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We narrowed to 658 results for: EPO;

Showing: 341 - 360 of 658 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...Calcium Ratiometric calcium biosensors from nested reporters of green- and orange-emitting proteins Ratiometric...imaging (Perceval) A genetically encoded fluorescent reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2)...

  5. Bacterial Expression Systems

    Type
    Collection
    ...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent ...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...it. Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins...

  6. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...system, Ye’s lab could edit a tdTomato fluorescent reporter gene in mice using Minicircle DNA vectors encoding...seen in the figure below. Next, by using a dual reporter fluorescence assay, Hemphill et al. demonstrated...

  7. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    .... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...Addgene.org CRISPR guide CRISPR protocols by Addgene depositors gRNA design software CRISPR plasmid collections...

  8. Promega Plasmid Collection

    Type
    Collection
    ... and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light produced...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing... Depositor Collections Promega Plasmid Collection Promega... workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...collection. For example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase"... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from Promega...

  9. Rett Syndrome

    Type
    Collection
    ...Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository, including...in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in a new window...largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository Tissue...MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) PMID: 35148843...window) Jackson Labs - mouse line developer and repository, and CRO for centralized behavior scoring (Link... purposeful hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens...contributing your plasmids! To get started with your deposit, please click here ....

  10. Plasmid Collections

    Type
    Collection
    ...shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids from a depositor organized by a common...Collections Addgene Plasmid Collections Addgene's repository contains 162,657 plasmids . We've gathered our...our curated plasmid resources, kits, and depositor collections on one page to help you find what you're...looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources group...common use. Click here to view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein...community that has established a common place for deposited plasmids. ASAP Discovery Consortium ARF GTPase...Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific disease research areas. ...

  11. Synthetic Biology - Overview

    Type
    Collection
    ...the community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse ...available at Addgene. Browse featured collections or depositing labs. Plasmid...Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks JBEI - Joint Bio-Energy...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for... Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing...pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase...destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...

  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...page for more information Genome Modifications, Reporter Assays, mRNA Regulation, and More Find empty backbones...backbones for other uses such as genome modification, reporter assays, shRNA expression, transgenics, and more...TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection of plasmid backbones...genes can be inserted into this firefly luciferase reporter to test for their effects on protein production...

  14. COVID-19 Resources

    Type
    Collection
    ...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ...previously we will reach out to the lab to ask them to deposit the plasmid with us. Return to top Pooled Libraries...

  15. Microbiology Resources

    Type
    Collection
    ... aren't in the repository? Help us widen our resources for the community by depositing your useful tools...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...bacteria, and more. Synthetic Biology : Browse our deposited tools and resources, or read our guide on assembly... reference page for CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast...Genome Engineering blog post to see how Addgene depositors are making this possible in a range of organisms...

  16. CRISPR Plasmids - Tagging

    Type
    Collection
    ...The Eric Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system to add a tag...homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas tagging system were... Tagging System Recently, Yannick Doyon's lab deposited a plasmid which introduces an N- or C- terminal...PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based knock-in...microhomologous sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...

  17. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... Depositor Collections Bikard Lab - CRISPR Repression Collection...David Bikard Lab at the Institute Pasteur has deposited a collection of CRISPR arrays to modulate the ...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...

  18. Distribution to Industry

    Type
    Collection
    ...Addgene's deposit agreements allow Addgene to distribute plasmids on behalf of the depositing institutions...proteins that are structurally characterized (PDB-deposited), disulfide-free, mesophilic, and designed to ...legal agreement between the recipient and the depositing institution(s). It governs the transfer of biological...entity is qualified to receive materials from our repository. All purchases are subject to Addgene's Terms...

  19. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core... Depositor Collections...window) of the Baylor College of Medicine have deposited a series of lentiviral and adenoviral transfer...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...
Showing: 341 - 360 of 658 results