Addgene

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Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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Blog Post

...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...
Simplify Cloning with in vivo Assembly

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Blog Post

...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

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Blog Post

...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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Blog Post

...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...
Plasmids 101: Colony PCR

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Blog Post

...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...
A Career in Grants: How To Become a Grant Professional

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Blog Post

...Becoming a grant professional is rewarding and challenging! Jo Miller, GPC, CSMS is the founder and principal...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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Blog Post

...that provides a community for biologists and bioengineers to share their educational materials, protocols...
Your Lentiviral Plasmid FAQs Answered

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Blog Post

...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...
Negotiating Work and Life: How to Find the Joy

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Blog Post

...your plan to live a good life may be the most challenging assay you will ever have the opportunity to develop...
Fluorescent Proteins 101: Aptamer Fluorophores

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Blog Post

.... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...
How to Write a Scientific Review Article

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Blog Post

...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...
Mesothelioma - Causes, Symptoms, and Treatment

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Blog Post

...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...
Antibiotic Resistance: An Old Solution but a New Problem

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Blog Post

...flu) Overuse of antibiotics in livestock farming Lengthy courses of treatment with antibiotics These scenarios...
Twenty Years of Sharing: Expanding Accessibility

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Blog Post

...countries means that our shipping team is always engaged in some form of problem-solving. When universal...
Hot Plasmids: Fall 2024

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Blog Post

...common microscopy methods. Plus, the luminescence wavelength (460 nm) is shorter than many popular fluorescence...
Hot Plasmids Spring 2024

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Blog Post

...This is similar to the proposed mechanism for engineered pegRNAs (epegRNAs), which add a structural motif...
Tips for Writing a Good Cover Letter

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Blog Post

...skills and experiences fit the job requirements, strengthened an application. Even so, there were examples...
Plasmids 101: Broad Host Range Plasmids

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Blog Post

...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...
Transferable Skills Guide: External Collaborations

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Blog Post

...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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Blog Post

...Induced Pluripotent Stem Cells (iPSCs): Benefits, Challenges and the Road Ahead." Journal of Alzheimer's disease...
AAV Titers: Where Do They Come From And What Do They Mean?

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Blog Post

...28546538. PubMed Central PMCID: PMC5445070. 6. Wang, Feng, et al. "A reliable and feasible qPCR strategy for...
Transferable Skills Guide: Career Planning Resources

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Blog Post

...development (or lack thereof) One of the biggest challenges facing students today is that many graduate and...
Plasmids 101: Plasmid Incompatibility

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Blog Post

....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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Blog Post

...Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa. Financial and moral supporters Denise Akob, ...
Plasmids 101: Codon usage bias

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Blog Post

...‐gene study reveals a high success rate of full‐length human proteins expressed in Escherichia coli." ...
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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Blog Post

...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity...
New Tools Enable CRISPRa for Neuroscience Applications

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Blog Post

...uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to...
To Codon Optimize or Not: That is the Question

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Blog Post

...optimization? Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous...
Getting the Most from Your Lentiviral Transduction

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Blog Post

...envelop protein. Many times lentiviral vectors are engineered with non-native envelopes, a process called pseudotyping...
Plasmids 101: The Promoter Region – Let's Go!

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Blog Post

...alcohol dehydrogenase I Repressed by ethanol Full length version is strong with high expression. Truncated...
March for Science

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Blog Post

...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold...
Antibodies 101: Beyond Surface Labeling

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Blog Post

...population can be seen as a stepwise decrease in signal strength in a flow plot. d = days post treatment. Created...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

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Blog Post

...priorities in mind, many researchers go to great lengths to keep accurate records, but given the complexities...
Tips for Getting a Faculty Position

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Blog Post

...temptation to provide too many details. Reverse engineer your talk. Identify the key points you want to...
Filming Science Videos in the Age of Social Distancing

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Blog Post

...small operation, so sometimes it can be a big challenge. But ask any filmmaker or videographer and they...
Plasmids 101: Biotinylation

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Blog Post

...2003): 8074–8075. PubMed PMID:12837056. 8. Pan, J. feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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Blog Post

... a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence specificity...
Thoughts on the Future of Research 2015 Boston Symposium

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Blog Post

...received a doctoral degree (or equivalent) and is engaged in a temporary and defined period of mentored advanced...
How to Negotiate a Successful Lab Start Up Budget

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Blog Post

...success. Therefore, it only makes sense to reverse engineer your budgetary needs from your experimental goals...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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Blog Post

...goals from effecting our experiments. How do we engender better experimental design? The summation of many...
Antibodies 101: Multiplex Immunofluorescence

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Blog Post

... Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG...
Antibodies 101: The Basics of Western Blotting

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Blog Post

...A stacking gel typically has a different ionic strength and lower pH and acrylamide content than the resolving...
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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Blog Post

....2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An ...
A Guide to Getting Started in Undergrad Research

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...lab Writes a lot of grants Varying degrees of engagement with what happens in the lab Official title is...
Which Fluorescence Microscopy Techniques is Best for Me?

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Blog Post

...model embryos >15 μm Thsee are some of the most challenging samples to image as they require both optical...
How to Deposit Your Plasmids with Addgene

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Blog Post

...requested through Addgene. For example, you could enter "Feng Zhang (Addgene plasmid #52961)". The vector type...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

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Blog Post

...directly into a different cell type, bypassing the lengthy processes of reprogramming. The process was first...
Deep Dive: qPCR

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Blog Post

...channels - helpful if you forget to set the channel wavelength before you run the plate. Analyzing There...
Educational Resources

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Guide

...molecular biology techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid...
Promoters

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Guide

...into a protein. RNA polymerase II transcribes messenger RNA (mRNA) which is the RNA responsible for providing...

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