We narrowed to 768 results for: Dos
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TypeCollection...protospacer sequence) is G, do not add another G ( e.g., sgRNA-B in Table 4 ) and do not add C to the final...
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Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog Post...prepared me that those challenges would be from doing a remote internship during a pandemic. Before I ...role in the company. One of the things I wanted to do was create content that was unique but also brand... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post... job descriptions clearly describe that the roles do not involve bench research. I am always surprised... 5) Apply for the job as requested in the posting Do this online and/or via email exactly as the job posting... -
Science as Art
TypeBlog Post...received over 2,000 entries. Do you mix your passion for art and science? Do you look for the beauty in ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...blue to ultraviolet light, whereas chromoproteins do not fluoresce at all or fluoresce at very low levels...identified in sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may... mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression ... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...it. Biotech companies are known to have tools that do this internally, but they are not available publicly...advised, there is little detail published on how to do it best. Scientists are typically advised to mutate...boosted our productivity in the Biohub. We hope it will do the same for you. Send us your questions or comments... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...and tell your audience just that. Explain what you do know about a related topic, and let them know while...overwhelming than stepping into a sea of posters – where do you begin? Design a game plan Prior to attending ...poster you want to know more about? Interested in doing a postdoc in a presenter’s lab? Want to collaborate... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post... on the inside of the cell. However, assuming you do need to permeabilize, you have a few options for ...organellar membranes. Created with BioRender. How do I choose?! Ok, so now you know some of the possible...possible types of fixatives or permeabilizers, but how do you decide which ones to use? Here are some tips ... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...Accuracy needed Do you need to call exact copy numbers or just general trends? How confident do you need to...researchers can use to quantify said copies. They do so using copy number variation (CNV) assays. There... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...which are doing important work in our microbiomes. Given that viruses are everywhere and doing everything...we try to use them in an experiment and they don't do anything. Viruses are complicated and they perform... -
Plasmids 101: Colony PCR
TypeBlog Post...before using them to screen colonies. The best way to do this is by using your vector with and without an ...clones for later use. There are a few ways you can do this. If you are going to complete your colony PCR...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about ... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...times). I guess it's cool to be popular, but how do I know if a plasmid is actually useful to me? Plasmid... I want to use this plasmid in a publication, how do I know who to acknowledge? Depositing lab: The name...Our Walkthrough of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...deposit had already moved on to their next position. We do routinely review deposits that are left in the hold...materials the way we will with journal articles. But we do encourage you to consider sharing them openly as ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...disease. Figure 1: Scientists are continuing to do essential research into COVID-19 (like here at Addgene...boundaries. To be able to beat this virus, we will need to do the same with science,” said Guillaume Bourque, a... -
Working 9-5: an Addgene Co-op
TypeBlog Post...shadowing members of my team to learn what I was to do for my time at Addgene. This training prepared me...where each team gives a presentation on what they do and how it relates to other teams at the company.... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog Post...questions about scientists on Twitter: who are they? What do they share? And how they are connected? Here are ... (left), life (center), and physical (right). How do you use Twitter as a scientist? If you don’t use ... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...at Addgene. You are the reason we’re able to do what we do! Special Thanks Trophies and engraving... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...publications relevant to your research interests. We do this by promoting citation, supporting sophisticated...can put straight into your reference section. Why do this? Not only does citing plasmids from Addgene ... -
A Better Way to Get Customer Support: The Help Center
TypeBlog Post...you may not have the chance to stop what you’re doing and make a phone call or write out an email. You...which you can describe your issue and press send. Doing so will bring your concern directly to our support... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog Post...education? Let us know in the comments section below! Do you know of a society or event which would benefit...While we are not able to help with every request, we do aim to support events and scientific community activities...