Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 541 results for: SHI

Showing: 321 - 340 of 541 results

  1. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered...effective for introducing non-sequence specific frameshift mutations through aberrant double strand break...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...Multiple uses: From multiplexing to multiscaling NIR-shifted spectrum is perfect for use in spectral multiplexing...autofluorescence, low scattering, and deep penetration of red-shifted light. The same NIR FPs and biosensors can be imaged...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...to understand. (1-2 sentences). For example: Distinguishing correctly folded and misfolded proteins is ...Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...develop a biosensor for oxytocin, Daisuke Ino and Hiroshi Hibino and colleagues devised a “Modular fluorescence...fluorescent biosensors for monitoring RTK activation by shift in cellular localization. Image adapted from Farahani...

  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked Immunosorbent Assay...Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA, ssDNA or RNA–protein...

  6. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...it. The other half of each day you can spend accomplishing small tasks (like... preparing plasmids to send... Send out as many emails as possible While accomplishing any one of these things won’t mark an end to...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...approximately 1 in 5000 males. DMD is caused primarily by frameshift mutations in dystrophin, a protein essential ...regions, so low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) ...

  8. A Control for All Seasons

    Type
    Blog Post
    ... western blot and look for the molecular weight shifts indicative of tau phosphorylation. By including... standard (P) to check for tau molecular weight shifts. A negative control is a sample or group that...

  9. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...experimental condition you could look for a slight size shift in the protein band before and after treatment.  ...0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender ...

  12. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...member who knows how the physical kits are made and shipped; Chiara, the tech transfer expert who understands...critical features based on my own experience: Leadership Few things are more frustrating than being on...

  13. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated...PMID:16550175. 6.Ando, Ryoko, Hideaki Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling...

  14. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...channels. In a typical assay a user will compare the shifting intensity of events. Since intensity positively...positively correlates with expression, the further the shift of a cell from the negative control, the higher ...

  15. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how...

  16. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure...PubMed Central PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements...

  17. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...Principal Investigator (PI), provide us with your shipping address for deposit materials, and agree to our...prepare your plasmids and a prepaid label with shipping materials to send your plasmids to us. Essentially...

  18. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over from the...bottles and the overall weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN...
Showing: 321 - 340 of 541 results