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We narrowed to 569 results for: CIT

Showing: 301 - 320 of 569 results

  2. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... make your ideas easier to understand and more exciting. Practice presenting your chalk talk Perfect your...research program. You should be proud of it and be excited about getting the opportunity to realize your vision...

  3. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...nonprofits, who tend to prioritize candidates that are excited about their work and mission.  How do I know what...you excel in the role. It can also convey your excitement for that particular job, your commitment to the...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ...development, transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC...

  5. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...Labor issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed raising ...level. Not only is this in direct contradiction to explicit policies set up other institutions (like University...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... for a specific subset of RNAs. To gain this specificity, the eukaryotic RNAP can recognize and bind to... future post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type ...

  8. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to ...powerful boost to genome editing experiments. We’re excited to see how you continue to use and develop AAVs...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...hypothesized that genes that had evolved specifically for toxicity would allow stronger counter-selection than one...an efficient allelic exchange system, he is so excited to use it to explore how hospital-associated Gram-negative...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Proteins can also be biotinylated by exploiting the specificity of the E.coli BPL, BirA. If you recall, BirA ...post-translational modification of exceptional specificity.” Trends in Biochemical Sciences, 24.9 (1999b...

  11. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or break your...

  12. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...and the fluorescence signal weakens. (b) The heterogenicity of proliferative behavior in a cell population...

  14. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Attendees will include grad students (that are solicited for feedback), possibly the Dean, and even some...decks leads to the opposite and can suffocate an exciting idea in a swamp of unnecessary details. You DO...

  15. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Overall, the synthetic rAbs demonstrate remarkable specificity for their targeted integrin subtype, even when... with the Springer lab, reported the binding specificity, kinetics, and affinity of these 11 newly discovered...

  16. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... Research in Madison, Wisconsin. It is commonly cited that approximately one-third of cellular proteins...the reaction, or have difficulties with kinase specificity in vitro. The establishment of a system in which...

  17. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...skipping approach in mice, DMD researchers are very excited. This approach may also be applicable to a number...the body, especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized...

  18. Optogenetics Guide

    Type
    Guide
    ...Chlamydomonas reinhardtii . Channelrhodopsin-1 (ChR1) is excited by blue light and permits nonspecific cation influx...physiological pH and trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic ... as light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements...are some key factors to consider: Optogenetic excitation or optogenetic inhibition. First things first...experiment? Depending on your answer, you’d pick an excitatory or inhibitory opsin, respectively. Color of activation... EJ, Tian Z, et al. 2014. Independent optical excitation of distinct neural populations. Nat Methods. ...Finkelstein J, Paz JT, et al. 2011. Neocortical excitation/inhibition balance in information processing ...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9... in order to call that gene a hit.  This is an exciting time for functional genomics, with an ever-expanding...

  20. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... occur. The RNA sequence and PAM site confer specificity of the nuclease; however, many Cas and Cas-like...Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol...
Showing: 301 - 320 of 569 results