Addgene

We narrowed to 394 results for: AGA

Showing: 281 - 300 of 394 results

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The Materials Science of Optogenetics Experiments

Type

Blog Post

...opportunity to “reset” and become receptive to light once again. Therefore, light must be delivered in pulses. An...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

...most popular with our requestors. However, we can again see quick adaptation and steady use of all types...
Viral Vectors 101: Voltage Indicators

Type

Blog Post

...action potentials which are electrical charges propagated through multiple cells to do things like translate...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Type

Blog Post

...University in Canada. He's happy to be contributing again to science. Acknowledgements Chan Zuckerberg ...
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

Type

Blog Post

...colony phenotype upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction of ...
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

Type

Blog Post

...Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional Resources on...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

... to prevent any non-recombinant vectors from propagating. The ligation-independent methods like Gibson...
10 Basic tips for mammalian cell culture

Type

Blog Post

... Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids 101: Mammalian Vectors Resources...
Plasmids 101: Protein Expression

Type

Blog Post

...transformed into a bacterial cell. Transformed cells propagate, are induced to produce your protein of interest...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

...to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

...price point, which should be carefully balanced against the limitations of the assay. Fig....
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...PCR product is often visualized on a diagnostic agarose gel to confirm product presence, size, and relative...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

... right places. Checks and balances: protecting against cancerous cells Luckily, there are several safety...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

... and development before it can truly be pitted against CRISPR head-to-head. Exciting features of NgAgo...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...other words, these parts can be used over and over again in combination with novel genes to get very precise...
Great Results Start with Great Standard Curves

Type

Blog Post

...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...
Simplify Cloning with in vivo Assembly

Type

Blog Post

...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Type

Blog Post

...with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant role in library...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...

Showing: 281 - 300 of 394 results

Results per page:

The Materials Science of Optogenetics Experiments

Twenty Years of Addgene Sharing: CRISPR

Viral Vectors 101: Voltage Indicators

CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

Plasmids 101: 5 factors to help you choose the right cloning method

10 Basic tips for mammalian cell culture

Plasmids 101: Protein Expression

AAV Vector Quality Control: Going the Extra Mile with NGS

PCR Applications for Copy Number Variation Assays

Polymerase Chain Reaction Overview and Applications

Addgene's Cancer Collection Pages Connect the Research Community

Google Forums Round Up: First Impressions of NgAgo

Tips for Using BLAST to Verify Plasmids

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Great Results Start with Great Standard Curves

Simplify Cloning with in vivo Assembly

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

A Guide to Starting Your Own Journal Club

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