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We narrowed to 394 results for: AGA

Showing: 341 - 360 of 394 results

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Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent...
Site Directed Mutagenesis by PCR

Type

Blog Post

...fragments should be separated on a high percentage agarose gel (~3%). Note that primer duplication will evade...
Evolution of Lab Techniques

Type

Blog Post

...restriction enzymes and then running the products on an agarose gel allows a researcher to properly orient specific...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

...Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

...experimental period, as this mark was faithfully propagated in culture and in vivo. In certain cases, epigenetic...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Type

Blog Post

...directly by creating well characterised antibodies against all human extracellular proteins with community-based...
Antibodies 101: The Basics of Western Blotting

Type

Blog Post

...blocked, it is stained with a primary antibody against your protein of interest. But which antibody should...
Finding Your Perfect Job After University

Type

Blog Post

...Working in industry: virology On completing my MSc, I again had to look at what type of work I would like to...
When Fidelity Matters: A frank discussion about ligase fidelity

Type

Blog Post

...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches...
Transferable Skills Guide: Teamwork

Type

Blog Post

...matter whether that other person really is acting against the team’s interests or not; once that seed of ...
Quick Guide to All Things Lentivirus

Type

Blog Post

...multiplicity of infection (MOI=ratio of virus to cells). Again, my tip here is to test several protocols as the...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

...temperature allows the repressor to re-nature and again repress expression of exo, beta and gam. A major...
Sequencing Options for CRISPR Genotyping

Type

Blog Post

...equitable society. Read more at https://magazine.scienceforthepeople.org/. References 1. Fu, Yanfang, et...
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

Type

Blog Post

...j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

...briefly go through each section of this article again. In the beginning, we talked about the relationship...
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Type

Blog Post

...complexes - from the cell-lysate using antibodies against the target protein. The captured protein complexes...
Custom CRISPR Screens & the Green Listed Software

Type

Blog Post

...Another interesting approach is to design screens against known druggable targets, preferentially those for...
A Primer on Optogenetics: Introduction and Opsin Delivery

Type

Blog Post

...for sufficient opsin to reach the axon terminal. Again, this must be empirically determined in your own...
Chemogenetics Guide

Type

Guide

...olanzapine has also been shown to be an agonist against hM4Di, and is especially attractive for use in ...eaav5282. PMID: 30872534 (Link opens in a new window) Nagai Y, Miyakawa N, Takuwa H, Hori Y, Oyama K, Ji B, ...
A Needle in a Base-Stack: Cas9 Structural Biology

Type

Blog Post

...precise location. The end of the heteroduplex rests against the RuvC nuclease domain, which stabilizes the ...

Showing: 341 - 360 of 394 results

Results per page:

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Site Directed Mutagenesis by PCR

Evolution of Lab Techniques

Viral Vectors 101: AAV Serotypes and Tissue Tropism

CRISPR 101: Epigenetics and Editing the Epigenome

Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Antibodies 101: The Basics of Western Blotting

Finding Your Perfect Job After University

When Fidelity Matters: A frank discussion about ligase fidelity

Transferable Skills Guide: Teamwork

Quick Guide to All Things Lentivirus

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Sequencing Options for CRISPR Genotyping

A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

Antibodies 101: Designing Your First Flow Panel

Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Custom CRISPR Screens & the Green Listed Software

A Primer on Optogenetics: Introduction and Opsin Delivery

Chemogenetics Guide

A Needle in a Base-Stack: Cas9 Structural Biology

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