We narrowed to 334 results for: cki;
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TypeBlog Post...Relationship building is key in science policy. Luckily, there are many mechanisms in place to ensure your...
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When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... a method relies on accurate ligation of nicks lacking gaps or mismatched base pairs (as in the case for... -
Transferable Skills Guide: Teamwork
TypeBlog Post...yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...like to investigate in your next CRISPR screen. Luckily, the Green Listed software can help you do just... -
Antibodies 101: Flow Cytometry
TypeBlog Post...43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry: An Overview. Current... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...(not expressing the opsin) are also available. Picking your opsin You must consider whether you wish ... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...ethanol, or endotoxins. The most common method for checking both quantity and quality of your plasmid prep... -
Antibody Validation for Flow Cytometry
TypeBlog Post...background staining. This can include using: different blocking reagents; titration of antibody concentration ... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...Matoba, S., Zhang, Y., & Zhang, F. (2013). Double nicking by RNA-Guided CRISPR CAS9 for enhanced genome editing... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...gene body can repress transcription by physically blocking the elongation complex from binding the DNA or... -
Guide to Using Pooled Libraries
TypeGuide... Negative Screen Negative screens are a little trickier than positive screens. In a negative screen, the... -
Chemogenetics Guide
TypeGuide...antipsychotic drugs: is M1 agonism a pre-requisite for mimicking clozapine’s actions? Psychopharmacology. 178:451... -
Antibody Guide
TypeGuide...unused surface-binding sites in the wells with a blocking protein such as BSA, followed by several wash ...