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  3. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...Grant_Chat    Additional Resources Read other Career Posts on the Addgene Blog! ...

  4. Plasmids 101: Protein tags

    Type
    Blog Post
    ... list more common tags than are described in the post, but have been categorized to help you better assess...Healthcare Life Sciences. Addgene pages and blog posts of interest: Which Fluorescent Protein Should I...

  8. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...viral vector services and resources!   This blog post was written by Brook Pyhtila and Brian O'Neill.  ...

  11. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...sites! In this post, we'll cover Cas9 multiplexing options, but also check out our blog post about multiplexing... information on this system, check out this blog post.  Multiplexing in zebrafish Wenbiao Chen Lab Golden...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...having issues, you’re in the right place! In this post, we’ll talk about western blot optimization and ... on your gel or blot, Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel ... and 2 in our Technical Design of a Western Blot post to make sure you have the optimal gel for your experiment...consider (see our “technical design of a western blot”) post, most imaging issues can be fixed real-time by exposing... Splice variants Check literature Posttranslational modifications Check literature Highly...

  13. New Viral Vectors - March 2024

    Type
    Blog Post
    ...ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 21 additions we've made to our ...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...proximity to the region to be edited. In this blog post, we will cover three ways to circumvent this limitation...and Mary Gearing contributed to the update of this post. References Abudayyeh OO, Gootenberg JS, Essletzbichler...

  18. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...visualization methods. Stay tuned for a future blog post to learn more about these techniques!  Direct vs...effective and reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...

  19. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... or detail. By the end of 2023, we had over 900 posts, which were read by researchers all over the world...mean all over (Figure 3). We continue to add new posts every week, and we’ve made our popular blog series...

  21. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...This post was contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful...between loxP sites (For details see Cre-lox blog post). Optimizing FLP FRT through smart engineering Different...markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in high concentrations...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

  22. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...relationships you develop will lead! Read our blog post about fostering communication in remote collaborations...Blog Check out Our Career Topic Page Read Other Posts in the Transferable Skills Guide Developing Transferable...keeping track of speakers. During your graduate or postdoctoral work there are loads of opportunities to create...collaborative environment. Think of student groups, PostDoc associations and specific projects where you share...

  23. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...Out Our Networking for Scientists Blog Posts Read Our Career Posts Read Joanne's Management for Scientist's...frame.   I suggest that all graduate students and postdocs form peer mentoring groups to practice giving ...

  24. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...Last updated Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle...different types of Cas9 activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less ...

  25. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Another factor to consider is the age of your culture post thaw. In general, the higher the passage number ...produce the virus, titer the virus (read this blog post for tips on titering your lentiviral prep!) and ...

  28. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... major ones in Addgene’s repository later in the post. Advantages of prime editing Less constrained by...treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration of...plasmids! Find plasmids from David Liu's lab! This post was originally written by Jennifer Tsang in October...
Showing: 451 - 480 of 783 results