Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 949 results for: org

Showing: 341 - 360 of 949 results

  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...process by which cytoplasmic material, including organelles, are targeted to lysosomes for degradation. Autophagy...provides limited information about what materials or organelles are being targeted for autophagy. The development...for reporting vacuolar turnover of cytosol and organelles in yeast." Autophagy 4.2 (2008): 205-213. PubMed... sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent...

  2. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working with viruses...please email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated... we distribute, please send an email to help@addgene.org. Your questions help us expand and improve our...Expression in Mammals Additional Resources on Addgene.org Overview of Viral Production at Addgene Find...

  3. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...Staining. J Histochem Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (...Sections. J Histochem Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813  Tuson JR, Pascoe EW...Cytochem : Off J Histochem Soc 38:923–926. https://doi.org/10.1177/38.7.2355173  Additional resources on the...Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene   ...

  4. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran WF, Lam...severity and survival. Cell 184:476-488.e11. https://doi.org/10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam...humoral immunity. Cell 184:2372-2383.e9. https://doi.org/10.1016/j.cell.2021.03.013 Additional resources... Learn more about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection...

  5. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun A (2015)...recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein ND, Thie...functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Additional resources...recombinant monoclonal antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene...

  6. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict...Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L,...Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276 Additional resources on the...cell-type expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV...

  7. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard LH, Smith O, Tilmans...Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke...Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional resources...viral vectors protocols and tips Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's...

  8. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science...Archives will help both you and your lab stay organized allowing you to upload anything from protocols...came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics...Develops Lab Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...Please send any questions or comments to help@addgene.org. Finally we’d like to thank all those who are...Viral Transductions Additional Resources on Addgene.org Visit the Viral Service Page Check Out Our CRISPR...

  10. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...manuscripts: literature searching, reading, and organizing Tips for writing a scientific review article   ...

  11. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...

  12. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare publications...publications or your dissertation Organize data that you already collected into figures for papers or...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...

  13. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization and expression of genetic determinants for synthesis...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

  14. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... be found on its website (biobuilder.org) or by emailing info@biobuilder.org. Of particular interest this...found through experiential learning. “Students may forget the technical details they hear in their classes...All Synthetic Biology Blog Posts Resources on Addgene.org Guide to SynBio Assembly Standards Find Plasmids...

  15. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ... work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ... survival. For those interested in development, organs of interest, such as the intestines, can be dissected...have!) (4). The power of Drosophila as a model organism is in its amenability to genetic manipulation....for Site-Specific Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...detract from the process being depicted, we can’t forget that those other components are still there in ... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...In-exhaustive Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A ...

  17. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...Science, 343(6166): 80-84 (2014). doi: https://doi.org/10.1126/science.1246981. Leemhuis, H., Kelly, R.M.... IUBMB Life, 61: 222-228 (2009). doi: https://doi.org/10.1002/iub.165.  Additional resources on the Addgene...

  18. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...18(2), 67–83. https://doi.org/10.1038/s41579-019-0299-x Resources on Addgene.org CRISPR software and...information on Class 2 Cas enzymes. The information is organized in wiki format and includes biochemical properties...Biochemistry, Biophysics, and Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I...

  19. Hot Plasmids - February 2022

    Type
    Blog Post
    .... 2021. https://doi.org/10.1111/gtc.12893, Zhao et al. Nat Comm. 2019. https://doi.org/10.1038/s41467-...Santilli, R.T., et al. Sci Rep (2021). https://doi.org/10.1038/s41598-021-96051-x Engineered miniature...Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org/10.1016/j.molcel.2021.08.008. ...

  20. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...your current organization but also important to build relationships outside your organization. The mentor...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...
Showing: 341 - 360 of 949 results