...with people, nurturing networks, as much as with technical knowledge. I leverage a breadth of knowledge to...I am constantly teaching – often explaining a technical piece of research to others outside that specialty...punctuation can be wildly funny as well as financially critical. Those who work on the publishing side of science...

...system by using a stronger promoter and different biochemical secondary energy sources to increase protein ...DNA of interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free...sgRNA pool can also target core operons in other pneumococcal strains. The BARBEKO sgRNA library uses CRISPR...

... lab at ETH Zürich. “Instead of a graphical abstract, it’s a musical abstract,” says Di Ventura, a professor...University of Freiburg. This paper is about a new tool called BLADE (blue light-inducible AraC dimers in E. coli...

...the less acknowledged perks of scientific and technical training is that these educational paths prepare...Foundation study showed that 57% of PhDs in US Biomedical workforce will NOT go into “traditional” academic...but there is more flexibility with the academic calendar Academic researchers must be extreme self-starters...

...particle to infect a cell, it has to come into physical contact with that cell. Since there is a limited... by an anterograde AAV was shown to drop off drastically with lower titers. As you can see in figure 2...AAV-mediated gene expression in a target cell. Practical considerations If you have high quality AAV, you...

...the host university does not hire or pay them (typically). A visiting scientist is distinct from equipment...the scientist to the new institute where their typical workdays will be carried out until the end of the...one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist positions...

... and 15/17 morphologically aligned. Documentation of the network that initiates biological reprogramming...10.1016/j.cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and transcriptional activation...

...30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy by: Susanna Stroik...many of these applications have lagged. Fixation, chemical staining, and light exposure can negatively affect...Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy...

...virtual conferences may have apps or interactive calendars that you can use to help plan your conference ...one session to another,” Bachle says. “Now, the physical location didn’t matter. I didn’t have to consider...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...

...for actually making it happen would increase dramatically. I’m not sure what the best approach to doing...at The Scripps Research Institute in La Jolla, California. She joined Elsevier after her postdoctoral studies...primary research and review journals across life and physical science, and editorial contribution to the strategic...

... to a method of physically joining multiple fragments of DNA to create a synthetically designed DNA sequence...one single-stranded oligo, instead of the more typical two oligos, is more cost effective and eliminates...

...it’s now easy to monitor protein expression, localization, and protein-protein interactions. Beyond these...known to bind to the HMO of interest. Fatima specifically proposes using this sensor to detect HMOs found...Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional Resources on the Addgene ...

...Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary...the papers with plasmids deposited at Addgene – typically about four times as many as papers without plasmids... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...

... reader accessing the information? This can be physical or digital. For instance, you would present information...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

... light-mediated? The researchers split the proteolytically active light chain of BoNT/B into two fragments...rely heavily on fluorescent protein genes. These optical reporters have limited deep-tissue performance,...the field. Arlene Sharpe’s group at the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9...

...as help our students succeed whether that be academically or on the employment front. Paratore: I work...& Morning. Karen was a founding member of Mass Medical Angels, a venture mentor at MIT and served on the... the Greater Boston Food Bank and participates locally in Read To A Child's "Lunchtime Reading Program...

...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...different constant domain gene. This process is called isotype switching and results in the B cell expressing...

...early sets of SARS-CoV-2 materials, it will not scale well to the several thousand plasmids from Ginkgo...the samples, the rest will be put into a status we call “Archived.” This status allows us to store plasmids...and we’re glad to be able to do so during this critical time. Visit our COVID-19 Plasmids and Resources...

..., a powerful open-source search engine that can scale to search millions of web resources and handle millions..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms...PI labs and institutions. There are many more technical details that make our new search engine unique...

...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...Have you or others been able to successfully systemically transduce and then retransduce a model organism...as multiple sclerosis?  A: Basic science and preclinical researchers have used the flexibility of AAV ...

...study it. The process is fairly straightforward. Basically, you place your gene of interest in a plasmid ...genetic crosses with balancer chromosomes to make genetically stable fly lines. After a few weeks of waiting... aware of at least some of the possibilities. Basically, if you can devise an experimental assay that ...

...North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the Allen ...educational materials, and provides viral vector technical service (details about virus production protocols...word out about this new resource and providing fantastically useful educational content on viral vectors....

...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification...Well, even though Dam methylation sites are not specifically associated with any restriction modification... These specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient...

...reprograming influence tumors development? Being pathological active regions, microenvironments shape a tumor...interested in science journalism and writing about biomedical issues. Follow her on Twitter @BrbaraPinho6. ...expression predicts early tumor recurrence and poor clinical outcome in human breast cancers." The American...

... rate would need to be much, much lower before clinical applications could be considered. It is important... efficiency and applicability. Given moral and ethical concerns surrounding such research, it is crucial... experiments are needed before we can consider clinical applications for germline editing. Although this...

...developed cloning vectors to harness it. These vectors, called pKIL18 and pKIL19, contained the ccdB gene in frame...ccdB and therefore would die. This procedure dramatically reduces the number of clones that do not contain...and ccdB Survival strains have been developed specifically with ccdB-containing vectors in mind, any plasmid...

... year, narrow by article type (review, patent, clinical study, research article etc). There are also numerous...conclusions. In any case, the reader should give a critical eye to all results in the relation to how they... biochemistry instructor in the Department of Biological Sciences at Louisiana State University. She is...

...bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud...individually. Enter the BIOROBOT! This robot is called the Qiagen BIOROBOT Universal Platform and it suits... for a mini prep, except it does it on a larger scale. Using the BIOROBOT Universal Platform is ideal ...

...., 2021). Another version of the spike protein, called HexoPro, was developed by Jason McLellan’s lab....genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation), from Addgene to better understand...bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection. ...

...Addgene’s Lab Manager, was taking classes at her local community college when she took her first job at... and hoping to pursue. The choice to not go to medical school was devastating. At the same time, she has...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...

... to be re-engineered for each gene, making wide-scale gene activation seem next to impossible. The development...methods greatly improved CRISPRa, expanding its practicality and popularity in research (Tanenbaum et al....JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional activation by an engineered CRISPR-Cas9...

...unintelligible to a lay audience. My lay paragraph typically includes a couple of sentences of introduction... rejected on some occasions. Strategize, choose calls that suit your career stage / research area, and...peers and collaborators can also help you get some critical insight into what you're doing wrong. All this...

...remarkable progress on establishing a system for the chemical replication of RNA in the absence of evolved enzymatic... about using CRISPR/cas to address the growing clinical problem of antibiotic resistant bacteria. See ... and Sun Yat-sen University presented two new technical developments. Using the Tet-on system, his lab...

...edits or knock-ins. Editing efficiency decreases drastically once your cut site is more than 10 bp away from... mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created a... a selection phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit...

...sequencing primers for quality control. At such a scale, automation is crucial, both for speeding things...to the same principles listed here. It employs biological best practices. For example, it ranks guides ...published on how to do it best. Scientists are typically advised to mutate the PAM to which the guide binds...

...but close together in terms of where they are physically located. DNA is then sheared into pieces small...on-target enrichment. 2. Determining the spatial and hierarchical organization of chromatin interaction networks...CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction networks. Think of these...

...bicistronic vectors take advantage of an element called an Internal Ribosome Entry Site (IRES) to allow...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...journal.pone.0018556. Epub 2011 Apr 29.) PubMed. 5. Scalable signaling mediated by T cell antigen receptor-...

...not the case for knock-ins. Knock-in mutations typically require an exact DNA sequence to be incorporated...know that HR is often mutated in cancer cells, specifically in breast and ovarian lines commonly used in...common editing pathway for DNA repair. To tilt the scales in your favor for an HDR edit, you can try one ...

...-55 °C for the homologous region (calculated using Oligo Calculator). Insertions - To insert short sequences...parental DNA (37 °C for 15 mins). This enzyme specifically cuts methylated DNA, so it will selectively ...

...Liang et al. 2017).  PEG-mediated methods, while technically less difficult to perform, often show low efficiency...ribonucleoprotein electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215...Cell-Type-Specific Gene Editing." Journal of the American Chemical Society 140.21 (2018): 6596-6603. PubMed PMID:...