We narrowed to 313 results for: SPI

...100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down the cultures and use a miniprep kit to obtain...media from cells and pool with media from Day 4. Spin media at 1,250 rpm for 5 minutes to pellet any HEK...

...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

...transfer it into your container. Make sure that any spills produced during the protocol are cleaned up. Discard...

...Section 2: Fixing and permeabilizing cells Gently aspirate the media from the 24-well plate. Wash each well...

...CRISPR genome editing in dicotyledonous plants. CRISPi Kit Genome Engineering, CRISPR, Yeast Expression...

...isopropanol to precipitate the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column...

...Incubate the cells with the virus for 48–72 h. Gently aspirate the media from the cells. Add 1.5 mL of DMEM complete...

...transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host ...

... bit more than 200 mL (~220 mL) just in case we spill anything or have small errors in measurement. You...

...diseases in humans and other animals, most commonly respiratory infections, such as the common cold. The immune...

...desynchronization of neural activity, with single-spike temporal resolution. PLoS ONE, 2 (3), e299. https...

... of diseases such as inherited retinal disease, spinal muscular atrophy, hemophilia A and B, and Duchenne...

... NNGRR(N) Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) 5' TTTV AsCpf1 RR variant...