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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 539 results for: genome

Showing: 281 - 300 of 539 results

  1. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...William RA, et al. "Serine recombinases as tools for genome engineering." Methods 53.4 (2011): 372-379. PubMed...Blog Learn How Piggy Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from... Pages Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

  2. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...embryo derived-ES cells.They also conducted whole genome sequencing in selected control and CRISPR-Cas9 ...predicted off-target effects, they limited their whole genome sequencing to a small percentage of edited embryos...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    ...transcription. However, with scAAV the effective genome size usable is shortened (from ~4.8 kB [8] to ~...performance profiles and should be suitable for genome editing. These vectors can be used to improve targeted...adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of...

  5. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...generation packaging systems further split the viral genome by removing the viral Rev gene from the packaging...This system improves safety by dividing the viral genome into additional plasmids thereby increasing the...

  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...this protein must undergo as it interrogates your genome. If you are interested in experiencing Cas9 up ...Addgene Blog Learn All about CRISPR Use CRISPR for Genome Visualization Tips for a First Time CRISPR User...

  8. AAV ddPCR Titration

    Type
    Protocol
    ...AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents the physical number...

  11. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...be delivered to cells both transiently and for genomic integration. Lab-engineered viruses will package...package your genetic cargo in lieu of their own genomes and deposit this cargo upon host infection. Packaging...

  16. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we receive...: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read ...

  18. Call for Guest Bloggers

    Type
    Blog Post
    ... Biogen interested in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR...

  20. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...-stranded intermediate into the single-stranded genome that's then packaged into AAV’s protein shell, ...: AAV titering by qPCR quantifies the number of genome-containing viral particles that are present in ...
Showing: 281 - 300 of 539 results