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TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each gene page...plasmids for the gene. For components with multiple isoforms or subunits, individual links to each gene page...
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CRISPR Plasmids - Mammalian Expression
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
Fluorescent Protein Guide: FRET
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...experiments to measure protein interactions or conformation changes, as well as FRET standards. Plasmid... intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the donor and... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...Cell Junctions Cell junctions are responsible for forming connections and adhesions between animal cells....added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post... EB-1B requires that you provide two out of six forms of documentary evidence: Evidence of receipt of... of this post are intended to convey general information only and not to provide legal advice or opinions...particular circumstance or fact situation. The information presented may not reflect the most current legal...No action should be taken in reliance on the information contained in this post and we disclaim all liability...intentions directing federal agencies to propose reforms to the H-1B visa system. Large firms and firms ...to most postdocs. Commercial successes in the performing arts- not relevant at all. Please do not include...you are a classically trained ballerina who has performed at Lincoln Center. Your case should only focus... -
CRISPR Plasmids - Plants
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...fill out our form to let us know what you would like to see available in viral prep format. Please note...plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers. All buffers...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the...Please click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970...(CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When using the AAV2(Y444F) serotype... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... including (1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting module with the crRNA... of bacteria including in pooled, genome-wide, formats. Interested in knowing more? Check out our Mobile... WA (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression... -
TALEN Plasmids and Kits
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
Control AAV Preps
TypeCollection...fill out our form to let us know what you would like to see available in viral prep format. Please note... -
Lentivirus Plasmids
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...transgene or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer... -
Tetracycline Inducible Expression
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert...transgene or shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB...expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional selection... -
CRISPR Plasmids - Tagging
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...described below. Read our CRISPR 101 post for more information about HDR/repair template design. How to use ... -
15 Hot Plasmids from 2017
TypeBlog Post...long fluorescence lifetime, and a stable monomeric form as determined by the organized smooth endoplasmic... 9 amino acid N-terminal variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that ...Polycistronic cassettes that contained the KLF4L isoform had more robust initiation of reprogramming (evaluated...All three mScarlet variants have shown great performance in cellular functional imaging and in protein... These Lmo proteins were identified using a bioinformatics approach examining genomes possessing CRISPR... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...Protein phosphorylation is one of the most abundant forms of posttranslational modifications in cells and ... is anticipated to be useful for laboratories performing phosphoproteomic experiments in need of reference... -
Plan Your Experiment
TypeCollection...protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex, which is delivered to cells... be used to examine off-target effects. More information on each of these techniques can be found in our... -
Optogenetics AAV Preps
TypeCollection...fill out our form to let us know what you would like to see available in viral prep format. Please note...Plasmids and Optogenetics Guide page for more information about this field. Use the table and filters below... -
Retrograde AAV viral preps
TypeCollection...fill out our form to let us know what you would like to see available in viral prep format. Please note... vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control... -
Caltech Systemic Capsids
TypeCollection...fill out our form to let us know what you would like to see available in viral prep format. Please note...Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please refer...window) ) to cross the blood brain barrier. For information on whether the PHP.eB serotype will work in your...pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in future publications... -
CRISPR Guide
TypeCollection...ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions...effector, composed of several separate Cas enzymes that form a complex called Cascade (see Cas3 for more details...with Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation...dCas9 Nuclease dead Cas9, an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble...Cas9 undergoes a second conformational change upon target binding. In this formation, its nuclease domains...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into an active,...