...background staining. This can include using: different blocking reagents; titration of antibody concentration ...

...gene body can repress transcription by physically blocking the elongation complex from binding the DNA or...

...mutations to alter the absorption spectrum or adding trafficking signals to localize opsins to the cell membrane...activity 589 eNpHR 3.0 NpHR with addition of trafficking signal from Kir2.1 and ER export signal provide...sodomense 566 eArch 3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated ... Arch. 566 eArchT 3.0 ArchT with addition of trafficking signal from Kir2.1 566 Leptosphaeria rhodopsins...Leptosphaeria maculans 540 eMac 3.0 Mac with addition of trafficking signal from Kir2.1 540 Plan Your Optogenetics... to be sequestered Lumitoxin Peptide toxin for blocking K + (Kv) channels fused to LOV2 photoswitch. Illumination...

...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living...inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition; inhibiting Cas9...sites is sufficient to repress transcription by blocking initiation. More robust transcriptional repression...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015)...

...vectors are based on Ad5. Ad5-based vectors use the Coxsackie-Adenovirus Receptor (CAR) to enter cells. Recombinant...contain GFP Additional Vocabulary Term Definition Coxsackie-Adenovirus Receptor (CAR) Receptor used by Ad5...

... number of E. coli strains below and recommend checking out these two Addgene blog posts relating to common...and their recommended concentrations. We suggest checking your plasmid's datasheet or the plasmid map to...

... Negative Screen Negative screens are a little trickier than positive screens. In a negative screen, the...

... the observed efficiency of CRISPR knockout or knockin. Select gene and genetic element to be manipulated...

...antipsychotic drugs: is M1 agonism a pre-requisite for mimicking clozapine’s actions? Psychopharmacology. 178:451...

...unused surface-binding sites in the wells with a blocking protein such as BSA, followed by several wash ...