We narrowed to 303 results for: rigi
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TypeBlog Post...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...
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Plasmids 101: Using Transposons in the Lab
TypeBlog Post...Tol2 or miniTol2 is a truncated version of the original Tol2 and has ~3-fold increase in transposition... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...their undergraduate years. We each have a unique “origin story,” but taking the first step into the world... -
Transferable Skills Guide: Teamwork
TypeBlog Post...Next Great Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...Gautier et al., 2008). Although based on the same original enzyme, SNAP-tag and CLIP-tag show good specificity... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...use, including all information provided in the original reference library, as well as GC%, reverse compliment... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...especially immune cells and cells of epithelial origin. Suppressing function, creating tolerance, and ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...nanoparticles, maintaining intensity at ~85% of its original value after 60 minutes of illumination, highlighting... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...specific to the lineage of the target cell in the original somatic cells [41]. The in vivo and in vitro methods... -
Delivery Methods for Generating iPSCs
TypeBlog Post...EBNA1) based episomes: These plasmids carry the origin of replication (oriP) element and the cis-acting... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...adenine base editing (TadCABE) This post was originally written by Mary Gearing in August 2016 and was... -
Technical Design of a Western Blot
TypeBlog Post...validation data, or follow the references to find the original publication and ensure that it includes validation... -
Using AAV for Neuronal Tracing
TypeBlog Post...methods such as electrophysiological recordings. The origins of these methods are naturally occurring viruses... -
Molecular Biology Reference
TypeGuide...Biology Reference Jump To: Molecular Biology Basics Origins of Molecular Genetics Plasmid Elements Working ...Genetic Code Webpage and Blog References Protocols Origins of Molecular Genetics The concept of genes as carriers... this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a region in...parental cell, and are thus termed clones of the original cell. The plasmid DNA is similarly referred to...their simplest form, plasmids require a bacterial origin of replication ( ori ), an antibiotic-resistance...common plasmid elements: Plasmid Element Description Origin of Replication ( ori ) DNA sequence which directs... cultured human cells. Although plasmids were originally used to understand protein coding gene function... -
Sequencing Primers
TypeGuide...reverse primer F1ori-F GTGGACTCTTGTTCCAAACTGG F1 origin, forward primer GAL1 AATATACCTCTATACTTTAACGTC (...forward primer pACYC-F TGAAGTCAGCCCCATACGAT p15A origin, forward primer pAd-CMV GCTAGAGATCTGGTACCGTC For...vector pBR322ori-F GGGAAACGCCTGGTATCTTT pBRS322 origin, forward primer pBRforBam CTTGGAGCCACTATCGAC In... primer pGP704-R AACAAGCCAGGGATGTAACG R6K gamma origin, 3' of MCS in pGP704 vector, reverse primer pHybLex...primer SV40pro-F TATTTATGCAGAGGCCGAGG SV40 promoter/origin, forward primer SV40-spliceR CACAAAGATCCGGACCAAAG... -
Modular Cloning Guide
TypeGuide... K. phaffii ). GoldenPiCS Kit Yeast Expression Brigitte Gasser , Diethard Mattanovich , and Michael Sauer... and Cultivarium Tools 189 plasmids, including origins of replication and selection markers for a variety...Kit Genome Engineering, CRISPR, Yeast Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated... -
CRISPR Guide
TypeGuide...target sequence present in the gRNA. CRISPR was originally employed to knock out target genes in various..., be sure to check the library information and original publication to see if a library is suitable for... of your amplified library matches that of the original library. Next, you’ll need to generate lentivirus... -
Antibody Guide
TypeGuide... and can either be used while suspended in the original media or isolated and placed in a buffer. Antibody...antibodies can distinguish between different species of origin, isotypes, and isotype subclasses of a primary ...