...current identification methods missing something? Here we will review some time-tested gene identification systems...reporter construct can be something phenotypic (such as a fluorescent protein or something species-specific) ...gene product. These are effective tools but neglect some biologically relevant situations. Tissue-specific...

...about anything can disrupt your bench work and sometimes no culprit can be found…. In our lab we call this...the lab gnome. See this video where he/she/it/something is captured on camera: Unfortunately, unless...them that they are not alone; a researcher doing some experiments in Brazil can have the same issues as...the potential contained within each. There must be some way to use LEGOs, which are practically universally...optimistically grasp at limitless potential, and who sometimes wish they could live differently than we do day...

...effectively with other teams. While they sometimes dig in and do some of the work, they must be more focused...current task. Most of us start as doers, some become managers and some take on leadership roles. You may be...promotion. Yet little explanation is given for how someone can or should demonstrate this quality.   I am ...

... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...viruses and focused only on those found in bacteria. Some common ones you might see include ColE1, pMB1 (which...Not all origins of replication are created equal. Some will produce many plasmid copies and others produce...antibiotic, and medium can all affect copy number. Some ori's are temperature sensitive; others ori's can...started to die off. Have any questions? Need something clarified? If so please, post in the comments....

...everyone to attend all the meetings or that there’s some correct level of intimacy that creates the magic...live audiences in your future.     3. Start something In almost every career talk I present, I’m asked...before others do”. Candidates who have “started something” which they can share on their resumes have a ...Informational interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation...and do a character count in your head.  Here are some sites that have compiled lists of grants: Science...

...As you browse the collection, you'll likely find some proteins of interest for which you have several ...the pocket of a folded protein, or blocked by something bound to the protein, the antibody can't bind ...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'...This involves using heat and/or chemicals to break some of the bonds keeping the protein folded, changing...protein and its configuration in your assay. For some assays, you may only need to validate for recognizing...

...Before we design your first panel, we will introduce some background on the color spectrum and the principles...This step is called emission. During excitation, some of the photon's energy is lost, so the energy of...commonly used fluorophores and their emission spectra. Some fluorophores carry their emission peaks within their...However, when you need to label multiple markers, some bleeding is often unavoidable. But don't worry, ...exclude APC-Cy7 from the rest of our panel. Besides some background cell death constantly happening in cell...culture, the transduction process itself also leads to some cell death. As a result, there is a high abundance...

...2013), and visualizing structural changes in the ribosome during protein translation (Ermolenko et al., ...the same exact location as the donor. As long as some of the acceptor molecules are targeted nearby (such...synthetase/tRNA pair, a researcher can lure the ribosome into inserting a fluorophore-tagged amino acid...unnatural amino acid finds the UAG codon in the ribosome, resulting in site-specific incorporation of the...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors.... "Observation of intersubunit movement of the ribosome in solution using FRET." Journal of molecular ...

...cabinets, a rickety old incubator, a few pipettes, and some home-made equipment. It was humble in comparison...members pursue a diverse range of projects at BosLab. Some are entrepreneurs using our space to perform early-stage...probiotics to filming educational lab videos, while some are looking to develop new skill sets. Kellen Andrilenas...different samples. The goal is to not only learn something about the microbial diversity in different soil... at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement   ...

...works in a lab struggles with reproducibility at some point. Usually it comes up when a researcher decides... new project and begins by trying to reproduce someone else’s result. Then, they hit trouble. The experiment...was not, and it was just that they were doing something differently. Communication comes in many forms—from...protocols are available and detailed enough for someone else to follow them, to being open and willing ...approach to doing that will be, and it will need some effort and experimentation. But it ought to be possible...

...exported as a picture. I believe that showing something different from time to time breaks up the monologue...monologue a little and gives participants something else to focus on. Use collaborative cloud apps It is... Take brain breaks Meetings can get very long sometimes, and interaction fades away the longer we are ... hour (or another designated time) of meeting, someone could raise their hands or signal the approach ...conversations. This can help team building and bring back some degrees of belonging that was probably lost during...

...other slides in your deck.  If someone asks a question about something they did not understand about your...milestone in a scientist's career is mediated by some kind of communication to another person.  In an ...piece of feedback you receive generalizes.  If someone suggests a change to a slide, think about whether...scientists with opportunities to practice communicating.  Some fantastic K12 education initiatives like BiteScis...

...cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid...which is achieved by expression of the vesicular somatitis virus G glycoprotein (VSVG). VSVG is a broad tropism...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...different tissue or cell types. AAV also requires some back up from adenovirus genes – E4, E2a, and VA ...virus (AAV). We also offer viral prep services for some of the plasmids in our collection. Want to make ...

...location and they either trace downstream from the soma (neuron cell body) toward the axon (anterograde ...tracer) or upstream from the axon terminals to the soma (retrograde tracer) (Fig. 2). Viruses as neuronal...retrograde transport efficiency in most serotypes. Some viruses (e.g., rabies) naturally exhibit retrograde...Skorput, 2022, Albaugh, 2020, Tervo, 2016).  In fact, some neuronal pathways may not be transduced at all by...you understand this new tool, go forth and trace some neurons!  This post was originally written by Leila...

...also like to put some social pressure on many other labs that may be doing something similar. There may... hundreds of transcription factors. We wanted something that would allow automation. This design cuts ... when plasmids become available.  We’ve also had some requests for variations on the plasmids we’ve developed...may be other variants of this that are better for some experiments than those we’ve put up. We would encourage...

...we’ll use mammalian plasmids to introduce you to some of your potential options and cloning methods, but...happen.  There are many reasons to multiplex gRNAs, some being: Using dual nickases to generate a knockout...number of gRNAs in your plasmid, you’ll need to use some assembly methods such as Golden Gate or Gibson assembly...constructed using oligonucleotide assembly.  Unlike some of the plasmids described above, this vector does...Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” ...Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.” Proceedings ...

...actually don’t have to worry about and that is something to recognize! Distributing plasmids, viral service...Diversification is happening. The Addgene team will have some fun challenges this year and I’m excited about this...community. In a recent law review article we received some entirely unsolicited praise: “Addgene, for one, ...to our legal team, and great customer service, somehow we have scientists actually loving the MTA?! ...program, and with other intern positions. We have an awesome talent pipeline. Turnover at Addgene is about...

...a kit that outlined several reasons why doing something without a kit has advantages: less plastic waste...and on everything, including your skin. Here are some general tips for working with RNA, even if you are...RNases are commonly found in cells, and without something to block RNase activity upon cell lysis, RNA is... strong protein denaturant would be required- something that broke down RNases before RNases had a chance...thiocyanate–phenol–chloroform extraction: twenty-something years on. Nature Protocols 1:581–585. https://...

...a journal for formal peer review. Some organizations, including some journals, also support open peer ...committees, as opposed to the opaque (and, admittedly, somewhat meaningless) practice of listing “Submitted” articles...scientific communication needs. Going to present some unpublished results at a conference? Post your working... lack of awareness of the benefits of preprints. Some worry about faulty work being posted as preprints...

...and pumps in developmental biology.  Examples of some ion-channel and ion-pump encoding genes HCN1 KCNJ2...KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction proteins cx43 GJB1 GJB2... of two cells) can also affect patterning and in some cases even override genetically encoded patterning...tools for studying bioelectricity in vivo. Here are some optogenetic and GEVI tools (as discussed in Mathews...signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity is...