We narrowed to 882 results for: NSI

1. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   ...RNAi podcast segment RNA interference (RNAi) is extensively used in C. elegans research to study gene function...

2. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...

3. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

   ...function of immune cells that are not excitable but responsive to calcium. We’d love to expand our cancer and...

4. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   ...backing of SnapGene’s sequence viewer software and extensive feature library, our updated plasmid and sequence...

5. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   ...succeed in communicating. Even the most rigorous, insightful, and novel scientific research will be wasted...

6. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   ...alone. A few years ago when I started my career transition from bench science to software product management...

7. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   ...making your plates: X-gal is light and temperature sensitive and needs to be added to media after autoclaving...

8. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number...

9. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   ... activator-like effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they ...

10. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    ...and in vivo laboratory experiments ranging from transient gene expression in a particular cell type to CRISPR...

11. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    ...completely normal to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I'...

12. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    ...resources. There are three types of cognitive load: intrinsic cognitive load is the effort associated with a...

13. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    ...did not just automatically come to a solution. Consider the thoughts that went through your head as you...

14. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    ...Lerner RA, Wigler M (1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae...

15. RNA Extraction Without A Kit

    Type

    Blog Post

    ...samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of...

16. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    ...genes of interest. These transcription factors consist of the GAL4 or ZIF268 DNA binding domains fused...

17. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    ... new growth phase of the organization and the expansion of our impact, I’d like to take a moment and honor...

18. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene copy number based on the...

19. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    ...salary information as of December 1st. Salary inconsistencies across geographical regions When we originally...

20. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    ...Russell, P., & Guttman, M. (2014). Methods for comprehensive experimental identification of RNA-protein interactions...

21. dTAG - You're it!

    Type

    Blog Post

    ...or Jay Bradner ([email protected]). Considerations when getting the dTAG system up and running...

22. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    ...oxygen. Other useful backbone modifications to consider include amide linkages, unlocked nucleic acids...

23. Summer SciComm: Preprints

    Type

    Blog Post

    ...funding bodies and review committees encourage and consider preprints as demonstration of productivity. Part...

24. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    ...complementarity to the gRNA, they have a higher propensity to reform double helices and decrease Cas9 cutting...

25. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    ... to train our young scientists for futures both inside and outside of academic research (4). There are...

26. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    ...assembling the toolbox needed for anyone to begin considering genetic engineering projects in a very big way...

27. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    ... a number of different options. By carefully considering your ncRNA of interest, you should be able to...

28. Plasmids 101: Modular Cloning

    Type

    Blog Post

    ...Scripps Research, where he works on genetic code expansion.  References and Resources References Weber E...

29. CRISPR in the Clinic

    Type

    Blog Post

    ...Off-target effects are also important safety considerations for all CRISPR therapies, but especially concerning...

30. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    ...Thomas C. Evans, J., & Lohman, G. J. S. (2018). Comprehensive Profiling of Four Base Overhang Ligation Fidelity...

31. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    ...activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity to be ...

32. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    ... starting point for a new generation of ABEs), consists of 3 components:  a deaminase (TadA, originally...

33. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    ...functions of key regeneration-related genes in four inexpensive and commercially available planarians: Girardia...

34. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    ...cells are behaving normally can be prohibitively expensive for many labs. We’ve created and tested plasmids...

35. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    ...us at [email protected]. These deposits are considered large deposits and are exceptions to our standard...

36. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    ...full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science communication. The...

37. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...Arg-mutant proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering from ...

38. Hot Plasmids - October 2020

    Type

    Blog Post

    ...myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across these ...

39. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    ...of toehold switch-based RNA sensors within an extensive infrastructure of molecular and electronic companion...

40. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ...the target sequence (bases 19-23). Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver...

41. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    ...inverse Orientation), FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts...

42. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    ...it’s too inefficient and the reagents are too expensive to make it practical - but we have members looking...

43. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    ... from this data is that CRISPR technology is consistently showing rapid development and quick adoption...

44. Transferable Skills: Negotiation

    Type

    Blog Post

    ... on which lab jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists...

45. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    .... They will make mistakes and you will take responsibility (within reason!).  Try to buffer them from ...

46. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    ...deposition of the fertilized egg by its mother. Inside the pupal case, the larva will metamorphose into...

47. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    ...cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final...

48. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    ...own pros and cons (2). The NgAgo DNA guide is inexpensive and can be 5’-phosphorylated in house using a...

49. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    ...think plant biology is massively underfunded considering how important it is. I would really like to reach...

50. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    ...multi-spacer CRISPR array. Workflow and technical considerations Making insertions in bacterial genomes with...