We narrowed to 469 results for: LARGE

1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   Blog Post

   ...double-stranded DNA transgene is incorporated into a large complex of both viral and cellular proteins and ...enzymes that are proteolytically processed from the larger protein: PR (protease), RT (reverse transcriptase...

2. Antibodies 101: Beyond Surface Labeling

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   ...cells compared to dead cells. As a result, the largely negative live cells can still be easily gated on...

3. An Integrin Antibody Toolkit from IPI

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   ...Both subunits have short intracellular ends and large ectodomains containing ligand-binding heads and ...

4. Multiple Plasmids at a Low Price: Kits from Addgene

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   ...changes in enzyme function can lead to surprisingly large fitness effects during adaptive evolution of antibiotic...

5. Using Phosphoserine to Study Protein Phosphorylation

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   Blog Post

   ...Tomida, J., et al. "Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing...

6. Pipetting Protocol

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   Protocol

   ... liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid, tilt...

7. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

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   Protocol

   ...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ...

8. 28 Hot Plasmid Technologies from 2015

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   Blog Post

   ...modulate biological processes, but it did not show large changes in binding affinity with light stimulation...advantageous compared to other viral systems due to its large cargo capacity, minimal cytotoxicity, and broad ... CRISPR-Display uses the dCas9 enzyme to deploy large RNA cargos to specific DNA loci. In CRISPR-Display...

9. CRISPR 101: Epigenetics and Editing the Epigenome

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   ...) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging into...genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9...

10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...on many different levels - it’s hard to process large amounts of information and, from a SciComm perspective...results. As biological researchers deal with larger and larger datasets and use computer based analysis techniques...

11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post

    ...models remain more difficult to make. For example, large insertions, like a fluorescent protein knock-in,...

12. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ... without causing issues associated with cloning large fragments. After fragmentation, repair sheared ends...

13. Deep Dive: Statistical Tests (Comparisons)

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    ... to reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this...arranging all calculated p values from smallest to largest and ranking them (1 being the smallest value). ...calculates the cut off for significance by finding the largest p value where its rank divided by the total number...

14. Plasmids 101: Repressible Promoters

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    Blog Post

    ...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...

15. 22 Hot Plasmid Technologies from 2014

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    Blog Post

    ...mechanism for sgRNA delivery and providing a means for large scale functional screens. For more information on... kinases Receptor tyrosine kinases (RTKs) are a large class of cell-surface receptors which play a critical...

16. Transfection for Recombinant Antibodies

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    Protocol

    ...replication use a HEK293 line stably expressing the SV40 large T antigen. For antibody-expressing plasmids containing...

17. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ...their environment. These types of interactions to a large extent involve receptors expressed on the cell surface...

18. Important Considerations When Using AAVs

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    Blog Post

    ...preferential tropism for different brain regions is largely due the ability of different serotypes to infect...is fine and a researcher should aim to inject a larger amount of vector using a serotype with strong CNS... of adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal...

19. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    Blog Post

    ...The best applications for dsDNA inserts include: large insertions or deletions, including selectable DNA...

20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

    ...Getting a vector into a particular cell type depends largely on the viral capsid, but specificity doesn’t end...