We narrowed to 344 results for: GAI

1. The Challenges of Cell Culture

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   Blog Post

   ...He studied biochemistry at Warwick University, gaining extensive cell culture research experience through...

2. Plasmids 101: The Promoter Region – Let's Go!

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   ...each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic RNAP can recognize...

3. Transferable Skills Guide: Conflict Resolution

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   ...accomplish, but there is a potential for long-term gains when you can do it. If Party X proposes solution...

4. Filming Science Videos in the Age of Social Distancing

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   Blog Post

   ...what works and what doesn’t, and trying all over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn...

5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   ...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent...

6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...in the same orientation as those in the plasmid. Again, if they are in the opposite direction, both strands...

7. Tips for Getting a Faculty Position

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   ...create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"...

8. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...

9. Antibodies 101: Introduction to Gating in Flow Cytometry

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   ... populations with basically a smear in between. Again, it can depend on the type of stain whether a tighter...

10. An Integrin Antibody Toolkit from IPI

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    ...αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived...

11. Evolution of Lab Techniques

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    ... map DNA and examine genomes (6). The knowledge gained through these experiments allowed researchers to...

12. Hot Biosensors 2022: Year-End Roundup

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    ...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded...

13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...template that corrects the blocking mutation and again identify the right clone from a few hundred. From...

14. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...options in the deposit process in 2014 because of its gain in popularity. Gibson assembly is well known for...

15. How to Design Your gRNA for CRISPR Genome Editing

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    ...signal in these assays (Dempster et al., 2019). Again, the experimental strategy is clear: for any gene...

16. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...directly by creating well characterised antibodies against all human extracellular proteins with community-based...

17. Technologies Enabled by NanoLuc® Luciferase

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    ...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...

18. Hot Plasmids: Spring 2025

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    ... set of highly-specific recombinant antibodies against three of the six human glypican proteins (GPC1,...

19. Technique: Probe Phage Genomes for Host Binding Proteins

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    ...of whole phages as therapeutics, which is also gaining a lot of new traction lately (1, 2), depends on...

20. Antibodies 101: The Basics of Western Blotting

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    ...blocked, it is stained with a primary antibody against your protein of interest. But which antibody should...