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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 535 results for: genome

Showing: 281 - 300 of 535 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    ...transcription. However, with scAAV the effective genome size usable is shortened (from ~4.8 kB [8] to ~...performance profiles and should be suitable for genome editing. These vectors can be used to improve targeted...adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of...

  2. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...generation packaging systems further split the viral genome by removing the viral Rev gene from the packaging...This system improves safety by dividing the viral genome into additional plasmids thereby increasing the...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...this protein must undergo as it interrogates your genome. If you are interested in experiencing Cas9 up ...Addgene Blog Learn All about CRISPR Use CRISPR for Genome Visualization Tips for a First Time CRISPR User...

  7. AAV ddPCR Titration

    Type
    Protocol
    ...AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents the physical number...

  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...be delivered to cells both transiently and for genomic integration. Lab-engineered viruses will package...package your genetic cargo in lieu of their own genomes and deposit this cargo upon host infection. Packaging...

  13. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we receive...: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read ...

  15. Call for Guest Bloggers

    Type
    Blog Post
    ... Biogen interested in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR...

  16. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term ... it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral genomes (vg) per mouse. Figure 3 shows a gross comparison...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... sites, the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left...fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of organisms ...

  20. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...-stranded intermediate into the single-stranded genome that's then packaged into AAV’s protein shell, ...: AAV titering by qPCR quantifies the number of genome-containing viral particles that are present in ...
Showing: 281 - 300 of 535 results