We narrowed to 671 results for: FIE

... Center patient databases, this research team identified 1,049 perturbations in several genes spanning... 33 cancer types. The types of mutations they identified included gene amplifications and fusions, missense...

... are then lysed and the protein of interest is purified using an affinity tag. Additional tags may be ...is present, TEV can remove these tags from the purified protein. For more detailed information on these...

... COVID-19 strives to match up scientists across fields with different technical knowledge that could help...sharing resources for COVID-19 research in their field including datasets and platforms for discussing ...

...-effective screening methods, David Liu’s lab identified and validated CBEs with reduced Cas9-independent...Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive Cas enzymes can alter...

...system for gene transfer, studying the stability of modified Ti plasmids during plant infection, as well as...the constitutively active M24 promoter has been modified with duplicated enhancer domains and characterized...

...expression system pDawn to clone and express newly identified proteins and enzymes. These compounds will later... scientists working in a wide range of research fields and geographical locations. We are excited to see...

...CAST, the nuclease-deficient CRISPR-Cas system identifies and binds a genomic target using a gRNA, and ...sequences into genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly...

...could be beneficial for other scientist’s in your field, while providing you with visibility. For instance...ReFigure also makes it easier for experts in a given field to share their specialized knowledge of which findings...

...linked to the original publication via its PubMed identifier, which can also be used to search the entire ...ORCS has a dedicated summary page with PubMed identifiers and links provided, in addition to a list of ...

...assignments. The instructors are leaders in the field of microbiology with multiple high-profile publications...should be able to be enjoyed and appreciated by all fields not solely the specific area the writer works.  ...

...community. For our first concrete steps into this field, we’ve curated microbiology plasmids from the repository...network with scientists in this diverse and exciting field and to find out how we can serve them better. Here...

...content coming also keeps me on top of a variety of fields thereby broadening my knowledge. In addition, this...distillation, etc) are transferable to many different fields. If you start thinking about preparing yourself...

...look as you expected. Once you have cut out and purified your insert and recipient plasmid backbone bands...diagnostic restriction digest of 100-300ng of your purified DNA with the enzymes you used for the cloning....

... and downstream (3’) of the DNA segment to be amplified. When these reagents are combined in an appropriate... After the PCR is complete, the product can be verified based on size by gel electrophoresis . Protocol...

...dissociate under blue light. This tool has now been modified by Torsten Wittmann’s lab at UCSF to modulate ...of the LOVTRAP domains. When both halves of the modified EB1 protein are overexpressed in cells, microtubule...

...and PfAgo, another thermophilic Argonaute. They identified NgAgo and showed that it can cleave plasmid and...removing 1-20 nucleotides from the cleavage site specified by the gDNA. Depending on your application, this...

...of the primer that binds to the sequence to be amplified (usually 18-21bp) When selecting restriction... primer that hybridizes to the sequence to be amplified (the ORF in this case), not the Tm of the entire...

... as a seizure disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3...specifically, they’ve proposed to use plasmids with modified nickase Cas9 protein in order to target the specific...

...et al. made the surprising discovery that both purified CPP-tagged protein and an untagged control could...subsequently enter the nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. ...

...inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is necessary for removal of GFP...the mCherry labeled mRNA and keeps it in a single field of view for constant tracking. You can then directly...