We narrowed to 464 results for: LARGE
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TypeBlog Post...and actual molecular size – it is possible that a large or charged FP fusion can change the subcellular ...
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Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...Permeabilization What is the point? Antibodies are large proteins that need a little help crossing cell membranes... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...amplicons. Sybr has high background noise, due to the large amount of dsDNA in most starting materials, and ... -
Plasmids 101: Colony PCR
TypeBlog Post...Tips and tricks from the bench Don't pick too large of a colony. Too many bacteria can inhibit your ...the insert site. A positive clone will produce a larger size product then a negative clone without the ...-) will not. Backbone-specific primers (2) give larger sized products for positive clones (+) compared... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post... parts. That was the motivation for releasing a large parts kit. Once you have so many parts available... -
Which Fluorescent Protein Should I Use?
TypeBlog Post... called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George...discovery, the family of FPs just keeps getting larger with hundreds of variants available. Read on to... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...occurring bacterial endonucleases that recognize a large range of DNA sequences. Given the variety of these... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol...immediately, and decontaminate as necessary. For large spills that cannot be easily cleaned, evacuate and... -
Design Tips for Prime Editing
TypeBlog Post... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker... -
Great Results Start with Great Standard Curves
TypeBlog Post...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...editor experimentalist, and especially shines once large-scale prime editing experiments come into play. ... -
Tips for Writing a Good Cover Letter
TypeBlog Post... did, and state that it would help you manage a large number of files as part of the job. Structure of... -
Hot Plasmids - November 2023
TypeBlog Post...variants use different RT enzymes and performed large edits with similar or better efficiency than PEmax... -
Viral Production at Addgene
TypeBlog Post...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...outlined below, allow for researchers to select large sets (with overhangs that violate some of the traditional... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...Interestingly, he also adds to the disproportionately large number of honorees to have trained under a previous...fortunate enough to apprentice with top scientists to larger groups of trainees at an earlier point in their... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post... Design and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis...metal ion, or fold to incorporate themselves into a larger target, such as a protein. Such flexibility makes... -
How to Write a Scientific Review Article
TypeBlog Post..., to search the literature, and to synthesize a large volume of information: basically, a scientist’s ... -
MXS Chaining
TypeBlog Post...Gibson assembly method Single reaction, can assemble large DNA sequences, no restriction enzymes needed Not...