We narrowed to 869 results for: SOM

...myself would take less time than the seemingly burdensome task of explaining the steps to a lab assistant...lab scientist, I worked with a diverse team where some members thrived on independence, while others required...dynamics. I experienced this firsthand when it took me some time to adapt to the communication style of a new...do this while changing industries and positions. Some people discover they truly enjoy management and ...

...month formal program. After the formal program ends, some 50% of these groups continue to meet regularly, ...development plans. However, they find it awkward to ask someone for the time commitment of a formal mentoring relationship...stage, they have a lot to learn from each other. Some have taken my advice and I recently heard from peer...organizations as it brings a diversity of perspective. Sometimes it is helpful to find peers that share a similar...

...itself: Ma et al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic...term ‘editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, ...seems impossible with current tools, thus quieting some fears about the creation of “designer babies.” The... a ‘magic bullet’: its use will always come with some uncertainty. As biologists equipped to understand...

...so-called “small molecule degraders” recruit the proteasome to destroy targeted proteins. We previously developed...polyubiquitination of the target protein and proteasomal degradation. Remarkably, small molecule degraders...dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We are particularly...between the FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon, leading...

...ones to try?  Read on and we will provide you with some advice on the antibody selection process. Antibodies... is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated...used in a publication. Talk with your peers. If someone in your field has used the antibody before you ...peace of mind as you make your purchase but also someone to turn to for advice if you need to troubleshoot...

...as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies...much easier said than done! Each technique, and sometimes each individual assay, will need to be developed...attaching to a single primary antibody, it is sometimes assumed that the direct method is more quantitative...improve direct methods and avoid the indirect ones. Some Western blots, for example, use a primary antibody...

...like light or color. In this post, we’ll go over some of the common conjugates you may encounter and how...conjugates in an earlier post, where you can find some additional tips for working with these tools.          ...chemistry  Whatever the conjugate, the molecules must somehow be tightly linked to your antibody. This chemical...up to 80 per antibody (Mueller, et al., 1988). If some of those lysines are in the antigen binding region...

...panicked silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in...options were to stop talking until I could present something coherent or to quickly string together all the...noteworthy and memorable and awful. So try to keep some perspective. Most people won't notice, or won't ... above to make a strategy that can help. Even something as simple as telling yourself, “I can stop and...

...needs to be empirically determined. In addition, some metal ion substrates, such as those containing Co2...to the metal ions once the protein is folded. Sometimes a tag placed on one end will be blocked during...not disrupted by denaturants (Hochuli, 1988). In some cases, proteins can be bound under denaturing conditions... Introduction to Antibodies.) Unfortunately, for some proteins there just aren’t any good antibodies available...

...are encoded by single stranded RNA (ssRNA), while some exist as dsRNA. Still others use dsDNA to store ...know the sequence of a virus, you could introduce some of its dsRNA intermediates into a healthy individual...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...documented in several autoimmune disorders, including some forms of lupus and rheumatoid arthritis. These ...

... structure? Helping to answer some of these questions, and posing some of their own are the following....   The news is not all bad, however - there are some signs that the tides may be turning. In 2013, the...- this time with the aim of finding solutions to some of the problems that were identified last year.  ...

...learn about the tools available for bacteria and some of the applications for which they’ve been used....Different Cas orthologues and subtypes function somewhat differently and can be more effective targeting...and CRISPRa using RNAP-Omega-dCas9 fusions. Here’s some others. SoxS based CRISPRa In the search for a CRISPR...Cytidine and adenine base editing in Streptomyces Some of the most industrially and clinically relevant...10.1016/j.cell.2013.02.022 Ronda C, Pedersen LE, Sommer MOA, Nielsen AT (2016) CRMAGE: CRISPR Optimized...

...conclusion that genes encode proteins and reside on chromosomes, which are composed of DNA. These findings culminated...have also expanded the genetic code by redirecting some codons to encode for synthetic amino acids. Plasmids...molecules. To isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...information and can replicate independently from chromosomal DNA. Although they can be found in archaea and...horizontal gene transfer called conjugation. Like chromosomal DNA, plasmid DNA is replicated upon cell division...DNA from one context to another, such as from a chromosome to a plasmid or vector DNA backbone. These tools...

... measuring locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress...adult animals, and not targeted to a specific area. Some other technical considerations for stereotaxic injections...via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency can...depression. In upcoming blog posts, we’ll discuss some of these behavioral studies in detail and share ...

...resolution. Stories usually have a theme too, which is something not inherently captured by the STAR method. I ...interview. Each interview is an opportunity to learn something and then apply it to the next interview. Perhaps...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...questions after completing each section of your talk. Some video conferencing software allows participants ...

...Every few months we highlight some of the new plasmids, antibodies, and viral preps in the repository... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA... achieved high editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant... interest, its dimeric nature can interfere with some applications. Several labs have been working to ...

...about? Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication of popularity...section of our plasmid pages is relatively new, so some of our older plasmids may not have an entry in this...Ordering: Check the Price of course! The price of some items vary, for example, plasmid kits are avaliable...store plasmids that need extra stability, such as some of our viral vectors, in NEB Stable cells. Resource...

...right Tn7 end sequences. Donor bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these...and recipient bacteria that lack an integrated chromosomal copy of Mobile-CRISPRi. For firmicute gram positive...tri-parental mating. Donor bacteria contain a chromosomal copy of the RP4 transfer machinery, which mobilizes... 1 transfer in every ~100 to 1,000 colonies, but some bacteria, including E. faecalis and P. mirabilis...

...never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit intimidating...intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools...definitely made my life easier!  In this post, I’ll share some AAV production, purification, and titration tips...to create the iodixanol gradient layers, and has some great pointers that while help you master iodixanol...

...that forms chromosomes. These long range DNA interactions are often detected with chromosome conformation...factors, can increase a gene’s transcription. Sometimes enhancers are located thousands of base pairs ...created for each genomic loci of interest. This is cumbersome and also makes it hard to compare results across...