We narrowed to 398 results for: AGA

1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   ...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent...

2. Five Popular Model Organisms, Part 2

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   Blog Post

   ... to be replicated. Because E. coli are easily propagated and have a generation time of around 20 minutes...

3. Antibodies 101: Normalization and Loading Controls for Western Blots

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   Blog Post

   ...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...

4. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   ...template that corrects the blocking mutation and again identify the right clone from a few hundred. From...

5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   Blog Post

   ...in the same orientation as those in the plasmid. Again, if they are in the opposite direction, both strands...

6. Site Directed Mutagenesis by PCR

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   ...fragments should be separated on a high percentage agarose gel (~3%). Note that primer duplication will evade...

7. Evolution of Lab Techniques

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   Blog Post

   ...restriction enzymes and then running the products on an agarose gel allows a researcher to properly orient specific...

8. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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   Blog Post

   ...directly by creating well characterised antibodies against all human extracellular proteins with community-based...

9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   ...Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016...

10. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...experimental period, as this mark was faithfully propagated in culture and in vivo. In certain cases, epigenetic...

11. Antibodies 101: The Basics of Western Blotting

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    Blog Post

    ...blocked, it is stained with a primary antibody against your protein of interest. But which antibody should...

12. Hot Plasmids: Spring 2025

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    Blog Post

    ... set of highly-specific recombinant antibodies against three of the six human glypican proteins (GPC1,...

13. Finding Your Perfect Job After University

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    Blog Post

    ...Working in industry: virology On completing my MSc, I again had to look at what type of work I would like to...

14. Chemogenetics Guide

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    Guide

    ...olanzapine has also been shown to be an agonist against hM4Di, and is especially attractive for use in ...eaav5282. PMID: 30872534 (Link opens in a new window) Nagai Y, Miyakawa N, Takuwa H, Hori Y, Oyama K, Ji B, ...

15. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches...

16. Transferable Skills Guide: Teamwork

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    Blog Post

    ...matter whether that other person really is acting against the team’s interests or not; once that seed of ...

17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ...j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH...

18. Quick Guide to All Things Lentivirus

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    Blog Post

    ...multiplicity of infection (MOI=ratio of virus to cells). Again, my tip here is to test several protocols as the...

19. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    Blog Post

    ...temperature allows the repressor to re-nature and again repress expression of exo, beta and gam.  A major...

20. Sequencing Options for CRISPR Genotyping

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    Blog Post

    ...equitable society. Read more at https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et...