We narrowed to 628 results for: PROC
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TypeBlog Post...not always guaranteed. To learn more about this process check out our Mouse Modeling blog posts: Part 1...have germline competent ESC lines. Second, the process is much quicker. It takes 3 months to generate ...
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Finding Your Perfect Job After University
TypeBlog Post... controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly...the technology is so advanced that most of the procedures in the lab are done with robots. Young scientists... -
Quick Guide to All Things Lentivirus
TypeBlog Post...your virus. Virus purification can be a tricky process. You have to centrifuge the culture supernatant...that favours virus precipitation. My tip for this process is to test several protocols that have been described... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post... CRISPR/Cas9 gene editing process. See below for details. The entire process from start to finish took... -
9 tips for a successful postdoctoral experience
TypeBlog Post...career and you'll become a better scientist in the process. 9. Avoid burnout The suggestions in this blog ...a diet rich in vegetables, foods with minimal processing, and low in empty calories will make you feel... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...optogenetics to study physiological and behavioral processes (Abdallah et al., 2018; Deubner et al., 2019)....induces reanimation from general anesthesia." Proceedings of the National Academy of Sciences 113.45 (2016... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...incorporation of that sequence into the zygote’s genome, a process that’s highly unpredictable. Transposons, however...injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA is injected. Sleeping... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...medical practice, and understanding the scientific process can help us understand how new therapies can be...experience. Previous Addgene blogs have discussed the process that goes into choosing a good scientific mentor... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...most important steps in the CRISPR experimental process is validating edits. Regardless of which CRISPR...not an edit in the target gene. The validation process, also known as CRISPR genotyping, is critical to... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post... eliminate cancer cells. 2. Equilibrium - In a process analogous to the development of drug resistance...system, tumors “learn” how to escape it. In this process, cancer cells develop and thrive within what is... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...might feel disconnected from the entire scientific process. I've spent a lot of time debating how to fix ... science in May, I marched to advocate for the process we use to learn about the world. In a way, I marched... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...indicating that there is more to learn about this process! Figure 3: Crystal structure of S...made! Conclusion Finally, we can summarize the process from start to finish: Figure 9: Cartoon... -
Deep Dive: qPCR
TypeBlog Post...Getting your RQ value isn’t the end of your analysis process. You’ll still need to do statistical analysis to...Gene Expression Analysis During iPS Reprogramming Process. Sci Rep 8, 8716 (2018). https://doi.org/10.1038... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs, particular for those...recombination vectors. The efficient and flexible cloning process provides improvements to classical cloning methods... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog Post...Sorting” is there to help guide you through the process. If you’re confident in your application and protocols... -
Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!
TypeBlog Post...Photocleaveable PhoCl protein for controlling molecular processes with light. Listen to Our Segement on PhoCl Segment... -
Enabling high school research at the Journal of Emerging Investigators
TypeBlog Post...networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target audience and their... -
New Videos: Addgene Lab Tips
TypeBlog Post... time, our lab has gotten a lot experience in processes including automated vial filling, accurate tube-labeling... -
Twenty Years of Sharing Science!
TypeBlog Post...support to our efficient and easy tech transfer process to our website and educational resources. We've... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post... Service Learn more about our Viral Production Process Find other Viral Vectors ...