Skip to main content

We narrowed to 523 results for: genome

Showing: 341 - 360 of 523 results
  1. Zinc Finger Consortium Reagents

    Type
    Collection
    ... these reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish...expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents...
  2. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...purity testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged...
  3. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...dCas9-FokI to dimerize at a given region of the genome. Like nickase technology, this technique reduces...
  4. Retrovirus Plasmids

    Type
    Collection
    ...of retrovirus and contain a viral genome encoded on RNA. The genome includes a reverse transcriptase and... and an integrase to insert the viral genome into a host cell. Scientists can take advantage of this to...
  5. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...which can become costly and impractical for whole-genome (and often, whole-plasmid) sequencing.  Prior to...well as the ability to sequence an entire viral genome in one read. We also use Nanopore sequencing to...
  6. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...alone, and this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now...22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial plasmids: classification and identification...
  7. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...  IPTG-inducible expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ:...expresses T7 RNA polymerase (RNAP) from the bacterial genome under control of the lac repressor, which is inducible...
  8. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. Ahmed A. Gomaa, et...
  9. TALEN Plasmids and Kits

    Type
    Collection
    ... Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering... Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like...target DNA sequences in living cells (TALE-mediated Genome Visualization [TGV]). pTALYM3 and pTALYM4 contain...this page are designed to target and edit the epigenome of putative enhancers in mammalian cells. These...target locus ( Mendenhall et al., 2013 ). This epigenome editing was also shown to alter regulation of ...
  10. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... plasmids for CRISPR-Cas and Cascade for large genomic deletions....into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions generated by Cas9...
  11. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...multiple components) to target locations in the genome defined by a guide RNA. The transposase can then...insertions. CASTs are most often used in bacteria genome editing, though some mammalian systems have been...
  12. TALEN Engineering

    Type
    Collection
    ... Genome Engineering TALEN Kits TALengineering Reagents ...TALengineering website. Users may also wish to join the Genome Engineering newsgroup. An upgraded version of the...REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome editing...
  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...flanking regions comprised of upstream and downstream genomic sequences (Figure 2B).   Figure 2: A comparison... boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. When it...
  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (...Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org Checkout...
Showing: 341 - 360 of 523 results