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...to a microfuge tube. Add 8 µL of PBS to a total volume of 13 µL . Prepare the recombinant antibody Dilute... issues. Plot the mg/mL versus the HC + LC Area column for the IgG standards in Google Sheets. Open exported...

...purity. Note: Purity is measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for...

... mostly absorbed (there is a very small visible volume of pooled liquid remaining on the surface). We ...

...concentrations. We recommend mixing 2μg each in a total volume of 50μL - add additional annealing buffer if necessary...

... of reagent that will be resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place...

...and vector to use for the ligation reaction. The volume of vector DNA and insert DNA used in the ligation...

...Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume 500 μL *Plasmid concentrations and ratios should...

...sample. To ensure that your sample is adequately illuminated, look through the eyepiece. Adjust the power ...