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We narrowed to 369 results for: Cre expression vector

Showing: 301 - 320 of 369 results

  1. Neurodegeneration Research Collection

    Type
    Collection
    ...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of aggregation-prone...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New...Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al. Nature. 2014 Apr 17...Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, ...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression HcRed1-N1 - Mammalian Expression HcRed1-C1 - Mammalian Expression dKatushka 588 635 22...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...pEiLOV-N1 - Mammalian Expression pGEX iLOV - Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1...

  3. CRISPR References and Information

    Type
    Collection
    ...cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...

  4. Antibody Plasmid Collection

    Type
    Collection
    ... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection of recombinant.... Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem...problem of in-frame amber codons in scFvs A new expression vector facilitating production and functional analysis...recombinant antibodies, created by cloning antibody components into plasmids and expressing these in bacteria...plasmid collections that can be used to create and express plasmid-based antibodies. Description Article... trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large...

  5. Plant Plasmids and Resources

    Type
    Collection
    ...designed for creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene...Parniske. GreenGate Cloning System to create plant expression vectors containing several cassettes and generate...maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes - Qi-Jun Chen...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on... - Tools for gene and protein expression and mapping of gene expression in plants. Figshare - Plant Illustrations... that drive general and species-specific gene expression (see the column ‘promoter’ in the tables for ...CRISPR Resources for a full selection of plasmids expressing different functional variants of Cas9 and empty...

  6. Plan Your Experiment

    Type
    Collection
    ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...AAV vectors offer some advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

  7. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...in-frame to these vectors. Plasmid...heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric FokI domain. Miller et...

  8. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...for many molecular biology protocols, including vector-insert joining for recombinant plasmid construction..."Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog...reliably detect single-base differences. The original creators of this technique visualized the ligation product...quencher pairs or qPCR-based methods to greatly increase the sensitivity of detection (14-20). LDR has ... 27). This methodology has been used to rapidly screen buffer conditions for Taq DNA Ligase and their ...electrophoresis (CE) (see below). This method allowed screening of all possible base combinations (Watson-Crick...of a 96-well plate, allowing 6 conditions to be screened per plate. The results indicated that the optimal...

  9. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...cell type-specific expression. The Allen Institute for Brain Sciences has screened thousands of enhancers...Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2...Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2...Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT Isocortex 223974 pAAV-AiE0050m...224056 pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_IT Isocortex 224038 ...220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_IT Isocortex 224043 ...220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_IT Isocortex 214522 ...

  10. Plasmids for Stem Cell Research

    Type
    Collection
    ...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 ...transdifferentiation utilize plasmids to increase expression of key factors. The tables below will help...iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and...

  11. Biosensor AAV Preps

    Type
    Collection
    ...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent... none Cre dependent 1, 9 Kim , GENIE 100839 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 CAG GCaMP6m none Cre dependent...

  12. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools because...non-dividing cells. These virusal vectors enable long-term stable gene expression and are permanently integrated...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ...immunogencity. These viral vectors can direct long-term transgene expression, but generally don't integrate... Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to...

  13. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Publication Empty gRNA Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to search... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...plasmids have been designed for use in mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...

  14. Microbiology Resources

    Type
    Collection
    ...Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma and... E. coli expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier...- Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc - Lindquist...Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources : Find...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...Santalucia Labs pCri System : Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth...constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each of the predicted open...

  15. Lentiviral Prep Service

    Type
    Collection
    ...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral Service...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus...52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused ...61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti...

  16. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...

  17. Depositor Collections

    Type
    Collection
    ...Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort Gene Vector Core Viral Vectors - Oka...research, visualization, protein expression, synthetic biology, and viral vector plasmids. Plasmid...to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant phosphoproteins...Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase...FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

  18. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  19. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  20. Structural Genomics Consortium Plasmids

    Type
    Collection
    ... Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with...cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine...SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids (empty backbones/controls): ID Plasmid...Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR ...hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: http://www.thesgc.org...
Showing: 301 - 320 of 369 results