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  1. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified version of CAG promoter that the Zhang lab has...transfect a variety of mammalian cells and can be modified for viral trasduction (see the Zhang Lab's Nature...

  2. Science Communication for Everyone

    Type
    Blog Post
    ...mathematicians. We hope to highlight every science field as we expand and encourage more direct conversations... work with you? We welcome researchers from all fields of science to share their experiences and explain...

  3. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that...a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these...

  4. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...references. Collaborate with another lab, in another field maybe, and publish Join a lab that has multiple ...committee) Follow industry publications like FierceBiotech to “learn the business” Attend events with local...

  5. Let There Be LITE Plasmids

    Type
    Blog Post
    ... via epigenetics with the addition of histone modifiers. “We want to allow people to prove the causal ...August 2013).   , with the addition of histone modifiers, ...

  7. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ... are then lysed and the protein of interest is purified using an affinity tag. Additional tags may be ...is present, TEV can remove these tags from the purified protein. For more detailed information on these...

  9. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...-effective screening methods, David Liu’s lab identified and validated CBEs with reduced Cas9-independent...Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive Cas enzymes can alter...

  10. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...system for gene transfer, studying the stability of modified Ti plasmids during plant infection, as well as...the constitutively active M24 promoter has been modified with duplicated enhancer domains and characterized...

  14. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...assignments. The instructors are leaders in the field of microbiology with multiple high-profile publications...should be able to be enjoyed and appreciated by all fields not solely the specific area the writer works.  ...

  15. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...community. For our first concrete steps into this field, we’ve curated microbiology plasmids from the repository...network with scientists in this diverse and exciting field and to find out how we can serve them better. Here...

  18. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...and PfAgo, another thermophilic Argonaute. They identified NgAgo and showed that it can cleave plasmid and...removing 1-20 nucleotides from the cleavage site specified by the gDNA. Depending on your application, this...

  19. Plasmid Cloning by PCR

    Type
    Blog Post
    ...of the primer that binds to the sequence to be amplified (usually 18-21bp) When selecting restriction... primer that hybridizes to the sequence to be amplified (the ORF in this case), not the Tm of the entire...
Showing: 281 - 300 of 665 results