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We narrowed to 682 results for: RON

Showing: 301 - 320 of 682 results

  1. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors...online content for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs...Plan Learn a Little about The Academic Labor Environment from the Future of Research Symposium Resources...

  2. Hot Plasmids - October 2020

    Type
    Blog Post
    ...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...genetically encoded fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA display (FingRs...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

  3. Engaging with science and society at pgEd

    Type
    Blog Post
    ... precision medicine research. A willingness to confront these tough issues is essential for building a...careers should take advantage of their academic environment and attend seminars, symposia, or courses in ...communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ...

  4. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ... challenge with using AdV is that it triggers a strong immune response after in vivo administration, which...loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes them ideal...identified, re-transform into a bacterial strain not prone to recombination for amplification. The final purified...

  5. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...fluorescent proteins, has led to the alternative acronym Fluorescence Resonance Energy Transfer. Unlike ...and acceptor is sensitive to a change in the environment which alters the FRET efficiency. Detecting certain...molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find...

  6. 6 Tips for Grant Writing

    Type
    Blog Post
    ... to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor... some in-depth grant writing tips at the Human Frontier Science Program's website. One way to add originality...get some critical insight into what you're doing wrong. All this helps to improve your application for ...

  7. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from...activated and inactivated transgene expression in neurons.  PubMed PMID: 2286602. PubMed Central PMCID: PMC3406316... Michelle Cronin...

  8. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ... affinity terminator may be desired for multi-cistronic constructs where high termination efficiency is...Bacterial Transcription Terminators: The RNA 3′-End Chronicles. Journal of molecular biology. 2011. PMID: 21439297...Ther. 2008. PMID: 18627247 Zufferey R, Donello JE, Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional...

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...possible because baculoviruses are slightly less prone to recombination than other viruses, like lentivirus.... (2002). Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...produce recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https:...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...genomic site can be edited based on its chromatin environment. After selecting the best pegRNA design with ...University of Zurich (Switzerland), where he developed a strong passion for computational biology. His expertise...s41587-023-01678-y Li, X., Chen, W., Martin, B. K., Calderon, D., Lee, C., Choi, J., Chardon, F. M., McDiarmid...

  11. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...Compensation beads are a convenient tool to obtain a strong positive signal for a given fluorophore, as they...won’t bind anything (negative population). It is strongly recommended that when you first run a new panel...compensation beads are convenient in that they provide a strong positive signal, even for rare cellular markers...

  12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...from two homologous strands of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA... for cancer therapy. Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan ... https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT...

  13. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ... 0 and 100 for each guide, with 100 being the strongest interaction between the guide and the target and...interactions (e.g., scores <20) are reduced to 0 and strong interactions (e.g., scores >80) are transformed...weak gRNAs (CFD < 20) bind almost not at all, and strong gRNAs (CFD > 80) bind almost as much as perfect...

  14. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...then, using multiple confinement strategies is strongly advised. The effects are multiplicative, and there...technology: because they necessarily alter the shared environment, they must never be used without popular support2...organisms and associated ecosystems.  Because the environment is shared by everyone, he is deeply concerned...

  16. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...cost-effective, timesaving for transformations. Cons: Prone to satellite colony formation on agar plates, less...less stable at high temperatures and in acidic environments.   Carbenicillin   Carbenicillin, colloquially...antibiotic than amp: it is more stable in high pH environments and is less susceptible to degradation in agar...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...adenovirus-based gene therapy clinical trial gone horribly wrong. It brought the whole field to a grinding stop and...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...Applications of CRISPR-Based Genome Editing in the Retina. Front Cell Dev Biol. 2018 May 14;6:53. Review. PubMed ...

  18. MXS Chaining

    Type
    Blog Post
    ...constructs were then combined to create a single polycistronic 15 kb insert. The final construct was then introduced...resulting HeLa cells show robust labeling, with strong expression and detection of each fluorophore at...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

  19. CRISPR Antimicrobials

    Type
    Blog Post
    ...antibiotic resistant cells could repopulate the environment after treatment. The phagemid systems used in...pathogenic or drug-resistant bacteria. It’s a bit ironic that CRISPR, the bacterial immune system, may someday...4. Yosef, Ido, Miriam Manor, Ruth Kiro, and Udi Qimron. “Temperate and lytic bacteriophages programmed...

  20. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work/life balance.... spouse won’t be at every school performance, chaperone every field trip or cheer at every soccer game...birth and breastfeed. Keeping this always at the forefront will go a long way toward alleviating the guilt...
Showing: 301 - 320 of 682 results