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We narrowed to 772 results for: Dos

Showing: 281 - 300 of 772 results

  1. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...and tell your audience just that. Explain what you do know about a related topic, and let them know while...overwhelming than stepping into a sea of posters – where do you begin? Design a game plan Prior to attending ...poster you want to know more about? Interested in doing a postdoc in a presenter’s lab? Want to collaborate...

  2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ... on the inside of the cell. However, assuming you do need to permeabilize, you have a few options for ...organellar membranes. Created with BioRender.   How do I choose?! Ok, so now you know some of the possible...possible types of fixatives or permeabilizers, but how do you decide which ones to use? Here are some tips ...

  3. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Accuracy needed Do you need to call exact copy numbers or just general trends? How confident do you need to...researchers can use to quantify said copies. They do so using copy number variation (CNV) assays. There...

  4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...blue to ultraviolet light, whereas chromoproteins do not fluoresce at all or fluoresce at very low levels...identified in sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may... mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression ...

  5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...it. Biotech companies are known to have tools that do this internally, but they are not available publicly...advised, there is little detail published on how to do it best. Scientists are typically advised to mutate...boosted our productivity in the Biohub. We hope it will do the same for you. Send us your questions or comments...

  6. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...which are doing important work in our microbiomes. Given that viruses are everywhere and doing everything...we try to use them in an experiment and they don't do anything. Viruses are complicated and they perform...

  7. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...before using them to screen colonies. The best way to do this is by using your vector with and without an ...clones for later use. There are a few ways you can do this. If you are going to complete your colony PCR...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about ...

  8. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...times).     I guess it's cool to be popular, but how do I know if a plasmid is actually useful to me? Plasmid... I want to use this plasmid in a publication, how do I know who to acknowledge? Depositing lab: The name...Our Walkthrough of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene....

  9. Guide to Using Pooled Libraries

    Type
    Guide
    ...: Apply library Apply selection Most cells die or do not pass selection (in the case of a reporter) Sort... screen, the goal is to identify those cells that do not survive the selection mechanism. You’ll infect...

  10. Antibody Guide

    Type
    Guide
    ...comes from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will...direct or indirect detection method? If indirect, do you already have secondary antibodies available in...have identical features and epitopes, to ensure you do not validate the same antibody multiple times. You...antibodies will also have a source species. What conjugate do you want to use? Most secondary antibodies will be...buy small aliquots of many different antibodies and do batch validation up front rather than risk delaying...

  11. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...questions about scientists on Twitter: who are they? What do they share? And how they are connected? Here are ... (left), life (center), and physical (right). How do you use Twitter as a scientist? If you don’t use ...

  12. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...shadowing members of my team to learn what I was to do for my time at Addgene. This training prepared me...where each team gives a presentation on what they do and how it relates to other teams at the company....

  13. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...deposit had already moved on to their next position. We do routinely review deposits that are left in the hold...materials the way we will with journal articles. But we do encourage you to consider sharing them openly as ...

  17. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...you may not have the chance to stop what you’re doing and make a phone call or write out an email. You...which you can describe your issue and press send. Doing so will bring your concern directly to our support...

  18. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...education? Let us know in the comments section below! Do you know of a society or event which would benefit...While we are not able to help with every request, we do aim to support events and scientific community activities...

  20. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...and institutions are not aware of what Addgene can do for them and how the repository can help them share...other side of the force and as an Addgenie I will do all my best to help them in their tasks. But I won...
Showing: 281 - 300 of 772 results