We narrowed to 433 results for: Hal

1. What's New in CRISPR - November 2021

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   Blog Post

   ...cavity of Cas12f. The CasMINI system is less than half the size of the Cas9 or Cas12a based systems, making...

2. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

   ... M, Mueller SAL, Roseboom PH, Oler JA, Kalin NH, Hall RA, Smith Y (2019) Ultrastructural localization ...

3. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

4. dTAG - You're it!

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   Blog Post

   ...molecules by conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867...

5. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   Blog Post

   ...finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty is that some...

6. Human Germline Editing Using CRISPR

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   Blog Post

   ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

7. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...

8. RNA Extraction Without A Kit

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   Blog Post

   ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

9. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   Blog Post

   ... wild type  (Oveido et al., 2010). One of the challenges of studying bioelectricity is that it can be ...

10. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    ... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough...

11. Plasmids 101: Cre-lox

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    Blog Post

    ...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,...

12. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

    ...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...

13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

14. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post

    ...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

16. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

17. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ...students (1, 2), but educators face significant challenges in designing suitable projects. In the O'Connor...

18. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    Blog Post

    ...codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a ...

19. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ... make it difficult to detect proteins with short half-lives. Interested in using Split-BioID with your...

20. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...