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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 535 results for: genome

Showing: 341 - 360 of 535 results

  1. Synthetic Biology - Cloning and Genomic Tools

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    ...Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing...plasmids related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems...for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools Synthetic...Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly Standards... systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by the...

  2. CRISPR Guide

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    ...that defines the genomic target to be modified. You can therefore change the genomic target of the Cas...of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-...not permanently modify the genomic DNA. dCas9 has also been used for genome-wide screens to activate or...visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify Genomic Regions ...RNAs), and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic regions using...

  3. CRISPR Plasmids for Genomic Visualization

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    ...programmable for different genomic loci, capable of detecting multiple genomic loci, and compatible with...non-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific genomic locus... visualization of genomic loci. CRISPR... CRISPR Visualize CRISPR Plasmids: Visualize Genomic Loci Browse CRISPR...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...with fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over...sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple...

  6. Antibodies 101: Epitope Tags

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    Blog Post
    ... the tag, and are introduced via knock-in at a genomic locus or more commonly via cloning of recombinant...

  10. CRISPR Plasmids - Purify Genomic Loci

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    ...CRISPR Purify CRISPR: Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick... Posts Identifying molecules associated with a genomic region of interest in vivo is essential to understanding...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the...catalytically inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be...epitope tag. Compared to conventional methods for genomic purification, CRISPR-based purification methods... identification of molecules associated with a genomic region of interest in vivo. For CRISPR-based purification...tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in ...

  11. CRISPR References and Information

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    ...off-targets, target's genomic location, and genome annotation. Available genomes are human (hg19 & hg38...design tool for genome editing, as well as gene regulation (repression and activation). Genome support for...-use computational pipeline for the analysis of genome editing experiments. It enables rapid and intuitive... a new window) . MAGeCK Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can...selected sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology...prediction. Developed by the Hyongbum Kim Lab . GenET (Genome Editing Toolkit) (Link opens in a new window) A...well as predict off-targets, for CRISPR in 180+ genomes. Developed by the Jean-Paul Concordet Lab . For...

  12. CRISPR Pooled gRNA Libraries

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    ...123,411 Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406 Human improved genome-wide library...Mouse genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide ...Pooled Libraries Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...more complex than using CRISPR to modify a single genomic locus. Before ordering, please review the steps... - A given CRISPR library may target the entire genome or a specific class of genes (subpools). Species...within a given CRISPR library are unique to the genome of particular organism, and the library is only...Herpes Virus (KSHV) Cow ( B. taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear ...

  13. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ...are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs are used to knockdown expression... We will need information on how to verify any genomic alterations unique to your strain. Please contact...

  14. Zhang Lab CRISPR Page

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    ...are below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to ...vectors for genome editing in the brain Using Cas9 mice Platt et al. demonstrated in vivo genome editing ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al ., Science...libraries (both human and mouse) for performing genome-wide knockout screens. The libraries are available...Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab has both single- and dual-vector...top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of...

  15. Plan Your Experiment

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    ...sequence and your genomic target may reduce the gRNA activity. Be sure to sequence the genomic region you intend...system that enables researchers to manipulate the genome with relative ease. Over the past decade, researchers...Homology Directed Repair (HDR) Generate specific genomic edits, like point mutations or small tag insertions...gene expression without permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional...gene expression without permanently modifying the genome sequence dCas9 fusion with transcriptional activator... chosen Cas enzyme at the right spot within the genome relies on proper gRNA design. While there are some...sequence with no homologous sites elsewhere in the genome. In reality, a given gRNA target sequence will ...

  18. Plasmids 101: Inducible Promoters

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    Blog Post
    ...developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila...
Showing: 341 - 360 of 535 results