas, local pubs, and homes. The Church lab also toasts a newly... Improving your work-life balance Balancing the Scale: NSF's Career Life-Balance Initiative Can Scientists...

...notebooks and binder on your desk. Arrange them in chronological order (or by project, if that works better for...upcoming grant deadlines, and sync everything to your calendar. Ditch the paper To Do List and move to one that...reminders. These programs can be synced to your online calendar and can be accessed from your computer or smartphone... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...

...nearly all domains of life repair genomic damage, specifically double strand breaks. Researchers have long ...by direct transformation. A given insert will typically contain both a protein modification sequence and... not only track the abundance, movement, and localization of individual proteins, but also investigate... green, and red FPs that are codon optimized specifically for expression in yeast. These tagging vectors...

...scientists are overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It...shown. Top: The follower network of life (green), physical (blue) and social (orange)  scientists largely...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist...

...imaging method that uses iterative staining and chemical bleaching to allow for high-resolution imaging...not recommended reagents) along with information critical for the performance of a reagent, e.g., antigen... (2022). IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse...Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial...

...expression of the indicators over white dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and...feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors cannot be...nLightR, both generating a measurable signal after electrical stimulation with a single pulse, while nLightR...Cui, G., Lin, D., Du, J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific...

... may not even get a solution to the problem you called about. Next, you try an email, given that this ...that can help you through any search, ordering, technical, or quality control issues. Improving customer...chance to stop what you’re doing and make a phone call or write out an email. You need help right at the...support to address this exact problem, and it’s called “self-service.” The idea here is to build features...

...successfully resolved loci with physical map distances of 75 and 2 Mbp, with the calculated fluorescent distances...makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has many applications, the...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart...

... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) who took the time...choices.” She points out that a PhD teaches analytical and critical thinking skills that are applicable to ...

... (PA) near-infrared fluorescent proteins (FPs), called PAiRFP1 and PAiRFP2, to the in vivo imaging toolbox...of specific wavelength and intensity allows for optical labeling and tracking of proteins, organelles, ...resolution images in vivo. “One of the reasons we can’t localize one cell at a depth of one centimeter with fluorescence... are always looking to improve upon existing genetically-encoded probes by making them brighter, more ...

...May 22, 2019 Smart Servier Medical Art This site has 3,000 free medical images organized into four main... over 20,000 icons is designed by professional medical illustrators with input from life scientists. It... anatomy and the human body, cellular biology, medical specialities, and general items. Individual images...

...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever been...Bioinformatics reached out to us to tell us about a resource called Cellosaurus, a growing cell line information database....  For those wanting to access Cellosaurus programatically, their API allows fine grained query and retrieval...

...techniques in the biological sciences and constitute foundational elements of biomedical research. Traditional...Digestion products are then transformed directly into chemically competent E. coli and transformants selected ...procedure to work properly. Directly transform chemically competent cells with the digested mixture. There...

...over a month ago I finished up my PhD in Biological and Biomedical Sciences at Harvard University and entered...researchers gain doing PhDs (i.e. technical writing, teaching, presenting, analytical thinking, time management...

...gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19. Immunol...DNA while eliminating some of the drawbacks. Specifically, viruses are able to infect and replicate in...Anderson Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as...

...problems, develop general technical proficiency, communicate your work, maintain ethical standards and interact...? Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... you admire her management style? Does he have ethical behavior? Does the lab encourage diversity? Knowing...

... tactics of prokaryotes to deliver the technological biological blockbuster known as the CRISPR (clustered...destroy the phage upon re-exposure (2, 3). More specifically, specialized Cas proteins snip the foreign DNA...programmable platform well-suited for various innovative biological and translative technologies. Addgene has empowered...

...makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system...from a single construct. DNA and gRNA are not to scale.   Let’s start with the simplest multiplexing...multiplexing plasmid is available from the Koffas lab. Scaling up your multiplexed gRNAs with Golden Gate and ...and Gibson assembly methods If you want to scale up the number of gRNAs in your plasmid, you’ll need to ... combinatorial mammalian genetics.” Journal of Clinical Investigation 125(4) (2015):1603-19. PubMed PMID...

...FRET reporters. mTFP1 is also insensitive to physiologically relevant changes in pH. Find mTFP1 plasmids...Fluorescent proteins that oligomerize often affect the localization and/or function of the protein that they are...fluorescent protein and found that the fusion protein localized to their expected locations. An analysis of mWasabi...characterization and applications in fluorescence imaging." Biochemical Journal 400.3 (2006): 531-540. PubMed PMID: 16859491...

... years of exclusively writing lab reports and empirical essays had also stirred some of my more creative...exhibitions, animation, graphic design, and more. I recall a particularly busy point of the first semester... notion of “heading” in soccer as a possible neurological health risk. Having played the sport my entire...among collision sport athletes, as well as the ethical and health policy implications arising from sport-related...

... defined learning program. Learners cannot (theoretically, at least) fully and freely engage or disengage...Informal education is education that is provided, typically ad hoc, for learners who can choose to engage ...would use “PCR test” to mean “a test that uses a biological sample to look for presence of a virus.” A scientist... scientist underrepresented minorities in your local high school who are also students in AP biology ...

...editing of transformants then typically occurs in T0 plants (from callus) or T1 plants (Arabidopsis floral...involves killing the untransformed seedlings or callus (undifferentiated plant cell culture), which is...cacti and beets. Betanin belongs to a different chemical family than the red/purple plant pigments found...

...bonds. This post focuses on "sticky end" TOPO (also called TOPO-TA) cloning; however, the TOPO cloning technique...to facilitate replication. The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I... has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading functionality are...are planning on transforming your reaction into chemically or electro-competent E. coli - excess salt causes...

... PA will put their award to work on a condition called xeroderma pigmentosum (XP). This recessive genetic...gene encodes a sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they...Wattanasirichaigoon carried different mutations in a gene called SOX10. To understand the functional role of those...models carrying different SOX10 mutations. More specifically, they’ve proposed to use plasmids with modified...

... The conventional approach has typically been to either systematically delete/overexpress endogenous genes... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

...Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1...fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited PSAM AAV...

...cell death" plays a pivotal role in an array of biological processes including development, the immune system...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters... reporter for imaging apoptosis in vivo." Cell chemical biology 23.7 (2016): 875-882. PubMed PMID: 27447051...apoptosis in animals." Journal of the American Chemical Society (2019). PubMed PMID: 30821975. Additional...

...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without...Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor labeling...variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific loci based on the guide RNA sequence...RFP) to the genomic loci of interest. Pairs of identical hairpins within one gRNA will result in a blue...

...from Massachusetts General Hospital and Harvard Medical School took a closer look at the AAV vectors that...Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving iodixanol gradients...exo-AAVs seem to target cell types with a similar specifically as conventionally produced AAV particles. But... Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development 9 (2018): 278-287. PubMed PMID: 29707602...

...test if a type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But...Then, one of them heard about our online platform, called Crowdfight, and filed a request for help. In just... stage of the project we are focusing on what we call “asymmetric collaborations.” These are transient...-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations...

...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene ... with biorender.com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends ...elements of AAV and rAAV vectors, modifications can drastically affect gene transfer efficiency and transgene...serotypes vary in their gene transfer efficiency. Our practical knowledge of ITRs is still limited, but this area...