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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 291 results for: poly

Showing: 281 - 291 of 291 results

  1. Molecular Biology Reference

    Type
    Guide
    ...DNA polymerase adding nucleotides. The major difference in this process occurs when the polymerase incorporates.... During replication, DNA unwinds and the DNA polymerase enzyme binds to and migrates down the single ...replication requires the 4 nucleotides, a DNA polymerase enzyme, the template DNA to be copied, and a ... DNA and acts as a starting point for the DNA polymerase. Thus to replicate a piece of DNA in vitro one...

  2. Protocol - How to Design Primers

    Type
    Protocol
    ...Primers How to Design a Primer You may also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...transcription started by 5' LTR by the addition of a polyA tract just after the R sequence. WPRE in cis Woodchuck...efficient viral packaging. cPPT in cis Central polypurine tract; recognition site for proviral DNA synthesis...

  4. Gibson Assembly Protocol

    Type
    Protocol
    ...anneal to each other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...transcription started by 5' LTR by the addition of a polyA tract just after the R sequence. WPRE in cis Woodchuck...efficient viral packaging. cPPT in cis Central polypurine tract; recognition site for proviral DNA synthesis...

  9. Kit Free RNA Extraction

    Type
    Protocol
    ...RNase free tubes: microcentrifuge tubes, 4 mL polypropylene tubes RNase decontamination solution like RNase...

  10. Adenovirus Guide

    Type
    Guide
    ...vector capsid. Icosahedral Having the shape of a polyhedron with 20 faces, each being an equilateral triangle...

  11. CRISPR Guide

    Type
    Guide
    ...159 (3), 647–661. PMID: 25307932 Hilton, I. B., D’Ippolito, A. M., Vockley, C. M., Thakore, P. I., Crawford...
Showing: 281 - 291 of 291 results