We narrowed to 342 results for: GAI

1. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   ...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded...

2. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   ...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent...

3. Transferable Skills Guide: Conflict Resolution

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   Blog Post

   ...accomplish, but there is a potential for long-term gains when you can do it. If Party X proposes solution...

4. Filming Science Videos in the Age of Social Distancing

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   Blog Post

   ...what works and what doesn’t, and trying all over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn...

5. Antibodies 101: Normalization and Loading Controls for Western Blots

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   Blog Post

   ...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...

6. Tips for Getting a Faculty Position

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   Blog Post

   ...create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"...

7. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   ... populations with basically a smear in between. Again, it can depend on the type of stain whether a tighter...

8. An Integrin Antibody Toolkit from IPI

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   Blog Post

   ...αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived...

9. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   Blog Post

   ...template that corrects the blocking mutation and again identify the right clone from a few hundred. From...

10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...in the same orientation as those in the plasmid. Again, if they are in the opposite direction, both strands...

11. Evolution of Lab Techniques

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    Blog Post

    ... map DNA and examine genomes (6). The knowledge gained through these experiments allowed researchers to...

12. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    ...options in the deposit process in 2014 because of its gain in popularity. Gibson assembly is well known for...

13. CRISPR Guide

    Type

    Guide

    ...systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family members... 35301321 Edraki, A., Mir, A., Ibraheim, R., Gainetdinov, I., Yoon, Y., Song, C., Cao, Y., Gallant, J....

14. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ...signal in these assays (Dempster et al., 2019). Again, the experimental strategy is clear: for any gene...

15. Antibodies 101: The Basics of Western Blotting

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    Blog Post

    ...blocked, it is stained with a primary antibody against your protein of interest. But which antibody should...

16. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    Blog Post

    ...directly by creating well characterised antibodies against all human extracellular proteins with community-based...

17. Hot Plasmids: Spring 2025

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    Blog Post

    ... set of highly-specific recombinant antibodies against three of the six human glypican proteins (GPC1,...

18. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post

    ...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...

19. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...of whole phages as therapeutics, which is also gaining a lot of new traction lately (1, 2), depends on...

20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...