...The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that ...commonly available tags and their antibodies along with some pros and cons. FLAG FLAG is a synthetically derived...immunoprecipitation, western blot, and immunofluorescence with some success for protein purification as well. This tag...a protein may disrupt the biological function of some proteins. Protein folding issues, steric hindrances...

... most chromosomes are represented by two to three copies, but several have four. No Y chromosome is present...gland is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t ...advantage to use the cell line and we will highlight some of those applications here.   Virus production HEKs...

...with the condition to reveal some “outstanding differences.” They now have some theories on the underlying...on rare diseases, offering them the chance to win some of the latest tools and technologies in life sciences...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012...

...unwavering devotion to seeing your ideas become something tangible.  When you think of entrepreneurship ...multibillion dollar tech companies using only laptops and some server space. But what if you want to be an entrepreneur...heavy price tag, especially if you're working on something other than software.  If you are a graduate student...experiences founding and running miLab to describe some key lessons I learned while becoming a health science...

...genetic tools are available. While he recognizes that some beginners may have a tendency to shy away from ratiometric...he recommends they give it a shot. “Beginners sometimes think it’s complicated, but actually it is very...of success – whether we have really developed something useful [enough] that other people start using ...Check out our Hot Plasmid page, where we highlight some of the popular & newly available plasmids at Addgene...

... mentor, developed some strategies for finding mentors and, perhaps, asked someone to support your career...identify areas that need work or growth. Here are some ideas for making your meetings productive: Discuss... posse holds me accountable for attending and sometimes it is only the knowledge that they will be watching...

...students! After a course coordination meeting with some other professors, I went home thinking about how...When I got home, I saw my daughters playing with some cards and I had a quick flashback to time spent ... most of the concepts required by the course and some were so enthusiastic that they took the cards and...gamification” will lead to new cures for disease someday! Accessing and modifying BUILD YOUR PLASMID You...

... news and there’s bad news. The bad news is that some of tubes’ o-rings are, in fact, permeable to CO2...information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples on dry... dry ice - it may prevent you from seeing some unexpected results. Order Ready to Use Viral Preps from... can affect the pH of the sample, which may have some effect on the contents of the sample. For example...

...take responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately...Specify desired results. Are you delegating to someone with sufficient skills and experience? Do you think... think you are giving this responsibility to someone who can do the job. It is good to delegate so the...and resources needed for the assignment? Setting someone up for failure by delegating is a bad idea. How...

...Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for some quick advice and resources for hiring research staff... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement...The Howard Hughes Medical Institute (HHMI) offers some great resources for Early Career Scientists, including...

...manufacturer, lot number, and expiration date somewhere you can easily check it whenever you use the antibody...freeze-thaw cycles, as they’ll degrade your antibody.  At some point in your long and illustrious lab career, you... with other chemicals and biological structures, some of which you may need in your experiment.  If you... to buffers, can bind to amine groups present in some conjugates, blocking the conjugation reaction. Many...

...set up these systems. Components of a biosensor Some of the biosensors used in research are very sophisticated...another factor, the receptor. The receptor could be something like an antibody, enzyme, DNA, etc. The receptors...in multiple species, and in cell culture models. Some of them have even been expanded for multiplexed ...and safety tips. Still worried? We make virus with some of our most popular biosensors already packaged ...

... all the Cas enzymes you know and love, and even some you’ve likely never heard of. Each entry displays...adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays these requirements ... orientation relative to the gRNA (5’ or 3’). In some cases, there is no requirement, which is also documented...CasPEDIA.   Helpful resources and information For some researchers, the previous information may be all...

... of these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we ... rare variants that haven’t been characterized? Some have developed exciting computational algorithms...acid change based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often...genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, Wu X, et al. Cancer...

...myself would take less time than the seemingly burdensome task of explaining the steps to a lab assistant...lab scientist, I worked with a diverse team where some members thrived on independence, while others required...dynamics. I experienced this firsthand when it took me some time to adapt to the communication style of a new...do this while changing industries and positions. Some people discover they truly enjoy management and ...

...that didn’t get answered live - but he kindly took some time to address all your unanswered questions via...be oversized even before considering the need for some manner of polyA. In this scenario, you may be best...adopting ddPCR, which does not a standard curve and somewhat more reliable quantitation. Differences in sample...VAST) experiments using PHP.eB in the brain give some insight into codelivery efficiency at different ...

...expensive because they require particular enzymes, some of which are proprietary. These methods also rely...bacteria do the work If you’ve conducted SDM using some of these methods, chances are you’d like to find... containing the mutations, the primers also have some homology to each other; ~17 bases is optimal. After...a whopping 144 kb from a bacterial artificial chromosome in just one reaction. Using other methods, such...

...month formal program. After the formal program ends, some 50% of these groups continue to meet regularly, ...development plans. However, they find it awkward to ask someone for the time commitment of a formal mentoring relationship...stage, they have a lot to learn from each other. Some have taken my advice and I recently heard from peer...organizations as it brings a diversity of perspective. Sometimes it is helpful to find peers that share a similar...

...so-called “small molecule degraders” recruit the proteasome to destroy targeted proteins. We previously developed...polyubiquitination of the target protein and proteasomal degradation. Remarkably, small molecule degraders...dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We are particularly...between the FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon, leading...

...ones to try?  Read on and we will provide you with some advice on the antibody selection process. Antibodies... is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated...used in a publication. Talk with your peers. If someone in your field has used the antibody before you ...peace of mind as you make your purchase but also someone to turn to for advice if you need to troubleshoot...