...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...

...Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene...on the antibodies. Nature 521:274–276. https://doi.org/10.1038/521274a  Bradbury A, Plückthun A (2015)...antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L...antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787  Taussig MJ, Fonseca... the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995  ...

...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...

... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying...sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps.....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...

...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...topics in their area of research. The society also organises PhD information events and summer internship information...support each other and provide advice. The society organizes numerous recreational and educational events throughout...for their students. Another large goal of the organisation is to encourage iGEM alumni to mentor younger...Bloggers Learn How to Get Involved in a Profession Organization ...

...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific... well. Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus...

...precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing...labeling Isaacman-Beck et al., bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for... Diessl et al., Microbial Cell, 2020. https://dx.doi.org/10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent... tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/10.1038/s41598-019-53722-0 ...

...and impact of your work. Please email deposit@addgene.org if you would like to contribute to the catalog...share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If you have suggestions...immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone, ME...Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury A, Plückthun...antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources We would also like to...

...Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito...reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585   Ding T, Huang C, Liang Z, Ma...Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner R (1980) ...National Academy of Sciences 77:4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank...efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118 Additional resources on ...

...are better for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred...involve enhancing optical live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for ...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc...extraordinary molecular detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable...mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during...Catch Up on Your GFP Background Resources at Addgene.org Find Photoactivatable FPs in Empty Backbones...

...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...visualization of FP attributes (which turned into www.fpvis.org).  That page has been very useful over the years... and dissemination of information about FPs. FPbase.org FPbase is a project written in Python & Django...(so feel free to moderate your own version of fpvis.org with the proteins of your choosing!), and they...Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent protein plasmids Find...

...official by registering BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re...local companies and BosLab has grown into an organization consisting of over twenty members and volunteers... involved, check out the local groups page on DIYBio.org to find a community lab in a city near you. To...Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville...Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! Find Free and Open...

...support. “It is an exciting and emotional time!” George Church tries to recruit people who are nice as ...“Treating people fairly does not mean equally.” George Church was surprised by “how much better it is ... to lead his team as a scientists and a friend. George Church runs his lab fairly similarly to his advisor... Or share your success stories below. And don't forget to stay tuned for the last post in this series ...Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the ...

... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other...in Cell and Developmental Biology 6: . https://doi.org/10.3389/fcell.2018.00054 Sander JD, Joung JK ...genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, ...of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722 Additional resources ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

...functional variable regions. mAbs 10:539–546. https://doi.org/10.1080/19420862.2018.1445456  Holzlöhner P, Hanack...Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832  Lipman NS, Jackson LR, Trudel LJ...Information Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron... Biological Chemistry 291:20993–21007. https://doi.org/10.1074/jbc.m116.736942  National Research Council...monoclonal antibodies for immunotherapy Resource on Addgene.org Check out Addgene’s antibody plasmid collection...

...GPCRome. Nat Struct Mol Biol 22:362–369 . https://doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP,...Proximity Labeling. Cell 169:338-349.e11 . https://doi.org/10.1016/j.cell.2017.03.028 Roth BL (2016) DREADDs...for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki...genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional resources...our quick guide to chemogenetics Resources on Addgene.org Check out Addgene's chemogenetics guide Find...

...monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating...by a lack of nutrients and targets cytosol and organelles to the vacuole/lysosome for degradation and recycling...more fine-grained detail about what proteins or organelles are being degraded) and it is slow. Alkaline...for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161...Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent...

...2020). https://pubs.acs.org/doi/10.1021/acscentsci.9b01093 More resources on addgene.org  View Addgene's...cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt.3290 Rozners, E. Chemical Modifications...Frontiers in Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu, Y., Wu,...Molecular Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori, E., Satyapertiwi...

...National Academy of Sciences 104:5163–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa J, Broek...addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128/mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré...monkeys. Eur J Neurosci 50:2801–2813 . https://doi.org/10.1111/ejn.14429 Kimple ME, Brill AL, Pasker RL...Current Protocols in Protein Science 73: . https://doi.org/10.1002/0471140864.ps0909s73 Additional references...the many types of protein tags References on Addgene.org Check out Addgene's Chemogenetics Guide Find...

... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check Out Our ...