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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 297 results for: hal.2

Showing: 281 - 297 of 297 results

  1. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next time is ...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...

  2. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

  3. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through how...

  4. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...

  5. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...) or the American Chemical Society (ACS) are just 2 examples of organizations that hold large, annual ...development (or lack thereof) One of the biggest challenges facing students today is that many graduate and...

  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....the power of lethal mutation co-selection. Only animals rescued for the conditional lethal mutation survive...selection. Knock-in events tend to be rare, and the challenge with any experiment is in efficient recovery of...co-selection approach relying on repair of a conditional-lethal mutation to identify edits with minimal screening...screening effort. Selecting for repair of a lethal mutation should remove much of the unedited “haystack”, ...viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted in efficiencies...Engineering of theCaenorhabditis elegansGenome with Lethal Mutation Co-Conversion and Inactivation of NHEJ...

  7. Finding Your Perfect Job After University

    Type
    Blog Post
    ...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

  8. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...community is active, engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation... revolutionized allelic exchange. Even now, only half our lab has switched over to pTOX. However, we feel...

  11. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...lack thereof): Information sharing gets more challenging the bigger an organization gets, and creating...

  12. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps are the best ...

  14. A Control for All Seasons

    Type
    Blog Post
    ...as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include...possible due to a lack of available materials, lethality of knockdown, or other factors, consider using...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...myriad cell types and subtypes, and a familiar challenge for anyone who has done histology on brain tissue...

  16. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...

  17. Molecular Biology Reference

    Type
    Guide
    ...indicated, the antibiotic powder can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions...for adding the next nucleotide, the reaction is halted once the fluorescently tagged base is incorporated...converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does not require...
Showing: 281 - 297 of 297 results