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We narrowed to 741 results for: REV

Showing: 321 - 340 of 741 results

  1. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...cells compared to monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to... multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook & ...overgrowing the bacterial culture Another way to prevent plasmid multimerization is to avoid growing the...

  2. Water Bath Protocol

    Type
    Protocol
    ...water bath with distilled water, not tap water, to prevent salts from accumulating on the surfaces of the ...evaporates from the tub. Disinfectant may be added to prevent growth of bacteria or fungi. There are disinfectants...water bath cover on the top of the water bath to prevent evaporation and maintain the desired temperature...in the lab, you can leave the water in it from previous uses. However, you will still need to regularly...

  3. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...biased and unbiased approaches. As suggested in a review by Tycko et al., 2016, unbiased sequencing and ...High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature...specificities of CRISPR-Cas9 nucleases." Nature Reviews Genetics17.5 (2016): 300-312. PubMed PMID: 27087594...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...ITR: ITR Forward Primer: 5’-CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ...Forward ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Reverse ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Nuclease-free...tube, gently uncap the tubes. Add 5 µL of the previously aliquoted 1X dilution buffer from step 9 to the...titering using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ... affects the solubility of the RNA. Pro-Tip To prevent overdrying, watch the pellet and carefully remove...for RNA. Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ... for RNA.Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...of inosine from DNA is likely infrequent, thus preventing the induction of base excision repair. In terms...improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve 98-99%...

  8. Deck the Lab 2022!

    Type
    Blog Post
    ...DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have decorated their labs with...on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram! Happy...

  9. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...microbiology lab every year for over 20 years. Previously, the lab used three different species for the...continues until all quadrants are used, hopefully revealing single, isolated colonies in the last quadrant...

  10. Weighing Reagents Protocol

    Type
    Protocol
    ...might need to weigh it out in the fume hood to prevent exposure. Read the reagent’s material safety data...used to contain the material you’re weighing and prevent cross-contamination with other substances. They...spatula is clean, and sterile (if necessary), to prevent any potential contamination. Scoop out the material...

  11. Let There Be LITE Plasmids

    Type
    Blog Post
    ... expression of specific genes in the brain in reversible fashion. And that’s exactly what the LITEs now...response can be graded with light dose and is fully reversible after light retraction. Finally, because light...

  14. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ... stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins are overexpressed...protein expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology...

  15. Science as Art

    Type
    Blog Post
    ...x-ray machine not on teeth, but on blossoms, thus revealing the startling inner beauty of familiar flowers...such as those collected by Darwin on his voyage. Previous destructive techniques were incompatible with ...

  17. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian ...multiplexed gRNAs in human pluripotent stem cells. Previously, CRISPRi or CRISPRa depended on continued expression...

  18. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...assembly from cryo-electron microscopy, the team reveal the mechanism behind its activity including an ...motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform pooled...

  19. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding... resistance. Much of plant genetic engineering revolves around Agrobacterium tumifaciens. Agrobacterium...

  20. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...transcriptional activation/repression. dCas9 has also previously been adapted to fluorescently label a sequence...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...
Showing: 321 - 340 of 741 results