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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 302 results for: SPI

Showing: 281 - 300 of 302 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...each T-175 flask: Aspirate culture media and rinse once with 10 mL of PBS. Aspirate PBS and add 2 mL of...at ~80% confluence. Aspirate culture media and rinse once with 60 mL PBS. Aspirate PBS and add 35 mL of...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ...Untransduced 0 1498.5 1.5 Mix the dilutions well Gently aspirate media from the cells. Add 1.5 mL of a viral dilution...calculating the titer. Incubate for 48–72 h. Gently aspirate media and replace with 1 mL of PBS. Calculate ...

  4. General Transfection

    Type
    Protocol
    ... the cells at 37 °C, 5% CO 2 for ~20 h. Gently aspirate media, add 10 mL fresh DMEM complete containing...following morning. The following morning, carefully aspirate the media. Replace the media with 15 mL of DMEM...

  5. Lentivirus Production

    Type
    Protocol
    ...the media and chloroquine and mix well. Gently aspirate the media out of the previously seeded 10 cm plate...following morning. The following morning, carefully aspirate the media. Replace the media with 10 mL of DMEM...

  6. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...sample. Add 2 µL 10X reducing agent to each sample. Spin the sample briefly in the microcentrifuge. Heat ...the samples for 10 min at 100 °C in a heat block. Spin the sample briefly in the microcentrifuge. Remove...

  7. Kit Free RNA Extraction

    Type
    Protocol
    ... tissue/cell lysate. Pro-Tip Having your samples spin at 4°C helps reduce RNA degradation. If you don’...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down the cultures and use a miniprep kit to obtain...media from cells and pool with media from Day 4. Spin media at 1,250 rpm for 5 minutes to pellet any HEK...

  9. Chemogenetics Guide

    Type
    Guide
    ...activated by the synthetic ligand spiradoline, however, spiradoline exhibited off-target effects in vivo...

  10. Optogenetics Guide

    Type
    Guide
    ...Examples: iChloC, SwiChRca, Phobos, Aurora Browse Channelrhodospin plasmids . Halorhodopsins Halorhodopsins are...desynchronization of neural activity, with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...tethering gene that helps facilitate integration. Despite knowing the general preference for integration,...envelope genes, and/or with site-specific injections. Despite the possibility of nonspecific transduction, lentiviral...

  12. DNA Quantification

    Type
    Protocol
    ...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

  13. Weighing Reagents Protocol

    Type
    Protocol
    ...transfer it into your container. Make sure that any spills produced during the protocol are cleaned up. Discard...

  14. Immunocytochemistry

    Type
    Protocol
    ...Section 2: Fixing and permeabilizing cells Gently aspirate the media from the 24-well plate. Wash each well...

  17. Pouring LB Agar Plates

    Type
    Protocol
    ... bit more than 200 mL (~220 mL) just in case we spill anything or have small errors in measurement. You...

  18. Plan Your Experiment

    Type
    Guide
    ...transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host ...

  19. Adenovirus Guide

    Type
    Guide
    ...diseases in humans and other animals, most commonly respiratory infections, such as the common cold. The immune...

  20. Molecular Biology Reference

    Type
    Guide
    ... selective pressure to retain the plasmid DNA, despite the added replication burden, as bacteria without...
Showing: 281 - 300 of 302 results