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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 469 results for: LARGE

Showing: 281 - 300 of 469 results

  2. Pooled Library Amplifications

    Type
    Blog Post
    ... prone to recombination, while a library with a large backbone might be more difficult to transform.  ...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in...

  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave off of the protein...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ...

  8. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...Tips and tricks from the bench Don't pick too large of a colony. Too many bacteria can inhibit your ...the insert site. A positive clone will produce a larger size product then a negative clone without the ...-) will not. Backbone-specific primers (2) give larger sized products for positive clones (+) compared...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George...discovery, the family of FPs just keeps getting larger with hundreds of variants available. Read on to...

  13. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... purification procedures. Even if you produce a large amount of protein in your expression host of choice...

  15. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... are just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more... Career Expo and the ACS career fair, which are larger meetings offering resources for students seeking...

  16. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...experience. With this valuable training behind you (and largely only that is necessary), there are opportunities...to manipulate cells (manufactured by one of the larger biotech companies), and as far as I remember, it...

  20. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... Design and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis...metal ion, or fold to incorporate themselves into a larger target, such as a protein. Such flexibility makes...
Showing: 281 - 300 of 469 results