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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 671 results for: FIE

Showing: 281 - 300 of 671 results

  1. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...references. Collaborate with another lab, in another field maybe, and publish Join a lab that has multiple ...committee) Follow industry publications like FierceBiotech to “learn the business” Attend events with local...

  2. Let There Be LITE Plasmids

    Type
    Blog Post
    ... via epigenetics with the addition of histone modifiers. “We want to allow people to prove the causal ...August 2013).   , with the addition of histone modifiers, ...

  4. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ... are then lysed and the protein of interest is purified using an affinity tag. Additional tags may be ...is present, TEV can remove these tags from the purified protein. For more detailed information on these...

  6. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...-effective screening methods, David Liu’s lab identified and validated CBEs with reduced Cas9-independent...Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive Cas enzymes can alter...

  7. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...system for gene transfer, studying the stability of modified Ti plasmids during plant infection, as well as...the constitutively active M24 promoter has been modified with duplicated enhancer domains and characterized...

  10. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...assignments. The instructors are leaders in the field of microbiology with multiple high-profile publications...should be able to be enjoyed and appreciated by all fields not solely the specific area the writer works.  ...

  11. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...CAST, the nuclease-deficient CRISPR-Cas system identifies and binds a genomic target using a gRNA, and ...sequences into genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly...

  13. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...community. For our first concrete steps into this field, we’ve curated microbiology plasmids from the repository...network with scientists in this diverse and exciting field and to find out how we can serve them better. Here...

  15. Lentivirus Production

    Type
    Protocol
    ...) transfection protocol. This procedure can be modified for alternative packaging cell lines or transfection...without limiting cell growth. Left panels: bright field images; right panels: GFP channel images. Last reviewed...

  17. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...and PfAgo, another thermophilic Argonaute. They identified NgAgo and showed that it can cleave plasmid and...removing 1-20 nucleotides from the cleavage site specified by the gDNA. Depending on your application, this...

  18. Plasmid Cloning by PCR

    Type
    Blog Post
    ...of the primer that binds to the sequence to be amplified (usually 18-21bp) When selecting restriction... primer that hybridizes to the sequence to be amplified (the ORF in this case), not the Tm of the entire...

  20. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...et al. made the surprising discovery that both purified CPP-tagged protein and an untagged control could...subsequently enter the nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. ...
Showing: 281 - 300 of 671 results