We narrowed to 630 results for: PROC

...you with some advice on the antibody selection process. Antibodies are not one size fits all When choosing...experiments are the same. Differences in sample processing, fixation, antigen-retrieval, and permeabilization...

...your ideas, invite others into decision making processes, and even interview better! As a scientist turned..., re-oriented, continued presenting. The whole process took just under a minute. I can't say the journal...

...vital for researchers to better understand the processes that happen inside the cell,” says Pedre Pérez...from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory neurons...

...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...recombinant plasmid and therefore makes the cloning process much more efficient, as one does not have to thoroughly...

... massively parallel combinatorial genetics." Proceedings of the National Academy of Sciences 111.34 (2014... CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy of Sciences113.9 (2016...

... she could “get exposure to the drug discovery process.” The ClonTracer library originally came about ...that these are selected for during the treatment process. The authors went on to show that combination treatment...

...that could influence the results? The peer-review process is not perfect, so even though it is science’s ...There are a few different ways to automate the process as well. Sign up for eTOC (electronic table of ...

...self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory actions...goals is to promote an understanding of how these processes are regulated at the cellular, tissue and organ...

... controlled by the GAL1 promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini...successfully analyze Met protein complementation. In the procedure diagrammed in Figure 3, students first transform...

...interference (DIC) microscopy and computer imaging processing. The combination of these methods provides unique...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

... the nucleotide sequence during the DNA repair process. The addition of small molecules that promote homologous...Also, because non-homologous repair is a random process, zygotes edited using the same nuclease may not...

...important roles in many cellular and regulatory processes, but much of their functionality is yet to be ... of microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. ...

.... Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can be returned...remains, you may want to repeat your stripping procedure, perhaps using longer incubation times, or try...

...2019). Finally, scientists can perform the entire process of antibody selection and maturation in vitro by...libraries of recombinant antibodies. The selection process is often performed via phage display, yeast display...

...feedback. They’ve most likely been through the process themselves many times and will have useful advice...agency’s investment. 6. Failure is part of the process, keep your head up! It’s very likely that at first...

...road ahead for students interested in leading the process despite the recent advance. Similarly, grad students...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014...

...Protein expression refers to the second step of this process: the synthesis of proteins from mRNA and the addition...Plant systems share many of the same features and processing requirements as mammalian cell expression systems...

...scientists have been able to study many cellular processes, including mitosis, mitochondrial fission/fusion...insertions can only be detected by labor-intensive processes like PCR or by evaluating visual phenotypes. Improving...

... Protocol Pages Agarose cloning method procedure The procedure (illustrated in the YouTube video below...

...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade ...needs to remain anchored to Cascade throughout the process. The reeling of ssDNA and unwinding of dsDNA occur...