We narrowed to 444 results for: ENA

1. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   ...you can start by asking ‘What is the biological phenomena I want to observe?’ This information, along with...

2. High Complexity Golden Gate Assembly with NEB’s DAD

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   Blog Post

   ... K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of unprecedented...

3. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ... and treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving patients...

4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   Blog Post

   ...PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

5. Academic vs. Industry Postdocs

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   Blog Post

   ...are busy with their pipeline projects. In this scenario, finding someone with whom you can have an in-...

6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...form complex, multi-insert modular assemblies that enable biosynthetic pathway engineering and optimization...

7. Plasmids 101: 5 factors to help you choose the right cloning method

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   Blog Post

   ...number of inserts ...as some methods are more amenable to combining multiple DNA fragments than others...

8. Plasmids 101: Protein Expression

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   Blog Post

   ...level recombinant protein expression. These systems enable high expression of very complex, glycosylated proteins...

9. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ...decade. CRISPR screens are easily customizable and enable the targeting of hundreds to tens of thousands ...

10. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

    ...import, and neuronal trafficking. Although FPs have enabled discovery of many cellular mechanisms, there are...

11. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post

    ...https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia O (2011) Vector Characterization Methods for Quality...

12. Transferable Skills: Negotiation

    Type

    Blog Post

    ...about the individuals, each example provides a scenario where both people get something valuable. Importantly...

13. Plasmids 101: Degron Tags

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    Blog Post

    ... other unique features. If your protein is not amenable to being tagged, e.g. tagging some proteins can...

14. Of Myc and Men

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    Blog Post

    ...1997) with the goal of making the antibody more amenable to protein engineering. As a result, you can now...

15. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    ... to see what new editing possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016)...

16. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...information about how the vector was assembled, and enables requesting labs to utilize and modify the plasmid...

17. Genome-wide Screening Using CRISPR

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    Blog Post

    ...compared to a non-treated control group. In this scenario, gRNAs that are “enriched” correspond to genes...

18. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...with techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture...

19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post

    ...biology and genetics because the underlying advance enabling RNA-guided gene drives is Cas9 genome editing....

20. Plasmids 101: Multicistronic Vectors

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    Blog Post

    ...interest at high levels. Unlike vectors that express screenable or selectable markers from a unique promoter,...