...functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Rubinson...gene silencing mechanism. As a tool in mammalian cell systems, silencing is achieved through the delivery...expression; Designed for inducing RNAi in a wide range of cell types, tissues and organisms Luk Parijs 11619 pLB...Hacohen N, Hahn WC, Lander ES, Sabatini DM, Root DE. Cell. 2006. 124:1283-1298. PubMed . A versatile tool ...Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H,...

...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ... time and are potentially heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication...DNA. Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian...genomic loci using fluorescent microscopy in living cells. Design your gRNA sequence to direct the dCas9-FP...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...

...e.g., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read our AAV...AAV can transduce both dividing and non-dividing cells with a low immune response and low toxicity. Although... and polycistronic AAV-1 cap gene in Sf9 insect cells Kotin 65214 pSR657 AAV2 Baculovirus-mediated rAAV... and polycistronic AAV-2 cap gene in Sf9 insect cells Kotin 65215 pSR646 AAV6 Baculovirus-mediated rAAV... and polycistronic AAV-6 cap gene in Sf9 insect cells Kotin 65216 pSR660 AAV8 Baculovirus-mediated rAAV... and polycistronic AAV-8 cap gene in Sf9 insect cells Kotin 92307 pAnc80L65AAP Anc80L65AAP AAV packaging...AAV-BI30, a capsid with tropism for CNS endothelial cells. Deverman 184539 rAAV2/cMG AAV-cMG.QRP AAV packaging...

... by ~3,300-fold in Drosophila cells and ~24,000-fold in human HeLa cells as compared to basal expression...origins, Tet systems function well in mammalian cells, and TRE-containing promoters can be used in the...cumate switch system functions well in mammalian cells, and can be used in the repressible manner described...While originally developed for use in mammalian cells, this system has also been implemented in a variety...transgene permits directed expression of that gene in cells that also express GAL4. Usage of this system revolutionized...reversible by the addition of quinic acid to Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also.... Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl ...

...epigenome editing. Cell, 184(9), 2503–2519.e17. doi: https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant...tissue is identifying the cells you are studying. Antibodies can help reveal cellular identities by targeting...particles (eVLPs) offers an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain... will only be transiently present in transduced cells, limiting off-target editing. Cargo size limits ...site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime ... Recombinant antibodies for marking cell types in the nervous system By Ashley Waldron The mammalian nervous... nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who...

... Modeling Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer...Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University...University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute...

...experiment, target cells are treated with the pooled library to create a population of mutant cells that are then...quantities of cells and therefore, may not be suitable for use when the number of cells is limited (e....ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini and Lander 3rd 10 Varies FNLCR CRISPRa Cell Surface...e.g. primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target... target cells. Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you ...organism, and the library is only compatible with cells derived from that organism. All of our current pooled...140219 Knockout Human J. Chen 3rd 10 4,530 Murine Cell Surface Protein Knockout (McspKO) Library 200445...

...genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013 Aug...Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep...CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can ...recombination and fluorescence assisted sorting of cells and nuclei in addition to sgRNA expression. This...

...proteins enable the observation of proteins in living cells and organisms. Both components of the chimera are...However, these may not reflect the situation in cells. Therefore, the localization of critical fusions...currently the standard for assessing oligomerization in cells. In addition to measuring stickiness, several researchers...fluorescent proteins that behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet...protein to the existing pool of protein within a cell and may lead to over-expression artifacts. This ...heterotrimeric G protein Gq activation in living cells." BMC biology 9.1 (2011): 32. PubMed PMID: 21619590...activation of heterotrimeric G-proteins in living cells." Science 291.5512 (2001): 2408-2411. PubMed PMID...

...its oxidized derivatives. Cell 152, 5 P1146-1159. https://doi.org/10.1016/j.cell.2013.02.004.  Xu X, Tao...targeted DNA demethylation. Cell Discov 2, 16009. https://doi.org/10.1038/celldisc.2016.9.   ...enzymatic activity cause demethylation?  Dividing cells depend on DNA methyltransferase 1 (DNMT1) to copy...methylate sites blocked by dCas9. Within a few rounds of cell division, the original methylated strand is diluted...dCas9 expression and pLM-CMV-R-Cre. Selection for cells stably and highly expressing these constructs can...can be achieved by fluorescence-activated cell sorting, and stable gRNA expression can be achieved using...TETs compete with DNMT3 activity in pluripotent cells at thousands of methylated somatic enhancers. Nat...

...different types of human cells, including T cells and hematopoietic stem and progenitor cells. Now with the DAP...to enable multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads... over 50% averaging editing efficiency in human cells. MBE with DAP array also exhibited reduced Cas9-... editing, and nuclease editing, in virtually any cell type or organisms of interest with only minimal ...

... used HEK293T cells in our first study, but we're planning to soon expand to other cell types, including...26 of the 29 SARS-CoV-2 viral proteins in human cells and used these viral “bait” proteins to fish for...human proteins that the virus interacts with in cells. Because viruses rely on the help of human proteins...monkeywrench into the virus’ hijacking of human cells. Even better, more than half the drugs hitting these...they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven by a fairly...

...expression targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches...approaches excel at discrete cell or tissue-subregion targeting down to the limits of light delivery. ...-wide targeting, as the ligand can reach target cells throughout the body. Controlling Stimulation Some... and chemogenetic tools need to be delivered to cells, commonly through viral delivery by AAV. You can...Further restricted to illuminated region Impacts all cells expressing receptors Invasiveness Mice require...

...potential to form aggregates and may be toxic to the cell. Here comes the SunTag How does SunTag fix these...enables single molecule tracking  within living cells without affecting protein function, thereby creating...attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, they found...SunTag constructs would avoid negative effects on cell physiology. Having seen that mitochondrial tracker...amplification in gene expression and fluorescence imaging.” Cell 159(3) (2014):635-46. PubMed PMID: 25307933. PubMed...time to get enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of...creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum et al. observed some...

...shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological research...turnover rate. In many cases, however, a GFP-tagged cell line or organism has already been made, and it would...made by accident. After exciting GFP-labeled plant cells at 405 nm instead of 488 nm, Sattarzadeh et al. ...spontaneous conversion did not occur. Tobacco suspension cell culture with cytosol-targeted GFP. White circles...photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other FPs... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that...

...already available today to cure sickle cell anemia in stem cells but this can’t be used in people until... audience discussed how J&J is already modifying cell and animal genomes to test hypotheses of disease...and more about the how to use genome edited stem cells to treat disease. As for me, I continue to be amazed... much we have yet to learn. As Dr. Mello said, “Cells have been in the information age for billions of...

...of fluorescent proteins and their use for imaging cell biology, Michael Davidson’s lab at Florida State...blue, and orange spectra. A comprehensive list of cell markers all tagged with mEmerald. Plasmids to monitor...From F-Actin to mitochondria, from the nucleus to cell membrane, this collection has fluorescently tagged... where your protein of interest localizes in the cell, use these markers as your guide. As Michael Davidson...Fluorescent proteins are] a whole new way of seeing cell biology, seeing what interacts with what, what affects...of many proteins known to localize to specific cellular structures.The Davidson lab even included some...everywhere to get a clearer picture of that intracellular world, even as the Davidson lab prepares to ...

...Collection Allen Institute for Cell Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection...Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase CUREs ...

...suitable for virus purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly..., iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient media...

...samples may not have been processed correctly in the cell (e.g., failure to cleave the signal peptide) and...Scatter chart. Change the data range and select the cells where the x-axis is represented. Add a linear trendline...