We narrowed to 398 results for: AGA

1. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   ... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR in a “second chance” mechanism...

2. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...desribed how P. furiosus has systems to defend against both DNA and RNA viruses - 2 cas systems regulate...

3. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ...fluorescent units and so lowers the FRET signal back again. The Ting lab constructed 2 versions of this reporter...

4. The Materials Science of Optogenetics Experiments

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   Blog Post

   ...opportunity to “reset” and become receptive to light once again. Therefore, light must be delivered in pulses. An...

5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg ...

6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...colony phenotype upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction of ...

7. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...PCR product is often visualized on a diagnostic agarose gel to confirm product presence, size, and relative...

8. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional Resources on...

9. PCR Applications for Copy Number Variation Assays

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   Blog Post

   ...price point, which should be carefully balanced against the limitations of the assay.      Fig....

10. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ... to prevent any non-recombinant vectors from propagating. The ligation-independent methods like Gibson...

11. 10 Basic tips for mammalian cell culture

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    Blog Post

    ... Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids 101: Mammalian Vectors Resources...

12. Plasmids 101: Protein Expression

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    Blog Post

    ...transformed into a bacterial cell. Transformed cells propagate, are induced to produce your protein of interest...

13. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ...action potentials which are electrical charges propagated through multiple cells to do things like translate...

14. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ...most popular with our requestors. However, we can again see quick adaptation and steady use of all types...

15. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post

    ...to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it...

16. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ... right places. Checks and balances: protecting against cancerous cells Luckily, there are several safety...

17. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ... and development before it can truly be pitted against CRISPR head-to-head.  Exciting features of NgAgo...

18. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...

19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...other words, these parts can be used over and over again in combination with novel genes to get very precise...

20. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...