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We narrowed to 539 results for: genome

Showing: 341 - 360 of 539 results

  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... the tag, and are introduced via knock-in at a genomic locus or more commonly via cloning of recombinant...

  7. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ...systematically perturbs the genome to assess the impact of genomic variation on genome function and phenotype...Consortium investigators to examine how genomic variation affects genome function to influence phenotypes. ...new window) aims to understand how genomic variation affects genome function, which in turn impacts phenotype...and map how genome function shapes phenotype, and how these processes are affected by genomic variation....will produce a catalog of the impact of genomic variants on genome function and phenotypes. Plasmids The...Depositor Collections IGVF Consortium Impact of Genomic Variation on Function (IGVF) Consortium Collection...window) (Link opens in a new window) The Impact of Genomic Variation on Function (IGVF) Consortium (Link opens...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs are used to knockdown expression... We will need information on how to verify any genomic alterations unique to your strain. Please contact...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...that cleaves the genomic DNA. Type II CRISPR was the first system harnessed for genome engineering, with...anti-CRISPRs as off-switches for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek...valuable genome engineering tool. CRISPR... CRISPR CRISPR History CRISPR History and Development for Genome Engineering...prokaryotic immune system is especially amenable to genome engineering, offering flexibility and easy multiplexing...more so) for research applications, eclipsing past genome engineering technologies like zinc finger nucleases...sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard against...

  10. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing...plasmids related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems...for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools Synthetic...Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly Standards... systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by the...

  11. CRISPR Guide

    Type
    Collection
    ...that defines the genomic target to be modified. You can therefore change the genomic target of the Cas...of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-...not permanently modify the genomic DNA. dCas9 has also been used for genome-wide screens to activate or...visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify Genomic Regions ...RNAs), and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic regions using...

  12. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...programmable for different genomic loci, capable of detecting multiple genomic loci, and compatible with...non-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific genomic locus... visualization of genomic loci. CRISPR... CRISPR Visualize CRISPR Plasmids: Visualize Genomic Loci Browse CRISPR...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...with fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over...sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple...

  13. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Vector Type Expression Genome Packaging Capacity Virus Size Cells Infected Host Genome Integration Immune ...expression and are permanently integrated into the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated...expression, but generally don't integrate into the host genome. AAVs are limited by their small packaging capacity...cells. They integrate permanently into the host genome, enabling long-term transgene expression. Retroviral...vectors also vary in the composition of their viral genome, expression of the transgene, packaging capacity...Recombinant AAV has a low frequency of target cell genome integration. Introduction to Viral Vectors Figure... goal, which is to make more viruses. The viral genome contains only the necessary genes to achieve this...

  16. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila...
Showing: 341 - 360 of 539 results