We narrowed to 402 results for: Mut
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TypeBlog Post... promoter with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus...
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Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post... fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings of the... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...damage other cellular structures, and genetic mutations are not easily reversible. Late last year, the... -
Five Popular Model Organisms, Part 2
TypeBlog Post...molecular tools, such as gene manipulation and mutagenesis techniques, are available to the scientific community... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...expression in strains of bacteria that are redox mutants so they have a more oxidizing cytoplasm. expression... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post... to knock down genes with potentially harmful mutations in therapeutic settings. Silencing of a target... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...process makes it easy to generate wild type and mutant constructs at the same time, rather than sequentially... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...molecularly evolved from mNeptune2 through 33 mutations and 2 deletions. CyOFP1 has a higher quantum yield... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and therefore should have reduced frequencies... -
Hot Plasmids: Spring 2025
TypeBlog Post...designed and screened a large library of iGluSnFR3 mutants, identifying the two best-performing variants ... -
Finding Your Perfect Job After University
TypeBlog Post...outcome for a specific combination of genetic mutations. This is a particular challenge in pancreatic ... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...gene is also useful in case the gene has a weird mutation that you didn’t know about (which could affect... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...samples are different from each other, or if every permutation of pairs within the five is different. To identify... -
Transferable Skills Guide: Teamwork
TypeBlog Post...year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone crazy. As it... -
Rett Syndrome
TypeCollection...caused from loss-of-function mutations in the MECP2 gene. Causative mutations for Rett syndrome typically...within patients with the same mutation, the location and type of MECP2 mutation can be a strong predictor ...missense mutations located in the MBD, and truncations prior to the NID are more severe than mutations further...to their laboratory website. Cell Line Mutation (DNA) Mutation (protein) Sex Source of Material Publication...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...meet diagnostic criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2... expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these X inactivation ratios ... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in your repair... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...the Vancouver SciCATs. Follow her on twitter @Point_Mutation, and @SciCATSyvr. Additional Resources on ...