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We narrowed to 949 results for: ache

Showing: 341 - 360 of 949 results

  1. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...number of papers with your search term published each year, which is a nice way to see growth or decline... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ...the number of papers with your key term published each year is displayed with your search results and can...your feed. Feeds are organized by papers published each day, week, or month. A measure of the number of ...

  2. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...supervisor, or do you have to work with difficult people? Each time I ask this question at my workshops, I get ...trouble making decisions. In my work as a thesis coach for graduate students, I heard about supervisors...work hours. While specific strategies to deal with each of these personality types is beyond the scope of...and if you cannot resolve a problem on your own, reach out for help so you can get the support you need...Find plasmids to Expedite Your Experiments and Achieve Your Research Goals    ...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized strategy uses...CC-BY-NC-ND 4.0 International license. With this approach, the team was able to drive prime editing in both...inherited the genomic edit but not the editing machinery. Together, these advances have the potential to...developed a cost-effective expression and labeling approach using these constructs in HEK 293 cells (Smith...) biosensors (Janicot and Maziarz et al., 2024). Each plasmid expresses all the components of the biosensor...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody candidate. These ...have gotten a lot of press for being a promising approach for treatment or prophylaxis of COVID-19. Researchers...nanobody structures (concave, loop, and convex). Each of these 3 mRNA libraries have a concave, loop, ... for in-vitro translation of the mRNA libraries. Each mRNA molecule in the library doesn’t encode a stop...

  5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols... disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers two...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...wild-type bacteria and the relative frequency of each of the 40 library strains was measured after 6 and...

  6. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...proportionally to the amount of product produced each cycle.  To determine the concentration of the starting...reaction sample into approximately 20,000 droplets. Each droplet contains the material required for PCR amplification... amplification. Following the PCR reactions each droplet is analyzed by a droplet reader, which measures...measures the fluorescence amplitude of each droplet. The fraction of fluorescent PCR-positive droplets is ... primer pairs that align to the target region of each template are used in one reaction. Multiplex PCR...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...common types of degrons and some of the nuances to each one.   SMASh Small molecule-assisted shutoff (SMASh...self-cleave and the protease and degron remain attached to the tagged protein. In this event, the degron... tag, and the endogenous cellular degradation machinery to function. The dTAG is the smallest tag reviewed...functions – there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron...441. doi: 10.1038/s41589-018-0021-8 Holland AJ, Fachinetti D, Han JS, Cleveland DW. Inducible, reversible...

  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...limited in the level of resolution that they can achieve. This means that regardless of how powerful of ..., you can’t resolve two points within ~250 nm of each other. For reference, individual presynaptic terminals...sample (i.e. cultured cells or tissues).  Step 2: attach anchors to your sample that will link biomolecules...evenly in all directions, aka isotropically. But achieving isotropic expansion depends on how well the gel...especially when paired with complementary experimental approaches. Since it arrived on the scene, ExM has helped...

  9. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...-associated genetic dependencies associated with each ancestry group. Since about 90% of the cell lines...their data.     Fig. 1: Genomic fraction for each of five major ancestry groups for 994 of the Cancer...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...  Rachel Leeson...

  10. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...  Figure 1: (A) Components involved in each type of CRISPR-Transposon system. (B) Mechanism ...Recently, the lab introduced a streamlined expression approach (Fig. 2A), involving two-plasmid or single-plasmid... constructs. (B) High integration efficiencies achieved with VchINT, at two different incubation temperatures...insertions into multiple distinct targets could be achieved (Vo et al., 2020). VchINT was also shown to be...Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, Doudna JA (2020...

  11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...with many of the same drawbacks to each reagent type, so this approach is not suitable for all antibodies...and outlined the considerations to be made during each step. Now that you know the basic principles of ...the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two...detergents, such as Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that...

  12. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... to be able to cross the blood-brain barrier to reach the brain. Delivering the CREATE AAV library via...jumble of fluorescent neurons. A simple but flawed approach to multi-color labeling is to inject a mix of ...was derived from figure 3b in Chan et al.   To achieve the full diversity of colors AND sparse labeling...co-injected. The dose of this AAV can be adjusted to achieve sparse levels of labeling, without loss of color...virus, but the desired sparsity of labeling is achieved with lose doses of inducer virus. Additionally...

  13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...spent this remaining time by elaborately pranking each other, dressing in costume, and holding competitions... we are sending hundreds of plasmid requests out each day. As you may imagine, it keeps all of us quite...furthering our mission by hard work, we genuinely enjoy each other’s ideas and company. The Addgene summer picnic...sometimes work is a bummer when you’d rather be at the beach or binging a new Netflix series. The thing that ...you can enjoy the people you spend 40+ hours with each week. In conclusion - get out there any have some...

  14. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...number of days and tracking average transit time for each courier we contracted with so we can always offer...can see our most popular educational resources for each type of resource we offer.  In 2013, we launched...experimental help. We love it when a researcher reaches out to ask if they can help us create or update... We’ve learned that increased accessibility is achieved through interconnected incremental changes. It... Rachel Leeson...

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    ...Tracking cellular history with protein ticker tape Teaching an old antibody new tricks   New compact, efficient...REcipient Cells Through Envelope Design). This modular approach combines an interchangeable set of fusogenic and...side effects. This platform offers a promising approach for experimental therapies and medical applications... into the TurboID enzyme, Lee and Cheah et al. achieved precise control over when and where the enzyme... bioRxiv — doi: 10.1101/2021.10.13.463862.    Teaching an old antibody new tricks By Ashley Waldron While...

  16. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back...non-integrating RNA virus Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming...encoding reprogramming factors behave as separate extrachromosomal elements in the nucleus of the target cell...Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system where each vector contains a viral origin of DNA replication...Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with research interests in ...

  17. The Challenges of Cell Culture

    Type
    Blog Post
    ...This post was contributed by ATCC Outreach Scientist Nick Amiss. Cell culture in the present day Cell... number, cell line, and date, which you complete each time you passage the cells (as well as recording...liquid nitrogen for your Working and Master stocks of each cell line. Once all Master vials have been thawed...the results are reliable. With this two-pronged approach, the many mistakes of the past can be prevented...desired clinical endpoints.   Nick Amiss is an ATCC Outreach Scientist at LGC Group, London, which is the European...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...anneal with each other, while the 5’ flaps containing the original DNA sequence anneal with each other. Excision...identify dozens or hundreds of particles surrounding each of the highly-visible beads on the cryo-EM grid.... samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be damaged at the air-water...plunge freezing represents a convenient, effective approach to protecting your cryo-EM samples from damage...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... will overcome certain obstacles, alternative approaches, potential collaborations, critiques of your ...and all of the caveats and alternatives for an approach, if asked. The key to a chalk talk is to effectively... the knowledge gap in the field? How will your approach resolve this problem? (2-3 sentences) What.... Identify the key points you want to convey for each section or aim. Make sure those get priority. The... fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance runner, gardener...

  20. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...expression, and viability. Gates are the parameters the machine uses to differentiate between variations in those...tumor cells, immune cells, blood cells and more), each coming with a different size and structural complexity...A) A flow plot showing two stains in relation to each other. Using a quadrant gate, I can divide my cell...cell population into DN, DP and SP (for each stain respectively). (B) Instead of a quadrant gate, I am...occur when a cell is pulsed by the laser. Within each type of scattering, you also discovered that there...
Showing: 341 - 360 of 949 results