We narrowed to 631 results for: PROC
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TypeBlog Post...you with some advice on the antibody selection process. Antibodies are not one size fits all When choosing...experiments are the same. Differences in sample processing, fixation, antigen-retrieval, and permeabilization...
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Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...vital for researchers to better understand the processes that happen inside the cell,” says Pedre Pérez...from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory neurons... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...your ideas, invite others into decision making processes, and even interview better! As a scientist turned..., re-oriented, continued presenting. The whole process took just under a minute. I can't say the journal... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...recombinant plasmid and therefore makes the cloning process much more efficient, as one does not have to thoroughly... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post... massively parallel combinatorial genetics." Proceedings of the National Academy of Sciences 111.34 (2014... CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy of Sciences113.9 (2016... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post... she could “get exposure to the drug discovery process.” The ClonTracer library originally came about ...that these are selected for during the treatment process. The authors went on to show that combination treatment... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...that could influence the results? The peer-review process is not perfect, so even though it is science’s ...There are a few different ways to automate the process as well. Sign up for eTOC (electronic table of ... -
Working with Nuclear Receptors
TypeBlog Post...self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory actions...goals is to promote an understanding of how these processes are regulated at the cellular, tissue and organ... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post... controlled by the GAL1 promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini...successfully analyze Met protein complementation. In the procedure diagrammed in Figure 3, students first transform... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...important roles in many cellular and regulatory processes, but much of their functionality is yet to be ... of microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. ... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post.... Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can be returned...remains, you may want to repeat your stripping procedure, perhaps using longer incubation times, or try... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...2019). Finally, scientists can perform the entire process of antibody selection and maturation in vitro by...libraries of recombinant antibodies. The selection process is often performed via phage display, yeast display... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...interference (DIC) microscopy and computer imaging processing. The combination of these methods provides unique...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post... the nucleotide sequence during the DNA repair process. The addition of small molecules that promote homologous...Also, because non-homologous repair is a random process, zygotes edited using the same nuclease may not... -
6 Tips for Grant Writing
TypeBlog Post...feedback. They’ve most likely been through the process themselves many times and will have useful advice...agency’s investment. 6. Failure is part of the process, keep your head up! It’s very likely that at first... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...road ahead for students interested in leading the process despite the recent advance. Similarly, grad students...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014... -
Plasmids 101: Protein Expression
TypeBlog Post...Protein expression refers to the second step of this process: the synthesis of proteins from mRNA and the addition...Plant systems share many of the same features and processing requirements as mammalian cell expression systems... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade ...needs to remain anchored to Cascade throughout the process. The reeling of ssDNA and unwinding of dsDNA occur... -
Common Injection Routes in Mice
TypeBlog Post...delivery is also common. Due to the intubation, this procedure is considered quite invasive. Restraint: anesthesia...similar to its intratracheal counterpart, as both procedures target the lungs. The main differences are that... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...sybody candidates. Figure 2: Outline of the process for selection of sybodies against membrane proteins...sybody), ribosome, and mRNA. During the screening process, displayed sybodies that bind to the biotinylated...