We narrowed to 541 results for: SHI
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TypeBlog Post...examines the inherent brokenness of scientific publishing and research funding. I’ve been aware of these... place. Can we address problems in scientific publishing without acknowledging the systemic sexism in ...
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Great Results Start with Great Standard Curves
TypeBlog Post...fit and 0 indicating that there is no linear relationship between the observed and predicted values. This... is no correlation or it could mean that the relationship is non-linear and you should use a different... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...maintaining the structure of your samples, the relationships between cellular components, and the accessibility...saponin. However, if you are not worried about washing away your target (for example, some cytoskeletal... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post... they looked for a biological explanation, a relationship between the genetic dependencies they identified... ancestries. While there were some exciting relationships identified, 48% were found to be artificial,... -
Lentiviral Vector Uses and Overview
TypeBlog Post...self-inactivating lentivirus vector. Miyoshi H, Blömer U, Takahashi M, Gage FH, Verma IM. J Virol. 1998... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post...budget narratives, logic models, established partnerships and collaborations, and commitments to and proof...close-out a grant. Grant professionals also build relationships with foundations and government grantmakers.... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...Addgene takes care of everything including MTA and shipping. Addgene realizes the One-Stop shopping for a ...short period of time and may need to do some fashionable projects. However, I hope that they also have... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post... If you are miserable at home or work, your relationships will suffer in both the short run and the long...agree on before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...s41586-019-0908-x Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post... typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas campestris ...10.1111/1574-6968.12241 Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved broad-host-range... -
Bioinformatics at Addgene
TypeBlog Post...and how our search for solutions led us to a partnership with Seqera Labs. DNA sequencing...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than... -
Transferable Skills Guide: Creativity
TypeBlog Post...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face... -
Hot Plasmids: Fall 2024
TypeBlog Post...Antibody here! Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior in rats in the forced...neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior. There ... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...mouse an attractive tool to probe gene-function relationships in defined substructures of the CNS as well ...Rochester Medical Center. He studies gene-function relationships in the CNS using CRISPRa as a member of the ... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...represents the proportion of cells that have either a frameshift or 21+ bp indel. This score is a useful measure...Represents the proportion of cells that have either a frameshift or 21+ bp indel. This score is a useful measure... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...difficult to treat in the future. Patients not finishing the whole course of antibiotics prescribed to ... cleanliness and personal hygiene standards. Handwashing is an essential component of preventing infection... -
CRISPR Activation: A Practical Guide
TypeBlog Post...OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional...Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens. Cell Systems... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...of North Carolina at Chapel Hill. Under the co-mentorship Blossom Damania and Jack Griffith, her research... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...Multiple uses: From multiplexing to multiscaling NIR-shifted spectrum is perfect for use in spectral multiplexing...autofluorescence, low scattering, and deep penetration of red-shifted light. The same NIR FPs and biosensors can be imaged...