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We narrowed to 451 results for: abo.5

Showing: 281 - 300 of 451 results

  1. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...This is the fourth in a 5 part series on Management for scientists. Once you are responsible for managing...Medical School researcher Connie Cepko to learn about her management style. Recipes for delegation success...accountability? What types of issues do you want to know about right away? Provide adequate support. The manager...

  2. An Addgene Summer Internship

    Type
    Blog Post
    ...Syntaxin3 gene was the winner. We used the next 4-5 weeks to work on cloning the Syntaxin3 gene in a pLenti...intern coordinator shortly after hearing her talk about Addgene’s internship opportunity taking place next...

  3. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833-5 Resources on Addgene.org Optogenetics guide Chemogenetics...I often wonder about the gut-brain axis (admittedly for self-serving reasons – I want to understand my...

  4. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22 nuclear homing an artifact? Lundberg... a protocol has been established) may be less laborious than viral purification, and it does not raise...

  6. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...form it’s chromophore at a more comfortable 37°C (5). Dendra2 photoconverts from green to red fluorescence...15910. PubMed PMID:15505211. PubMed PMCID:PMC528746. 5.Gurskaya, Nadya G., et al. "Engineering of a monomeric...emitted, making it easier to capture a clear image above background. The majority of PA-FPs come in 2 flavours...Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent...

  7. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ... entering the specifics of your plasmids. Figure 5: Click the "Enter Data" button to add your plasmid...Enter the growth and distribution information. Step 5: Verification On this page you will see a preview ...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

  8. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...who knows about you. I found some other great tips for finding mentors in this recent blog 5 Strategies...work if both parties are diligent about replying and thinking about good questions and answers. Professional...Medical School researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We...Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. If your organization...much less threatening! If they say no, don’t worry about it. It is a compliment to ask someone to share their...

  9. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Labs plasmid grant we are excited to introduce the 5 awardees and their research. These researchers span...Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and...discovery, and synthetic biology. Read on to learn more about the five awardees and their promising research projects... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...Expression Systems Synthetic Biology Resources Learn about Global Plasmid Distribution ...

  10. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...nano-lantern emitted yellow-green light and exhibited 3-5 fold increased brightness over previous BRET probes...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns...Nano-lanterns, Takai et al. continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL)... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...Additional Resources on the Addgene Blog Learn about Photoactivatable Fluorescent Proteins Check Out ...

  11. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into...antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening your cloning... plasmid is a great start when cloning, but how about being able to choose those that contain plasmid ... a widely used method to do just that! (To read about other screening strategies, check out our screening... called α-complementation. The system described above was put to practical use in the following way. Scientists...

  12. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...Using Virus in Your Research: A Primer for Beginners 5 Tips for Troubleshooting Viral Transductions Additional...conduct the screen. To help alleviate some of this labor for our users, we now provide the Brunello (targeting... hear from you if you have used or are thinking about using our viral services. Please send any questions...

  13. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ... 83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  14. Hot Plasmids - January 2023

    Type
    Blog Post
    ... via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w. Epub... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

  15. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    .... Cells should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how ...

  16. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...e23 . https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M,...the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids... availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley...mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has been able...clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. ...

  17. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...found that KillerRed required longer exposure times (5 minutes vs 10 seconds) to achieve the same level of... in culture and in vivo.  Read on to learn more about this killer illumination technique! What is a photosensitizer...within the near-infrared (NIR) spectrum (>620 nm). Above is a brief schematic of how the MG-2I-dL5** FAP-...use MG-2I-dL5** with your research? Let us know about your experiences using photosensitizers in the comments... to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on Addgene.org...

  18. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ... vectors through pseudotyping.” Curr. Gene. Ther. 5 (2005) 387-398. PubMed PMID: 16101513. PubMed Central...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

  19. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...Ishitani R, Zhang F, Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014...that screen with a system you are really confident about working for most genes and individual targeting ... system, Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could...RNA we use. What we have to do now is learn more about the rules.” He said that there is no theoretical...References & Further Reading: For more information about SAM, including a tool for getting optimized guide...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...insert. If the insert is large, we may only check the 5’ and 3’ ends to verify that the sequence assembly ...One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence...bp of clean sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature ...96-well microplates (identical copies, see image above). We then select forward and reverse primers for...specifically to target this region, as outlined above. Avoid primers that bind within or must read through...
Showing: 281 - 300 of 451 results