We narrowed to 881 results for: NSI

1. Addgene Stands with the Scientific Community

   Type

   Blog Post

   ...archiving their biological materials during funding transitions. Enabling resource sharing: Our operating model...

2. The Addgene Antibody Data Hub

   Type

   Blog Post

   ... a report with a DOI for citation. If you’re considering requesting an Addgene antibody, the Data Hub ...

3. MassBioEd Career Exploration Day at Addgene

   Type

   Blog Post

   ...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from Our Bloggers...

4. 500,000 Plasmids Shipped and Counting!

   Type

   Blog Post

   ...plasmid shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. ...

5. What's New in CRISPR - May 2019

   Type

   Blog Post

   ...dinucleotide PAM (N4CC), lending to high target site density (average every 8 bp). Nme2Cas9 is also small enough...

6. Plasmid Preps: Different Purity, Different Quantities, Different Uses

   Type

   Blog Post

   ...plasmid from a bacterial culture, it is important to consider your experiment.  It will dictate the amount of...

7. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

   Type

   Blog Post

   ... might suspect contamination in your sample or consider asking your sequencing facility to apply a special...

8. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

   Type

   Blog Post

   ...but only in the genome of a group of Archaea. Consistent with this origin, the authors suggest that Cas14...

9. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

   Type

   Blog Post

   ...nuclear translocation rates are another variable to consider when designing KTR experiments. Third, KTRs are...

10. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type

    Blog Post

    ...Laboratory Medicine. He attends in the Medical Intensive Care Unit, the Pulmonary Consultation Service,...

11. Addgene's Top Blog Posts from 2020

    Type

    Blog Post

    ...DNA? Here are Five DNA Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate...

12. Science Communication Snapshot: DayCon 2015

    Type

    Blog Post

    ...science, but they didn’t seem to mind! Attendees consistently mentioned how pleased they were to learn about...

13. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type

    Blog Post

    ... life sciences. In this post, we'll give you an inside look at the Top 10 plasmid technologies distributed...

14. Reflections and Looking Ahead

    Type

    Blog Post

    ...and developers to unlock the potential of our extensive plasmid database — because we believe that innovation...

15. Editor's Choice, September 2016

    Type

    Blog Post

    ...reasons: 1) I think phage are super cool and have considered getting a tattoo of my favorite molecular biology...

16. Our Most Popular Blog Posts from 2019

    Type

    Blog Post

    ...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and...

17. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type

    Blog Post

    ...Medical Center. “While we do not yet have a comprehensive understanding of how cancers become resistant...

18. Management for Scientists: Seeking Feedback

    Type

    Blog Post

    ...volunteer constructive feedback and suggestions. Consider offering rewards (gift card, afternoon off, etc...

19. Six Spooky Science Stories and Halloween at Addgene

    Type

    Blog Post

    ...Spinochordodes tellinii‘s larvae makes its home inside grasshoppers and crickets. When it’s ready to transform...

20. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type

    Blog Post

    ... ask for help with various issues, the PI is responsible for determining meaningful solutions. PI-trainee...

21. Easing the Protein Purification Process with pCri

    Type

    Blog Post

    ...Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications...

22. What's New in CRISPR - September 2019

    Type

    Blog Post

    ...the mechanism behind its activity including an extensive RNA scaffold and a domain needed for DNA unwinding...

23. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type

    Blog Post

    ... Here’s what caught our eye recently (not a comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA...

24. What's New in CRISPR - March 2020

    Type

    Blog Post

    ...expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian transcriptional repressor...

25. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type

    Blog Post

    ... Blog Post on Our Citations Feature Browse More Inside Addgene Posts Additional Resources on Addgene....

26. A Better Way to Get Customer Support: The Help Center

    Type

    Blog Post

    ...first. Resources on the Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid...

27. 6 Steps to Submitting a Resume That Gets Seen

    Type

    Blog Post

    ...dramatically increase the chances of being seriously considered for an open position.  Check out Joanne's Reddit...

28. An Addgene Summer Internship

    Type

    Blog Post

    ...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...

29. Bringing Sustainable Practices to the Lab: Recycling

    Type

    Blog Post

    ... already leading the cultural change toward responsibility in our lab’s environmental impact, but we can...

30. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type

    Blog Post

    ...calculator Promega's Biomath Calculators Primer resuspension calculator (sign-in required.)  DailyCalcs Science...

31. CRISPR 101: Drug discovery and CRISPR

    Type

    Blog Post

    ...mutational basis of a disease is known – repeat expansion, gene knock out, etc. – CRISPR can be used to ...

32. Bricking Science: Portraying Scientific Reality Through LEGO

    Type

    Blog Post

    ...is the most approachable for LEGOs. Stop motion consists of taking many pictures, one for each position...

33. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    ...configuration. In addition, D10A editing efficiency is consistently higher than that of H840A, especially with smaller...

34. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type

    Blog Post

    ...sonification. Though it helps capture weaker or more transient DNA-protein interactions, the reliance on fixation...

35. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type

    Blog Post

    ...post-grad plans, but in the end, writing won out. “I considered teaching at a liberal arts school but I’ve always...

36. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type

    Blog Post

    ...product launch. “The writing group I am part of is responsible for checking the scientific accuracy of everything...

37. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type

    Blog Post

    ... cry genes from the soil bacterium Bacillus thuringiensis and analysing the insecticidal potential of ...

38. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type

    Blog Post

    ...positive selection strategy, here are a few to consider. Antibiotic selection Selection is straightforward...

39. Cellosaurus: A Cell Line Information Database

    Type

    Blog Post

    ...information in the resource.  This is a labor-intensive project; scripts can be used in some cases, but...

40. Understanding GPCR Signaling Using TRUPATH

    Type

    Blog Post

    ...(Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and...

41. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type

    Blog Post

    ...gene which differ between the AAV serotypes are responsible for serotype-specific differences in tissue and...

42. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type

    Blog Post

    ... genetic makeup, overcomes all of these labor-intensive and invasive techniques to reliably label any ...

43. Plasmids 101: What is a plasmid?

    Type

    Blog Post

    ...scientists can track their location or quantity inside a cell Plasmids 101: Green Fluorescent Protein...

44. Networking and Inspiring Young Scientists at Freshers Fairs

    Type

    Blog Post

    ... develop their career skills for positions both inside and outside academia. Inspiring future scientists...

45. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    ...social and regulatory reasons. 1.) The transposon consists of a mirrored set of inverted repeats (green) ...

46. Management for Scientists: Managing vs. Leading

    Type

    Blog Post

    ... by Peter Fuda and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals...

47. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type

    Blog Post

    ...first for our repository, and I was proud to be responsible for sharing some of our data at this meeting....

48. Choosing a Good Mentor for Scientists

    Type

    Blog Post

    ... may hedge on their answers to your questions. Consider reading Toxic Academic Mentors by @drmellivora...

49. New Viral Vectors - Winter 2025

    Type

    Blog Post

    ... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers...

50. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    ...labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6 bp fixed region...