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Showing: 281 - 300 of 948 results

  1. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...Analytical Chemistry, 83(12), 4327–4341. https://doi.org/10.1021/ac2010857  Sanger, F., Nicklen, S., & Coulson...Academy of Sciences, 74(12), 5463–5467. https://doi.org/10.1073/pnas.74.12.5463  Shendure, J., & Ji, H....Nature Biotechnology, 26(10), 1135–1145. https://doi.org/10.1038/nbt1486  Slatko, B. E., Gardner, A. F.,...Protocols in Molecular Biology, 122(1). https://doi.org/10.1002/cpmb.59  Wang, Y., Zhao, Y., Bollas, A....Nature Biotechnology, 39(11), 1348–1365. https://doi.org/10.1038/s41587-021-01108-x Additional Resources...Plasmid Quality Control Additional Resources on addgene.org Molecular Biology Reference Sequence Analyzer...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...do without it. So get yourself a good content organizer and build your own professional development etc...

  3. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman N. (2012)...Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/   Uhlen M, ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources on the ...The Basics of Western Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies...

  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin...PubMed PMID: 10470036. 3. Said, H. M. “Biotin: The forgotten vitamin.” American Journal of Clinical Nutrition...

  5. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...through your organization in this field. Please also enter the billing address for your organization. We will...customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! Today we’...you time. Anyone affiliated with the recipient organization can create an Addgene account and place an order... entered during checkout. At this time, your organization will have net 30 days to complete payment for...

  6. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

  7. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...These molecules occur naturally in bioluminescent organisms, but their real power lies in the clever ways ...Although humans have known about bioluminescent organisms and their characteristic radiance for some time...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first ...be responsible and began studying these marine organisms to find the culprits. He even obtained samples...

  8. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful!  I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption... Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of...

  9. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...as a free event! Organizing a science conference for the public How does one organize a public scientific...association with the Harvard graduate student organization Science in the News (SITN), we recently sponsored...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...opportunities to improve our communication and organizational skills provide great training for a number ...

  10. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even...knowledgeable staff is here to help. Contact us at mta@addgene.org or +1 617-225-9000, or check out our MTA reference...

  11. Finding Your Science Policy Path

    Type
    Blog Post
    ...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...

  12. Antibodies 101: Validation

    Type
    Blog Post
    ...Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene...on the antibodies. Nature 521:274–276. https://doi.org/10.1038/521274a  Bradbury A, Plückthun A (2015)...antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L...antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787  Taussig MJ, Fonseca... the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995  ...

  13. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying...sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps.....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...

  14. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596-023-00927-3 Klompe, S. E., Vo, P...integration. Nature, 571(7764), 219–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova...States of America, 114(35), E7358–E7366. https://doi.org/10.1073/pnas.1709035114 Witte, I. P., Lampe, G....transposase. Science, 388(6748), eadt5199. https://doi.org/10.1126/science.adt5199 Additional Resources on...Flexibility to CRISPR Editing Additional resources on Addgene.org Addgene's CRISPR Guide (Jump to Large Edits)...

  15. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...

  16. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...topics in their area of research. The society also organises PhD information events and summer internship information...support each other and provide advice. The society organizes numerous recreational and educational events throughout...for their students. Another large goal of the organisation is to encourage iGEM alumni to mentor younger...Bloggers Learn How to Get Involved in a Profession Organization ...

  17. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific... well. Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus...

  18. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing...labeling Isaacman-Beck et al., bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for... Diessl et al., Microbial Cell, 2020. https://dx.doi.org/10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent... tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/10.1038/s41598-019-53722-0 ...

  19. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...and impact of your work. Please email deposit@addgene.org if you would like to contribute to the catalog...share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If you have suggestions...immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone, ME...Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury A, Plückthun...antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources We would also like to...

  20. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito...reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585   Ding T, Huang C, Liang Z, Ma...Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner R (1980) ...National Academy of Sciences 77:4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank...efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118 Additional resources on ...
Showing: 281 - 300 of 948 results