We narrowed to 881 results for: NSI

1. CRISPR Challenges: Standardization and Homology Directed Repair

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   Blog Post

   ...Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently leads to high on-target editing and low off-...

2. In Vivo Biotinylation of Bacterial Fusion Proteins

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   ...kinetics of the target ligand. Another important consideration is whether the affinity of an experimental protein-ligand...

3. The Golden Stopwatch Award: Recognizing Facilitators of Research

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   Blog Post

   ...quickly! If your TTO receives a Golden Stopwatch, consider sending an email to thank them--after all, they...

4. Single-cell tracking of lineage and identity with CellTag

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   ...labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6 bp fixed region...

5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   ...of fusions and FRET reporters. mTFP1 is also insensitive to physiologically relevant changes in pH. Find...

6. New and Upcoming Viral Vectors - Spring 2019

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   ...calcium sensors are single-wavelength sensors with intensities that change in response to calcium binding. There...

7. New Viral Vectors - Winter 2025

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   ... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers...

8. Viral Vectors 101: Transductions

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   ...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...

9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   ...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...

10. Antibodies 101: Epitope Tags

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    ...detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...

11. Summer SciComm: Science Storytelling

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    ...need to be overcome. Conflict, and its inherent tension, is interesting. It’s what drives a story forward...

12. RUBY-Red Siliques

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    ...and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under the same...

13. Antibodies 101: Epitope Availability

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    ...looking for a protein of interest while it’s in a transition state or specific configuration. So how do you...

14. Hot Plasmids and Viral Preps - September 2021

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    .... These plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE...

15. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    ...directly conjugated primary antibodies can be expensive and may not provide enough signal to visualize...

16. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ...Virus structure While all viruses have a genome inside a protein shell known as a capsid, some also have...

17. New and Upcoming Viral Vectors - December 2019

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    ...in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials, is now offered ...

18. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    ...natural or synthetic, the researchers looked at intrinsic properties (GC content or rare codon percentage...

19. The Strength of Story Telling

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    ...project and contributions, rather than getting defensive or getting into ‘he said; she said’. We spoke ...

20. Plasmids 101: TOPO Cloning

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    ...hydroxyl group! You may want to include extra extension time after the last cycle of PCR to make sure ...

21. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    ...HDR, which could lower HDR efficiency. Higher sensitivity to off-targets More research is needed to compare...

22. Plasmid Cloning by PCR

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    ...   The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base pairs on the ...

23. Addgene's Top 10 Blog Posts of 2015

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    ...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...

24. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    ...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

25. With Our New Viral Service, We're Taking CRISPR Further!

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    ...requested and incredibly useful tools, they require extensive background work from the requesting scientist ...

26. New and Upcoming Viral Vectors - June 2019

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    ...to the study of neuroscience. To this end, new biosensing AAV are always being developed and we are excited...

27. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ...fluorescent proteins under the control of promoters responsive to water or nutrient levels can help him determine...

28. Visualizing Genomic Loci with CRISPR-Sirius

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    ...pairs were easily visualized and results were consistent with copy number variation (CNV) analysis of ...

29. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    ...bedside is, in addition to thorough ethical considerations, in making sure that the research has been ...

30. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    ...physical location didn’t matter. I didn’t have to consider that aspect and I could plan more with regard ...

31. Scientists Map the SARS-CoV-2-Human Interaction Network

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    ...published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in...

32. Plasmids 101: Gram Negative and Positive Bacteria

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    ...into a specific bacterium, it can be important to consider the type of bacteria it is being used in, and ...

33. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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    ...we call “asymmetric collaborations.” These are transient partnerships where a researcher makes a small ...

34. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ...3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...

35. Viral Vectors 101: Inverted Terminal Repeats

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    ... they flank the gene of interest. A single ITR consists of palindromic arms (A-A’, B-B’, and C-C’) that...

36. Plasmids 101: NGS Quality Control for Pooled Libraries

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    ...post has helped clarify some of the important considerations when using, amplifying, and verifying pooled...

37. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    ...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of...

38. Addgene Welcomes John Doench to the Advisory Board

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    ... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

39. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...proteins to be imaged together, you will need to consider the same factors as when choosing an individual...

40. Advice for Scientists Starting a Lab

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    ...decide on the best way to structure roles and responsibilities as we grew.” Initially, he spent the majority...

41. OCaMP: A New Calcium Indicator for Neural Imaging

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    ...Resources References Aggarwal, A, et al. (2025). A sensitive orange fluorescent calcium ion indicator for imaging...

42. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ...function of immune cells that are not excitable but responsive to calcium. We’d love to expand our cancer and...

43. Improved Plasmid Maps Powered by SnapGene

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    ...backing of SnapGene’s sequence viewer software and extensive feature library, our updated plasmid and sequence...

44. Screening for Successful Genome Editing with Digital PCR

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    ...commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...

45. Communicating Your Science With Help From ComSciCon

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    ...succeed in communicating. Even the most rigorous, insightful, and novel scientific research will be wasted...

46. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ...RNAi podcast segment RNA interference (RNAi) is extensively used in C. elegans research to study gene function...

47. Antibodies 101: Flow Cytometry Controls

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    ...unsure what amount of antibody to use, you should consider using an isotype control. Pro tip! Many companies...

48. A Quick Guide to a Career in Software Product Management

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    ...alone. A few years ago when I started my career transition from bench science to software product management...

49. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ...Please note that NGS verification is more time-intensive than Sanger sequencing or the other techniques...

50. New and Upcoming Viral Vectors - May 2020

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    ...lox sites. This system takes advantage of the extensive supply of transgenic reporter lines that supply...