We narrowed to 444 results for: ENA

1. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   ...research. Homology directed repair (HDR), which enables insertion of custom genomic modifications, is very...

2. Hot Plasmids: FRET-Based Biosensors

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   ...acceptor domains. He says, these new colors might now enable observation of the interaction between different...

3. Learning Technical Skills at a Lab Central Workshop

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   ...accounts. When I heard about how LabCentral aims to enable emerging biotech companies, I was excited to go...

4. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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   ...create an array of multicolor reporters that will enable imaging of multiple proteases at once (Zhang et...

5. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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   ... Listen to Our Interview with Previous Awardee Kwabena Duedu Resources at Addgene.org Check out our ...

6. Visualizing Translation at the Single Molecule Level

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   ...appear yellow, and translated RNAs appear red. To enable this color switching, TRICK requires the following...

7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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   ...iGluSnFR3 in your lab The new iGluSnFR3 variants should enable scientists to robustly detect signaling events ...

8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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   ...together Combining in silico and in vitro approaches enables researchers to develop safer and more effective...

9. Hot Plasmids - January 2023

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   ...only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and other chaotropic...

10. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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    ...linked to these metadata and publications help to enable reproducibility of experiments. Additionally, proper...

11. Plasmids 101: NGS Quality Control for Pooled Libraries

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    ...proper use requires careful amplification and maintenance of good representation. Addgene wishes you luck...

12. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    ...Image from Richardson et al., 2016.   dCas9 enables sequence replacement without cutting Our in vitro...

13. Components of CRISPR/Cas9

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    ... which will be described in more detail below, enables Cas9 to cut a specific genomic locus of many possible...

14. Synthetic Photobiology: Optogenetics for E. coli

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    ...as with other plasmid-based systems, which will enable the assembly of larger systems. The work of Schmidl...

15. Plasmids 101: Mammalian Vectors

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    ...is typically more (double) than that used for maintenance of a transfected cell line.  Keep these tips ...

16. A Novice's Perspective on Neuroscience - SfN2017

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    ...biological fields the number potential of experiments enabled by physical, software, and biology-based tools ...

17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    ...unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity and sgRNA...

18. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

19. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    ...light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back...

20. Targeted Mutagenesis with EvolvR

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    ...Shakked O., et al. "CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable...